KnE Life Sciences
ISSN: 2413-0877
The latest conference proceedings on life sciences, medicine and pharmacology.
Characteristics of Mayonnaise with Soybean Oil Substitution and Various Types of Egg Yolks
Published date: Aug 12 2025
Journal Title: KnE Life Sciences
Issue title: The 6th International Conference on Technology, Education, and Social Science (6th ICTESS): Science and Technology
Pages: 278 - 292
Authors:
Abstract:
Mayonnaise is a semi-solid oil-in-water (o/w) emulsion product with a high oil content, in which egg yolks play a key role as natural emulsifiers. This study aimed to determine the chemical, organoleptic, and droplet size properties of mayonnaise produced using various combinations of palm oil and soybean oil, along with different types of egg yolks, to develop a high-protein mayonnaise preferred by consumers. A completely randomised design (CRD) was used, with two factors: 1. the ratio of soybean oil and palm oil (40:60, 50:50, and 60:40), and 2. The type of egg yolk (laying hen, backyard chicken, and duck). The best result was found to be the 40:60 palm oil to soybean oil ratio combined with backyard chicken egg yolk, resulting in mayonnaise with 66.07% fat, 12.70% moisture, 4.75% protein, 2.39 dPaS viscosity, 99.80% emulsion stability, pH of 4.31, and an overall preference score of 3.73. Also, formulations using laying hen and backyard chicken egg yolks produced smaller, more tightly packed fat globules, enhancing emulsion stability. Backyard chicken egg yolk notably improved protein content and emulsion quality in mayonnaise.
Keywords: mayonnaise, palm oil, soybean oil, egg yolks
References:
[1] Evanuarini H, Nurliyani I, Hastuti P. Characteristic of Low Fat Mayonnaise Containing Porang Flour as Stabilizer. Pak J Nutr. 2015;14(7):39.
[2] Rusalim MM. Tamrin, Gusnawaty. Analisis Sifat Fisik Mayonnaise Berbahan Dasar Putih Telur dan Kuning Telur dengan Penambahan Berbagai Jenis Minyak Nabati. J Sains Dan Teknol Pangan. 2017;2:507–58.
[3] Kantekin-Erdogan MN, Ketenoglu O, Tekin A. Effect of monoglyceride content on emulsion stability and rheology of mayonnaise. J Food Sci Technol. 2019 Jan;56(1):443–50.
[4] Cambero MI, Cabeza MC, Ordó nez JA, de la Hoz L. Effect of E-beam treatment on the safety and shelf life of mayonnaise potato salad. Foodborne Pathog Dis. 2011 Feb;8(2):221–9.
[5] Menchetti L, Taticchi A, Esposto S, Servili M, Ranucci D, Branciari R, et al. The influence of phenolic extract from olive vegetation water and storage temperature on the survival of Salmonella Enteritidis inoculated on mayonnaise. Lebensm Wiss Technol. 2020;129:109648.
[6] Winarno FG. Pangan Gizi, Teknologi dan Konsumen. Jakarta: Gramedia Pustaka Utama; 1993.
[7] Shen Y, Chang C, Shi M, Su Y, Gu L, Li J, et al. Interactions between lecithin and yolk granule and their influence on the emulsifying properties. Food Hydrocoll. 2020;101:105510.
[8] Fromm D, Gammon SU. Specific gravity and volume of the hen’s egg yolk as influenced by albumen pH and storage age of the egg. Poult Sci. 1968 Jul;47(4):1191– 6.
[9] Dixit A, Sharma T. Soybean constituents and their functional benefits. Res Signpost. 2011;661(2):367–83.
[10] Ivano L. Sredanovic. Fatty acid composition of various soybean products. Food Feed Res. 2010;2:65–70.
[11] Marliyati SA, Harianti R, Studi Kesehatan Masyarakat P, Tinggi Ilmu Kesehatan Al Insyirah Pekanbaru S. Karakteristik Fisikokimia Dan Fungsional Minyak Sawit Merah. JGMI J Indones Community Nutr. 2021;10:2021.
[12] Setiawan AB, Rachmawan O. Denny Suryanto Sutardjo. Pengaruh penggunaan berbagai jenis kuning telur terhadap kestabilan emulsi, viskositas dan pH mayonnaise. Student-e J. 2015;4:1–7.
[13] Amertaningtyas J. Sifat Fisiko Kimia Mayonnaise dengan berbagai Tingkat konsentrasi minyak nabati dan kuning telur ayam buras. J Ilm Ilmu Pendidik. 2011;21(1):1–6.
[14] Huang L, Wang T, Han Z, Meng Y, Lu X. T. Wang Z, Han YM, X. Lu. Effect Of Egg Yolk Freezing On Properties Of Mayonnaise. Food Hydrocoll. 2016;56:311–7.
[15] Usman NA, Wulandari E, Suradi K. Pengaruh Jenis Minyak Nabati Terhadap Sifat Fisik dan Akspetabilitas Mayonnaise. J Ilmu Ternak. 2016;15:22–7.
[16] Yenrina R. Metode Analisis Bahan Pangan Dan Komponen Bioaktif. 2015.
[17] AOAC. Association of Official Analytical Chemists. 18th ed. Washington (DC): Association of Official Analytical Chemists; 2005.
[18] Fransisca Y, Santoso B, Sarungallo ZL. Pengaruh jenis pengemulsi terhadap karakteristik fisik, total karotenoid dan sifat organoleptik mayones dari minyak buah merah (Pandanus conoideus Lamk.) degumming. Agrointek J Teknol Ind Pertan. 2023;17(3):706–15.
[19] Ernisti W, Riyadi S, Jaya FM. Karakteristik biskuit (crackers) yang difortifikasi dengan konsentrasi penambahan tepung ikan patin siam (Pangasius hypophthalmus) berbeda. J Ilmu-Ilmu Perikan Dan Budid Perair. 2018;13(2):88–100.
[20] Ketaren. Pengantar Teknologi Minyak dan Lemak Pangan. Jakarta: (UI-Press (ed.)).; 2016.
[21] Wiyani L, Aladin A, Sabara Z, Mustafiah M, Rahmawati R. Rahmawati. Pengaruh waktu dan kecapatan homogenisasi terhadap emulsi virgin coconut oil-sari jeruk dengan emulsifier gum arab. J Chem Process Eng. 2020;5(2):2655–967.
[22] Nurfiqih D, Hakim L, Muhammad. Pengaruh suhu, persentase air dan lama penyimpanan terhadap persentase kenaikan asam lemak bebas (ALB) pada crude palm oil (CPO). J Teknol Kim Unimal 2021;10:01–14.
[23] Gaonkar, Koka KC, B C. Emulsifying Functionality of Enzyme Modified Milk Proteins in O/W and Mayonnaise-Like Emulsions. Africans J Od Food Sci. 2010;4(1):16.
[24] Hutapea CA, Rusmarilin M. Nurminah. Pengaruh perbandingan zat penstabil dan konsentrasi kuning telur terhadap mutu reduced fat mayonnaise. J Rekayasa Pangan Dan Pertan. 2016;4(3).
[25] Rahmawati D. Jenis asam lemak minyak nabati memengaruhi karakteristik sensori mayonnaise. Bogor: Skripsi. Fakultas Teknologi Pangan. Institut Pertanian Bogor; 2016.
[26] Zhong G, Meng F, Li YC, Liu DY, Guo XQ, Zheng L. Structure and rheological charaterization of konjac glucomannan octenyl succinate (KGOS). Food Hydrocoll. 2018;77:386–96.
[27] Siregar RF, Hintono A, Mulyani S. Perubahan Sifat Fungsional Telur Ayam Ras Pasca Pasteurisasi. Anim Agric J 2012;Vol. 1 No.
[28] Soekarto. Teknologi penanganan dan pengolahan telur. Bandung: Alfabeta; 2013.
[29] Winarno FG. Kimia Pangan dan Gizi. Jakarta: Gramedia Pustaka Utama; 1997.
[30] Evanuarini H, Nurliyani I, Hastuti P. Kestabilan Emulsi dan Karakteristik Sensoris Low Fat Mayonnaise dengan Menggunakan Kefir sebagai Emulsifier Replacer. J Ilmu Dan Teknol Hasil Ternak 2016;Vol 11:Hal 53-59.
[31] Biradar SV, Dhumal RS, Paradkar RA. Rheological investigation of self-emulsification process: effect of co-surfactant. J Pharm Pharm Sci A Publ Can Soc Pharm Sci Soc Can. Sci Pharm. 2009;12(2):164–74.