Published date: Dec 20 2024

Journal Title: West Kazakhstan Medical Journal

Issue title: West Kazakhstan Medical Journal: Volume 66 Issue 4

Pages: 365 - 372

DOI: 10.18502/wkmj.v66i4.17768

Nadiar M. MussinDepartment of General Surgery, West Kazakhstan Marat Ospanov Medical University, Aktobe

Akmaral BaspakovaDepartment of Epidemiology, West Kazakhstan Marat Ospanov Medical University, Aktobe

Kulyash R. ZhilisbayevaDepartment of Languages, West Kazakhstan Marat Ospanov Medical University, Aktobe

Seyyed Mojtaba MousaviDepartment of Chemical Engineering, National Taiwan University of Science and Technology, Taipei City

Reza ShiraziDepartment of Anatomy, School of Biomedical Sciences, Medicine & Health, UNSW Sydney, Sydney

Aigerim A. UmbetovaDepartment for Scientific Work, West Kazakhstan Marat Ospanov Medical University, Aktobe

Amin Tamadonamintamaddon@yahoo.comDepartment of Natural Sciences, West Kazakhstan Marat Ospanov Medical University, Aktobe

Exosomes are nano-sized extracellular vesicles that play essential roles in intercellular communication, carrying biomolecules such as proteins, lipids, and RNAs that can influence physiological and pathological processes. The isolation of pure exosomes is critical for both basic research and clinical applications, including diagnostics and therapeutics. Traditional exosome isolation techniques, such as ultracentrifugation, lack specificity and may yield impure samples, making the need for advanced isolation techniques evident. Ligand-based exosome affinity purification (LEAP) column chromatography has emerged as a novel method that utilizes specific ligands targeting exosome surface markers, providing a highly specific, gentle, and scalable approach to exosome isolation. This mini review explores LEAP chromatography’s mechanism, benefits, and potential for clinical applications, emphasizing its g.rowing importance in exosome-based diagnostics and therapies.

Keywords: exomes, affinity chromatography, biomarkers, drug delivery, systems regenerative medicine

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