Published date: Sep 06 2017

Journal Title: Sudan Journal of Medical Sciences

Issue title: Sudan JMS: Volume 12 (2017), Issue No. 3

Pages: 163-173

DOI: 10.18502/sjms.v12i3.931

Salfo SawadogoSaw_salfo@yahoo.frPietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina Faso

Birama Diarradiarra.birama679@gmail.comPietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina Faso

Cyrille BIsseyecbisseye@gmail.comUniversity of Science and Technique of MasukuDepartment of Biology, Assistant Professor

Tegwindé Rebeca Compaorerebecca23fr@yahoo.frPietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina Faso

Florencia W. Djigmaflodjig@yahoo.frPietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina Faso

Djénéba Ouermioddianemed@gmail.comPietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina Faso

Aboubakar S. Ouattaraouattabs@univ-ouaga.bfCRSBAN, University of Ouaga I Pr Joseph KI-ZERBO, 03 BP 7131 Ouagadougou 03, Burkina Faso

Jacques Simporejacques.simpore@yahoo.frPietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina Faso

ABSTRACT:

Background: Bacteriological diagnosis of Campylobacter spp, Salmonella spp and Shigella spp could be necessary in the case of infectious gastroenteritis syndrome.

The objective of this study was to diagnose concomitantly the three enteropathogenic bacteria by multiplex Real-Time PCR in stool culture samples in Ouagadougou (Burkina Faso).

Materials and Methods: The study was conducted from February 5th to March 9^th, 2013. Two hundred stool samples were received during the study period. The bacteria were identified by bacterial culture following by multiplex Real-Time PCR.

Results: Shigella spp and Campylobacter spp were sought by culture in all 200 samples. Enteropathogenic E. coli was sought only in 37 samples from all children under 2 years old. The bacterial culture was positive in 12 stool samples. Shigella spp and Salmonella spp. were isolated respectively in 5 (2.5%) and 3 samples (1.5%). Enteropathogenic E. coli was isolated in 10.8% (4/37) of the samples tested.

The multiplex real-time PCR identified bacteria in 20 patients, including 17 cases of Shigella spp., 1 case of Salmonella spp. and 2 cases of Campylobacter spp.

Conclusions: This study has highlighted the low frequency of 3 sought bacterial genera in stool samples. It has also demonstrated a significant difference between the culture and the multiplex Real-Time PCR method in the diagnosis of Shigella.

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