Article

Published date: Sep 30 2019

Journal Title:

Issue title: Research in Molecular Medicine (RMM): Volume 7, Issue No. 1

DOI: 10.18502/rmm.v7i1.5255

Ahmad AmouzeshiDepartment of Cardiovascular Surgery, Surgery and Trauma Research Group, Birjand University of Medical Sciences, Birjand, Iran

Seyedeh Zahra MoossaviMedical School, Shiraz University of Medical Sciences, Shiraz, Iran

Seyed Yoosef Javad MoosaviStudent Research Committee, Birjand University of Medical Sciences, Birjand, Iran

Mahmoud ZardastCellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran

Mohammad MalekanehCellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran

Sajad EsmailiStudent Research Committee, Birjand University of Medical Sciences, Birjand, Iran

Abdolghader TanehStudent Research Committee, Birjand University of Medical Sciences, Birjand, Iran

Nasim LotfiCellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran

Maryam MoossaviStudent Research Committee, Birjand University of Medical Sciences, Birjand, Ira

Reyhane Hoshyarreyhaneh.houshyar@gmail.comCellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran; Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA

Background: Some plant extracts can prevent kidney stone formation in a dosedependent manner. In our study, we aimed to investigate the protective role of Cannabis sativa aqueous extraction on calcium oxalate formation in ethylene glycolinduced rats. Materials and Methods: To evaluate anti-urolithiasis, variations of the main risk factors (citrate, phosphorous, calcium, and Ph) have been evaluated in 24-hour urine samples of rats one day before the end of the experiment. Rats were divided into 4 groups: group 1 was administered regular drinking water; group 2 was administered 1% ethylene glycol in drinking water, group 3 was administered 100 mg/kg of Cannabis sativa extract for oral administration by gavage along with drinking water containing 1% ethylene glycol; and group 4 was administered 200 mg/kg of Cannabis sativa extract by gavage along with drinking water containing 1% ethylene glycol. Finally, histopathological slides from the kidney were also evaluated. Results: Results showed that 100 mg/kg and 200 mg/kg doses of Cannabis sativa extract significantly reduced the mentioned risk factors in comparison with ethylene glycol-treated rats (p<0.05). Conclusion: Histopathological slides showed reduced calcifications with extract treatment at 200 mg/kg of Cannabis sativa. Thus, this antioxidant herb can prevent kidney stone formation. These findings pave the way for new therapy.