Article

Published date: Sep 30 2019

Journal Title:

Issue title: Research in Molecular Medicine (RMM): Volume 7, Issue No. 1

DOI: 10.18502/rmm.v7i1.5254

Mansour MoghimiM_salehi@med.mui.ac.irAssociate Professor of Clinical and Surgical pathology, Department of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Hossein Sadeghi TaftiDepartment of Medical Laboratory Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Faezeh NamaziMedical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran

Mansoor SalehiMedical Genetics Research Center of Genome, Mohtasham St., Isfahan, Iran

Background: Gliomas are a common type of the primary brain tumors and account formore than 40% of all central nervous system (CNS) tumors. Glioblastoma (GBM) remainsone of the most fatal human malignancies as it is highly angiogenic. Foretinib is anoral multikinase inhibitor that has been shown to exhibit antitumor activity in previousclinical studies. AURKA and AURKB have been shown to be overexpressed in variouscancers.The purpose of this study was to investigate the effect of foretinib on the expressionof AURKA and AURKB in the T98 cell line.Materials and Methods: In this study, the T98 cell line was selected as an experimentalmodel of glioblastoma. The cultured cells were exposed to different concentrationsof foretinib (5 μM, 10 μM, 20 μM, and 0 μM as control). Following that, we examinedthe changes in the expression of AURKA and AURKB under the influence of foretinibcompared to control using quantitative real-time polymerase chain reaction (qRT-PCR).Results: The expression of AURKA and AURKB were found to be significantly reducedin the foretinib treated group compared to the control. The results demonstrated thatincreasing the concentration of foretinib led to reduction in the expression of both thegenes.Conclusion: These findings indicate that the foretinib can decrease the mRNA levelsof AURKA and AURKB. Thus, we suggest that foretinib may be an effective drug forGBM treatment and can be considered for future studies.