Published date: Oct 02 2024

Journal Title: KnE Social Sciences

Issue title: 4th International Conference in Social Science (4th ICONISS): Urban Innovation

Pages: 181–190

DOI: 10.18502/kss.v9i26.17083

Tri Budiyantitrib006@brin.go.idResearch Centre for Horticulture, National Research and Innovation Agency (BRIN), Bogor

Riska ‎ Research Centre for Horticulture, National Research and Innovation Agency (BRIN), Bogor

Rinda KiranaResearch Centre for Horticulture, National Research and Innovation Agency (BRIN), Bogor

NL. Putu IndriyaniResearch Centre for Horticulture, National Research and Innovation Agency (BRIN), Bogor

E. Darma HusadaResearch Centre for Applied Microbiology, National Research and Innovation Agency (BRIN), Bogor

Sri WahyuniResearch Center for Plants Conservation, Botanic Gardens and Forestry, National Research and Innovation Agency (BRIN), Bogor

Irfan SuliansyahDepartment of Agronomy, Faculty of Agriculture, Universitas Andalas, Padang

Dini HervaniDepartment of Agronomy, Faculty of Agriculture, Universitas Andalas, Padang

Capsicum annum L. is the most widely cultivated species due to its economic importance and health benefits. West Sumatra is one of the largest chili pepper producers in Indonesia, so it has a lot of local chili genetic resources. There is inadequate information on the genetic diversity of local chilies in West Sumatra. The purpose of this research was to analyze the genetic diversity of 23 genotypes of chili pepper from West Sumatra amplified with 10 markers of ISSR. The material used was leaves from 23 genotypes of West Sumatra’s local chili which are collections of the Agriculture Faculty, Andalas University, Padang, West Sumatra. The results showed that ISSR markers (ISSR primer 12, ISSR UBC, and ISSR 3M) had produced 84 polymorphic DNA bands with a total polymorphic percentage of 61.45%. The dendrogram based on 10 ISSR markers showed that the 23 chili genotypes were retrieved into three main groups (I, II, III, IV) with genetic similarity coefficient values ranging from 0.67 to 0.97 or a diversity of features of 0.03–0.33 (30%). Group I consisted of 1 genotype, group II consisted of 3 genotypes, and group III consisted of 14 genotypes. Kopay chili (G20) has a large genetic distance compared to the other genotypes, while Lolai and Gero chilies have a very high similarity. This genetic diversity information can be used as a basis for generating crosses between genotypes in a breeding program. The farther the genetic distance will produce a high population diversity, the greater the chance of getting superior hybrids.

Keywords: Capsicum annum, ISSR, local chili, West Sumatra

[1] Bosland PW, Votava EJ, Votava EM. Peppers: vegetable and spice capsicums. UK: Cabi; 2012. https://doi.org/10.1079/9781845938253.0000.

[2] Wang D, Bosland PW. The genes of Capsicum. HortScience. 2006;41(5):1169–87.

[3] Rahmah IN, Sulistyono A, Makhziah M. Growth response and yield of eggplant (Solanum melongena L.) to Paklobutrazol and water hyacinth liquid organic fertilizer. PLANTROPICA: Journal of Agricultural Science. 2021;6(2):154–62.

[4] Cho SY, Kim HW, Lee MK, Kim HJ, Kim JB, Choe JS, et al. Antioxidant and antiinflammatory activities in relation to the flavonoids composition of pepper (Capsicum annuum L.). Antioxidants. 2020 Oct;9(10):986.

[5] Suliansyah I, Ekawati F, Hariandi D. Identification and morphological characterization of local red chilies from West Sumatra. InIOP Conference Series: Earth and Environmental Science 2023 Apr 1, Vol. 1160, No. 1, p. 012013. England: IOP Publishing; 2023. https://doi.org/10.1088/1755-1315/1160/1/012013.

[6] Terryana RT, Nugroho K, Rijzaani H, Lestari P. Karakterisasi keragaman genetik 27 genotipe cabai berdasarkan marka SSR (Simple Sequence Repeat). Berita Biologi. 2018;17(2):183–94.

[7] da Costa FR, Pereira TN, Vitória AP, de Campos KP, Rodrigues R, da Silva DH, et al. Genetic diversity among Capsicum accessions using RAPD markers. Crop Breed Appl Biotechnol. 2006;6(1):18–23.

[8] Kang MS. Genotype-environment interaction and stability analyses: an update. Quantitative genetics, genomics and plant breeding. 2020;9:140-61. https://doi.org/10.1079/9781789240214.0140.

[9] Terryana RT, Ningrum ND, Nugroho K, Saptadi D, Kurniawan H, Lestari P. Analisis keragaman genetik dan pengembangan profil sidik jari DNA 20 varietas cabai lokal Indonesia berdasarkan marka SSR. Jurnal AgroBiogen. 2020;16(2):45–58.

[10] Bani PW, Daryono BS, Purnomo P. Penanda molekuler inter simple sequence repeat untuk menentukan ketahanan tanaman jagung terhadap penyakit bulai. J Fitopatologi. 2017;13(4):127–35.

[11] Hariyani SA, Zubaidah S. Molecular Characterization of Piper Retrofractum Vahl in Java using Inter Simple Sequence Repeats (ISSR) Markers. BIOEDUKASI. 2022;20(1):1–7.

[12] Olatunji TL, Afolayan AJ. Evaluation of genetic relationship among varieties of Capsicum annuum L. and Capsicum frutescens L. in West Africa using ISSR markers. Heliyon. 2019 May;5(5):e01700.

[13] Vo TB, Huynh K, Tran TB, Swee KY, Nguyen LH. Assessment of genetic diversity of chili rootstock using ISSR marker. CTU J Innov Sustain Dev. 2016;(03):7–13.

[14] Noli ZA, Labukti HV. Pengaruh Ekstrak Paku Resam (Gleichenia linearis) sebagai Biostimulan terhadap Pertumbuhan dan Hasil Cabai Keriting (Capsicum annum L.). Kultivar Kopay. Agro Bali: Agricultural Journal. 2022;5(3):492–7.