Published date: Mar 08 2023

Journal Title: KnE Medicine

Issue title: 2nd International Conference on Medical Health Science (2nd ICMEDH)

Pages: 403–409

DOI: 10.18502/kme.v3i2.13074

Desy Andaridesy_andari@umm.ac.idUniversity of Muhammadiyah Malang

Mutia Nur SalsabilaUniversity of Muhammadiyah Malang

Meddy SetiawanUniversity of Muhammadiyah Malang

Diah HermayantiUniversity of Muhammadiyah Malang

Dyslipidemia is a lipid fraction abnormality in the form of an increase in total cholesterol, LDL, and triglyceride levels and a decrease in HDL. Red Cabbage Aqueous Extract (RCE) contains antioxidants, one of which is anthocyanins, that can increase HDL levels. This research was conducted to determine the effect of RCE on HDL levels of white male rats induced by an atherogenic diet. True experimental with post-test only control group design with 15 rats divided into the standard group (KN: regular feed), negative control group (K-: atherogenic diet), and three treatment groups (P1: atherogenic-diet with RCE doses of 6.65 mg; P2: atherogenic-diet with RCE doses of 13,3 mg; and P3: atherogenic-diet with RCE doses of 26.6 mg/100grBW) was used. The HDL measurement method used spectrophotometry. Brassica oleracea L extract had no significant effect (Kruskal-Wallis test p = 0.465) on HDL levels of white male rats. This result also happened due to the low bioavailability of anthocyanins in aqueous extract. The linear regression test results showed that the relationship’s strength was 0.031 or very weak with a linear proportional correlation, increasing the dose and increasing HD levels. Based on the results of the tests, there was no significant effect of giving Red Cabbage (Brassica oleracea) Aqueous Extract on HDL levels of white male rats (Rattus norvegicus wistar strain), which was induced by an atherogenic diet.

Keywords: anthocyanins, atherogenic diet, HDL, red cabbage aquous extract

[1] Milutinović A, Šuput D, Zorc-Milutinović A, Šuput D, Zorc-Pleskovič R. 2020. Pathogenesis of atherosclerosis in the tunica intima, media, and adventitia of coronary arteries: An updated review. Bosnian Journal of Basic Medical Sciences. 2020;20(1):21–30. https://doi.org/10.17305/bjbms.2019.4320.P.

[2] Hasil Utama Riskesdas TI. Provinsi. Riskesdas. 2018;2018:52.

[3] Onwe P, Folawiyo M, Ogah A, Umahi G, Okorocha A, Afoke A. Hyperlipidemia: Etiology and Possible Control. IOSR J Dent Med Sci. 2015;14(10):2279–861.

[4] Brites F, Martin M, Guillas I, Kontush A. Antioxidative activity of high-density lipoprotein (HDL): mechanistic insights into potential clinical benefit. BBA Clin. 2017 Aug;8( July):66–77.

[5] Cruz AB, Pitz HD, Veber B, Bini LA, Maraschin M, Zeni AL. Assessment of bioactive metabolites and hypolipidemic effect of polyphenolic-rich red cabbage extract. Pharm Biol. 2016 Dec;54(12):3033–9.

[6] Qin Y, Xia M, Ma J, Hao Y, Liu J, Mou H, et al. Anthocyanin supplementation improves serum LDL- and HDL-cholesterol concentrations associated with the inhibition of cholesteryl ester transfer protein in dyslipidemic subjects. Am J Clin Nutr. 2009 Sep;90(3):485–92.

[7] El Mashad GM, ElSayed HM, Nosair NA. Effect of vitamin C supplementation on lipid profile, serum uric acid, and ascorbic acid in children on hemodialysis. Saudi J Kidney Dis Transpl. 2016;27(6):1148–54.

[8] Shalaby SM, Amin HH. Red Cabbage and Turmeric Extracts as Potential Natural Colors and Antioxidants Additives in Stirred Yogurt. J Probiotics Health. 2018;06(02). https://doi.org/10.4172/2329-8901.1000206.

[9] Kaseke MM, Tanudjaja GN, Panjaitan FW. Gambaran Histologik Aorta Tikus Wistar Dengan Diet. Univ Sam Ratulangi. 2014;2:76–82.

[10] Millar CL, Duclos Q, Blesso CN. Effects of dietary flavonoids on reverse cholesterol transport, HDL metabolism, and HDL function. Adv Nutr. 2017 Mar;8(2):226–39.

[11] Rye KA, Barter PJ. Regulation of high-density lipoprotein metabolism. Circ Res. 2014 Jan;114(1):143–56.

[12] Zhu Y, Huang X, Zhang Y, Wang Y, Liu Y, Sun R, et al. Anthocyanin supplementation improves HDL-associated paraoxonase 1 activity and enhances cholesterol efflux capacity in subjects with hypercholesterolemia. J Clin Endocrinol Metab. 2014 Feb;99(2):561–9.

[13] Kazeminasab F, Marandi M, Ghaedi K, Esfarjani F, Moshtaghian J. Effects of a 4- week aerobic exercise on lipid profile and expression of LXRα in rat liver. Cell J. 2017;19(1):45–9.

[14] Castañeda-Ovando A, Pacheco-Hernández ML, Páez-Hernández ME, Rodríguez JA, Galán-Vidal CA. Chemical studies of anthocyanins: A review. Food Chem. 2009;113(4):859–71.

[15] Khoo HE, Azlan A, Tang ST, Lim SM. Anthocyanidins and anthocyanins: colored pigments as food, pharmaceutical ingredients, and the potential health benefits [Internet]. Food Nutr Res. 2017 Aug;61(1):1361779.