Published date: Mar 08 2023

Journal Title: KnE Medicine

Issue title: 2nd International Conference on Medical Health Science (2nd ICMEDH)

Pages: 178–188

DOI: 10.18502/kme.v3i2.13050

Santika Tri WahyuniDepartment of Pharmacy, Faculty of Health Science, University of Muhammadiyah Malang, Indonesia

Dyah Rahmasaridyahrahmasari@umm.ac.idDepartment of Pharmacy, Faculty of Health Science, University of Muhammadiyah Malang, Indonesia

Rifqi Sandi NugrohoDepartment of Pharmacy, Faculty of Health Science, University of Muhammadiyah Malang, Indonesia

Ilham AgustaDepartment of Pharmacy, Faculty of Health Science, University of Muhammadiyah Malang, Indonesia

Revie Dithya Kurnia DamindaDepartment of Pharmacy, Faculty of Health Science, University of Muhammadiyah Malang, Indonesia

Riko Vikri SundugestiDepartment of Pharmacy, Faculty of Health Science, University of Muhammadiyah Malang, Indonesia

Dian ErmawatiDepartment of Pharmacy, Faculty of Health Science, University of Muhammadiyah Malang, Indonesia

Masks are personal protective equipment essential for COVID-19 prevention, but for the use of masks has led to an increase in the severity of both acne (maskne) and rosacea (mask rosacea). Long-time mask-wearing can increase acne’s flare by modifying the cutaneous facial microenvironment and triggering facial dermatoses. To minimize this, the skin needs to be protected by using an antibacterial product. This study aimed to develop and determine the antibacterial effect of Piper betle extract in niosome serum gel and its irritation effect. 5% Piper betle extract was formulated into a niosome system and incorporated into the serum gel with varying concentrations of 30%, 40%, and 50%. The niosome system was characterized by particle size, Polydispersity Index (PI), zeta potential, and pH value, and then evaluated physicochemical properties for niosome serum gel preparation. Further, the antibacterial effect against Propionibacterium acne, Staphylococcus aureus, and Staphylococcus epidermidis was tested using a well-diffusion test. The preparation stability was evaluated using freeze-thaw methods, and the irritation test was assessed using the HET-CAM (Hen’s Egg Chorioallantoic Membrane) method. The results showed that the preparation has a good physicochemical, while the best inhibition zone diameter was 7,29±0,21; 7,77±0,12; and 8,24±0,40, against P.acne, respectively. However, the results of the stability test of the preparation showed a significant change in the pH but had no irritation effect on CAM. It can concluded that the niosome serum gel of Piper betle extract has a potential antibacterial impact for acne, especially against P. acne.

Keywords: Piper betle, Niosome serum gel, antibacterial effect, stability, irritation effect

[1] Spigariolo CB, Giacalone S, Nazzaro G. Maskne: The Epidemic within the Pandemic: From Diagnosis to Therapy. J Clin Med. 2022;11(3):618.

[2] Teo WL. Diagnostic and Management Considerations for “‘Maskne”’ in The Era of COVID-19. J Am Acad Dermatol. 2021;84(2):520–1.

[3] Kosasih LP. MASKNE: Mask-Induced Acne Flare During Coronavirus Disease-19. What is it and How to Manage it? | Open Access Macedonian Journal of Medical Sciences. Open Access Maced J Med Sci. 2020;8 T1:411–5.

[4] Ermawati FU, Sari R, Putri NP, Rohmawati L, Kusumawati DH, Munasir, et al. Antimicrobial Activity Analysis of Piper betle Linn Leaves Extract from Nganjuk, Sidoarjo and Batu against Escherichia coli, Salmonella sp., Staphylococcus aureus and Pseudomonas aeruginosa. J Phys Conf Ser. 2021;1951(1):012004.

[5] Nayaka NM, Sasadara MM, Sanjaya DA, Yuda PE, Dewi NL, Cahyaningsih E, et al. Piper betle (L): Recent Review of Antibacterial and Antifungal Properties, Safety Profiles, and Commercial Applications. Molecules. 2021;26(8):1–21.

[6] Kurniasari SY, Tjahjaningsih W, Sianita N. Antibacterial Activity of Betel Leaf (Piper betle L.) Leaves Extract on Vibrio harveyi. IOP Conf Ser Earth Environ Sci. 2021;718(1):012048.

[7] Heliawati L, Lestari S, Hasanah U, Ajiati D, Kurnia D. Phytochemical Profile of Antibacterial Agents from Red Betel Leaf (Piper crocatum Ruiz and Pav) against Bacteria in Dental Caries. Molecules. 2022;27(9):2861.

[8] Meinisasti R, Muslim Z. Krisyanella, Sunita R. The Effectiveness Test of Betel Leaf Ethanol Extract Cream (Piper Betle Linn) Toward Propionibacterium acnes Bacterial Growth. Biosci Med J Biomed Transl Res. 2020;4(2):10–7.

[9] Florenly F, Novelya N, Janiar M, Miranda M, Hai LQPD, Quang PM. Nano-Green Betel Leaf Extracts (Piper betle L.) Inhibits the Growth of Streptococcus mutans and Staphylococcus aureus. e-GiGi. 2022;10(2):154.

[10] Khatun M, Hoque M. Extraction of Ethanol Soluble Compound from Betel Leaf for Antibacterial Activity. Bangladesh J Microbiol. 2021;38(1):15–9.

[11] Aher P, Dandgavhal K, Bhandari D, Saindane H, Deore N, Amrutkar S. Niosomes as a Potential Drug Delivery System. Int J Pharm Sci Rev Res. 2021;68(1):21–7.

[12] Purwanti T, Hariyadi DM, Silvia C, D V. Characterization and release of ibuprofen in proniosome system (Ibuprofen-span 60-cholesterol). Int J Drug Deliv Technol. 2018;8(2):103–6.

[13] Ge X, Wei M, He S, Yuan WE. Advances of non-ionic surfactant vesicles (niosomes) and their application in drug delivery. Pharmaceutics. 2019;11(2):55.

[14] Surini S, Mubarak H, Ramadon D. Cosmetic Serum Containing Grape (Vitis vinifera L .) Seed Extract Phytosome : Formulation and in vitro Penetration Study. 2018;10(2):51– 5.

[15] Nair AB, Chaudhary S, Shah H, Jacob S, Mewada V, Shinu P, et al. Intranasal Delivery of Darunavir-Loaded Mucoadhesive In Situ Gel: Experimental Design, In Vitro Evaluation, and Pharmacokinetic Studies. Gels. 2022;8(6):1–25.

[16] Khan R, Irchhaiya R. In Vitro in Vivo Evaluation of Niosomal Formulation of Famotidine. Int J Pharm Pharm Sci. 2020;12(3):15–22.

[17] Rahmasari D, Hendradi E, Chasanah U. Formulation and evaluation of hand sanitizer gel containing infused of binahong leaf (Anredera cordifolia) as antibacterial preparation. Farmasains. J Farm dan Ilmu Kesehat. 2020;5(1):23–30.

[18] Kumar A, Dua J. Formulation and Evaluation of Itraconazole Niosomal Gel. Asian J Pharm Res Dev. 2018;6(5):76–80.

[19] Luepke NP. Hen’s egg chorioallantoic membrane test for irritation potential. Food Chem Toxicol. 1985;23(2):287–91.

[20] Zhang G, Sun J. Lipid in Chips: A Brief Review of Liposomes Formation by Microfluidics. Int J Nanomedicine. 2021;16:7391–416.

[21] Danaei M, Dehghankhold M, Ataei S, Hasanzadeh Davarani F, Javanmard R, Dokhani A, et al. Impact of particle size and polydispersity index on the clinical applications of lipidic nanocarrier systems. Pharmaceutics. 2018;10(2):1–17.

[22] Patil VB, Tadavi SA, Pawar SP. Formulation of niosomal gel of aceclofenac and its in-vitro characterization. Int J Pharma Chem Res. 2017;3(3):467–73.

[23] Asthana GS, Sharma PK, Asthana A. In Vitro and in Vivo Evaluation of Niosomal Formulation for Controlled Delivery of Clarithromycin. Scientifica (Cairo). 2016;2016:1–10.

[24] Jaiswal PH, Gujarathi NA, Rane BR, Pawar SP. Formulation of Niosomal Gel of Diclofenac Sodium and Its In-vitro Characterization. Int J Pharm Pharm Res. 2016;6(4):585–600.

[25] Rahmawanty D, Yulianti N. Fitriana M. FORMULATION AND EVALUATION PEEL-OFF FACIAL MASK CONTAINING QUERCETIN WITH VARIATION CONCENTRATION OF GELATIN AND GLYCERIN. Media Farm. 2015;12(1):17–32.

[26] Deuschle VC, Deuschle RA, Bortoluzzi MR, Athayde ML. Physical chemistry evaluation of stability, spreadability, in vitro antioxidant, and photo-protective capacities of topical formulations containing calendula officinalis L. Leaf extract. Braz J Pharm Sci. 2015;51(1):63–75.

[27] Budiman A, Rusnawan DW, Yuliana A. Antibacterial Activity of Piper betle L. Extract in Cream Dosage Forms against Staphylococcus aureus and Propionibacterium acne. J Pharm Sci Res. 2018;10(3):493–6.

[28] Khafifa IN, Shabrina A, Rochman MF. Stability and Sunscreen Activity of Nutmeg Seed Oil Emulgel With Carbopol 940 Variation As Gel Base. J Farm Sains dan Prakt. 2022;8(2):167–76.