Published date:Sep 11 2017

Journal Title: KnE Life Sciences

Issue title: International Conference on Natural Resources and Life Sciences (NRLS-2016)

Pages:131-138

DOI: 10.18502/kls.v3i5.985

Liling Delila

Evi Susantievi.susanti.fmipa@um.ac.idDept. of Chemistry, Faculty of Science and Mathematics, Universitas Negeri Malang, Jalan Semarang No. 5, 65145, Malang, Indonesia

Eli Hendrik Sanjaya

The aim of this research is to obtain indigenous fungus producing lignin peroxidase from the Cocoa Plantation in Sepawon Kediri Regency, Indonesia. Isolation was done using Minimum Salt Medium alkali lignin (MSM - L), purification was performed using monospore technique, lignin peroxidase activity was screened by agar medium containing methylene blue, and microscopic morphology of fungi was studied using slide culture technique. Five isolates of fungus were obtained and three isolates of fungus with different morphology were purified. The screening results showed that only two fungus isolates were indicated as the indigenous fungus producing lignin peroxidase were KLUM₁ and KLUM₂. KLUM₁ was morphologycally predicted as Sporotricum aereum and KLUM₂ was predicted close to genus Crysosporium sp.

[1] Tien M, Kirk TK. 1983. Lignin degrading enzyme from hymenomycete Phanerochaete chrysosporium burds. Science, 221:661–663

[2] Chia CC. Enhanced production of lignin peroxidase and manganese peroxidase by Phanerochaete chrysosporium in a submerged culture fermentation and their application in decolourisation of dyes. [Thesis]. University of Science, Malaysia 2008.

[3] Hamid M, Khalil-ur-Rehman. Potential applications of peroxidase. Food Chemistry 2009;115:1177–1186.

[4] Mendonça Maciel MJ, Castro e Silva A, Ribeiro HCT. Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: A review. Electronic Journal of Biotechnology 2010;13(6):1–13.

[5] Paula B, Haim L, Carloz D. Use of lignin peroxidase in skin and hair lightening. European patent application. No EP2338896 A2 (2011) [Online] from https://www.google.com/patents/EP2338896A2?cl=en [Accessed on March 15th 2016]

[6] Supriyanto A. Manfaat jamur pelapuk putih Phaenerochaete chrysosporium L1 dan Pleurotus EB9 untuk biobleaching pulp kardus bekas [Biobleching of used cardboard pulp by White Rot Fungi Phanerochaete chrysosporium L1 and Pleurotus EB9]. [Thesis]. Institut Pertanian Bogor. 2009. [in Bahasa Indonesia].

[7] Nelson, Suparjo. Penentuan lama fermentasi kulit buah kakao dengan Phanerochaete chrysosporium: Evaluasi kualitas nutrisi secara kimiawi [Determination length of fermentation of cocoa pod husk with Phanerochaete chrysosporium: Chemically evaluation of nutrition quality]. [Thesis]. Universitas Jambi. 2011. [in
Bahasa Indonesia].

[8] Sasikumar V, Priya V, Shankar CS, Sekar SD. Isolation and preliminary screening of lignin degrading microbes. Journal of Academia and Industrial Research 2014;3(6): 291–294.

[9] Watanabe T. Pictorical atlas of soil and seed fungi: Morphologies of cultured fungi and key to species. 2ed ed. CRC Press LLC; 2002

[10] Campbell CK, Johnson EM, Warnock DW. Identification of pathogenic fungi. 2nd ed. New Jersey: Wiley Blackwell; 2013.

[11] EMSL Analytical, Inc. Sporotrichum. [Online] from http://www.emsl.com/Page .aspx?ID=262. [Accessed on 12 January 2017]

[12] Stalpers JA. Revision of the genus Sporotricum. Studies in Mycologi 1984; 24: 1–105.

[13] Susanti E, Ardyati T. Phylogenetic analysis of Phanerochaete chrysosporium ITB isolate using internal transcribed spacer (ITS) sequence. International Journal of ChemTech Research 2015;8(6): 654–658.

[14] Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, et al. NuclearV ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi. Proceedings of the National Academy of Sciences of the USA 2012;109(16):1–6.