Published date: Nov 25 2019

Journal Title: KnE Life Sciences

Issue title: International Scientific and Practical Conference “AgroSMART – Smart Solutions for Agriculture”

Pages: 578–588

DOI: 10.18502/kls.v4i14.5645

Flyura Alistratovaalistraatova@yandex.ruSaint-Petersburg State Academy of Veterinary Medicine, Saint-Petersburg, Russia

Toropova YanaBioprosthetics and Cardioprotection Research Lab, ”V.A. Almazov National Medical Research Center” Ministry of Health of the Russian Federation

Nika Bulavinovaocational Education Program, Pediatrics Occupation Studies Program, St. Petersburg State Pediatric Medical University, Saint-Petersburg, Russia

Ekaterina SmirnovaSaint-Petersburg State Academy of Veterinary Medicine, Saint-Petersburg, Russia

The work is devoted to the study of the effect of interval hypoxic training on the state of microcirculation of the skin in rats in the experiment. The study was conducted on white male Wistar rats weighing 70 g ±10%. In order to study the state of the dynamic characteristics of blood microcirculation, a change in blood perfusion of the tissue was considered. A non-invasive method for quantifying microvascular perfusion was used. The results showed that inhibition of microcirculation up to 50 % was observed in comparison to this value before exposure during the formation of a compensatory reaction of the body to a hypoxic effect on the 7–14th day of the experiment. It was noted that the predominant role in the regulation of the microvasculature belongs to the passive regulation factor of microcirculation which is characterized by the involvement of blood vessels in the microvasculature due to periodic changes in blood volume in the vessel together with an increase in the arithmetic mean perfusion rate. Thus, experimentally modulated hypobaric exposure, changes the volume of blood in arterioles that is determined by a pulse wave.

[1] Enikeev, D.A., Ryakhovsky, A.E., Fatkullin, K.V. et al. (2015). The dynamics of peripheral blood flow in rats with acute carbon monoxide poisoning according to laser Doppler flowmetry Modern problems.

[2] Krupatkin, A.I. (2014). Blood flow oscillations -- new diagnostic language in microvascular research. Regional hemodynamics and microcirculation, vol. 13(1), pp. 83–99.

[3] Félétou, M., Köhler, R., Vanhoutte, P.M. (2012). Nitric oxide: orchestrator of endothelium-dependent responses. Ann Med., vol. 44, no. 7, pp. 694–716.

[4] Serebrovskaya, T.V., Shatilo, V.B. (2014). The experience of using interval hypoxia for the prevention and treatment of diseases of the cardiovascular system. Overview. Zhurn. blood circulation and hemostasis, vol. 1–2, pp. 16–33

[5] Solovyova, A. G., Krylova E. V., Nepryakhina, I. I., Peretyagin, P. V. (2015). The state of microcirculation in rats with thermal injury under the influence of royal jelly. Bioradicals and antioxidants, vol. 2, no. 4, pp. 50--55.

[6] Shi, R., Chen, M., Tuchin, V.V. et al. (2015). Accessing to arteriovenous blood flow dynamics response using combined laser speckle contrast imaging and skin optical clearing, Biomed. Opt. Express, vol. 6(6), pp. 1977–1989.

[7] Jung, C., Jung, F., Kelm, M. (2016). The microcirculation in hypoxia: The center of the battlefield for oxygen Clinical Hemorheology and Microcirculation, vol. 63, no. 3, pp. 169–172.

[8] Benedikt, T., Kleinsasser A., Stadlbauer K. et al. (2018). Cutaneous Microvascular Blood Flow and Reactivity in Hypoxia Frontiers in Physiology, vol. 9 pp. 154–157.

[9] Paparde, A., Plakane, L., Circenis, K., Aivars, J.I. (2015). Effect of acute systemic hypoxia on human cutaneous microcirculation and endothelial, sympathetic and myogenic activity. Microvasc. Res. no. 102, pp. 1–5.

[10] Niedermeier, M., Weisleitner, A., Lamm, C., Ledochowski, L., Frühauf, A., Wille, M. et al. (2017b). Is decision making in hypoxia affected by pre-acclimatisation? A randomized controlled trial. Physiol. Behav, no. 173, pp. 236–242.

[11] Casey, D.P., Joyner, M.J. (2012). Compensatory vasodilatation during hypoxic exercise: mechanisms responsible for matching oxygen supply to demand. J. Physiol., no. 590, pp. 6321–6326.

[12] Baboshina, N.V. (2016). Evaluation of microcirculation in children of 8 and 10 years of age using inspiratory breath hold. Bulletin of Russian State Medical University, no. 3, pp. 51–56.

[13] Feng, W., Zhang, Ch., Yu, T., Zhu, D. (2019). Quantitative evaluation of skin disorders in type 1 diabetic mice by in vivo optical imaging, Biomed. Opt. Express, vol. 10, pp. 2996–3008.

[14] Feng, W., Shi, R., Zhang, Ch. et al. (2017). Lookup-table-based inverse model for mapping oxygen concentration of cutaneous microvessels using hyperspectral imaging. Opt. Express, vol. 25, pp. 3481–3495.

[15] Arutyunyan, G.A., Aliyev, A.R., Vlasov, K.E. et. al. (2018). Arterial infusion in the treatment of patients with acute pancreatitis. Bulletin of Medical Science, no. 2(10), pp. 52–55.

[16] Popovich, N.V., Raspopova, E.A. (2018). The role of breath holding at the initial stage of teaching children with mental disorders swimming. The Russian Journal of Physical Education and Sport., no. 13(4), pp. 39–42.

[17] Willis, S.J., Peyrard, Rupp, A.T. et al. (2019). Vascular and oxygenation responses of local ischemia and systemic hypoxia during arm cycling repeated sprints. Journal of Science and Medicine in Sport. DOI: 10.1016/j.jsams.2019.05.001.

[18] An X., Zhang H., Sun Y., and Ma X., The microcirculatory failure could not weaken the increase of systematic oxygen extraction rate in septic shock: An observational study in canine models. Clin Hemorheol Microcirc 63 (3) (2016), 267–279. DOI 10.3233/CH-152022.