Published date: Nov 25 2019

Journal Title: KnE Life Sciences

Issue title: International Scientific and Practical Conference “AgroSMART – Smart Solutions for Agriculture”

Pages: 478–487

DOI: 10.18502/kls.v4i14.5635

E V Vasnetsovams.eviv@mail.ruNational Research Saratov State University named after N.G. Chernyshevskogo, Saratov, Russia

E I TikhomirovaYuri Gagarin State Technical University of Saratov, Saratov, Russia

O Yu KsenofontovaNational Research Saratov State University named after N.G. Chernyshevskogo, Saratov, Russia

S Yu EfremovaPenza State Technological University, Penza, Russia

A promising approach for remediation of contaminated soils is the use of biological products based on microorganisms-destructors. Of particular interest are the strains that can decompose several types of pollutants. Potential strains-destructors should be not only effective but also adapted for certain soil and climatic conditions. In addition, when introducing a significant amount of biological agents into the ecosystem, the lack of toxicological and pathogenic effects on plants, animals, and humans is particularly important. The object of the study is the destructor strain of sim-triazine herbicide of prometrin Pseudomonas putida P2. The study explores the ability of strain to use various pesticides as the only carbon source, the effect of bacterial culture on seed germination, biotesting of strain on toxicity using earthworms. Besides, the study presents the phytopathogenic factors and enzymes causing bacteria invasiveness into animals and humans. The determination of dehydrogenase activity of prometrin destructor strain shows that this strain can also use active ingredients of pesticide malathion, HCH, and 4,4-DDT as only sole carbon source. It is also established that the studied strain Pseudomonas putida P2 increases the germination of seeds of the variety Saratovskaya 70 and carrots of the variety Samson and does not possess pathogenicity factors. The strain biotesting on the earthworms revealed no acute toxicity of the culture. The analysis of data obtained from the model pollution shows that the introduction of the destructor of Prometrium Pseudomonas putida P2 into the soil can create favorable conditions for vital activity and reproduction of earthworms.

[1] Turkovskaya, O.V., Panchenko, L.V., Dubrovskaya E.V. et al. (2003). Biotechnology for environmental cleanup. Coll. scientific works ``High Technologies -- Path to Progress''. Saratov: Scientific Book, pp. 76–81.

[2] Gorbatenko, N.O. (2006). Pesticides: structure and degradation processes. Successes of Biological Chemistry, vol. 4, pp. 323–348.

[3] Turkovskaya, O.V., Dubrovskaya, E.V., Muratova A.Yu. et al. (2002). Possibilities of modern methods of environmental bioremediation. Proceedings of the 1stInternational Congress ``Biotechnology -- state and development prospects'', p. 290. Moscow.

[4] Ivlev, A.M., Derbentseva, A.M. (2003). Soil degradation and their recultivation. Vladivostok: FESU Publishing House, 86 p.

[5] Pleshakova, E.V. (2010). Ecological and functional aspects of microbial remediation of oil-contaminated soils. PhD dissertation thesis. Saratov, 42 p.

[6] Koshelev, A.V., Golovkov, V.F., Tikhomirova, E.I., Ksenofontova, O. Yu. (2017). Questions of modern science and practice. University named after V.I. Vernadsky, no. 3(65). DOI 10.17277 /voprosy.217.03. pp. 040–050.

[7] Reshetov, G.G., Belov, V.S., Korsak, V.V. (2008). Soils of Saratov region. Saratov: Saratov State Social and Economic University, 180 p.

[8] Kolesnikov, S.I., Kaseev, K.Sh., Valkov, V.F. (2006). Ecological state and functions of soils in the conditions of chemical pollution. Rostov-on-Don: Rostizdat, 385 p.

[9] Oliskevich, V.V., Talalovskaya, N.M., Tikhomirova, E.I., Ksenofontova, O.Yu., Tretyakova, S.E. (2018). The experience of creating biological products based on biodestructors of pesticides and testing their effectiveness on experimentally contaminated soil systems. Materials of IV Intern. conf. ``Actual scientific and scientific and technical problems of ensuring chemical safety'', p. 110. Moscow: Publishing House of the Russian Academy of Sciences.

[10] Korolev, V.A. (2001). Cleaning soils from pollution. Moscow: Agro, 365 p.

[11] Ksenofontova, O.Yu., Tretyakov, S.E., Tikhomirova, E.I., Vasnetsov, E.V. (2016). Technology development for the use of biological product for the remediation of soils contaminated with pesticide prometrin in laboratory and field conditions. Proceedings of Samara Scientific Center of the Russian Academy of Sciences, vol. 18, no. 2–3, pp. 718–723.

[12] Granatskaya, T.A., Dvornikova, T.P. (1996). Method for detection of xenobiotic destructive microorganisms Pat. no. 2051961, Russian Federation, IPC, Section C C12 C12Q C12Q 1/00 Measurement or testing using enzymes or microorganisms; formulations; methods of obtaining such compositions. Date of patent publication: 01.10.1996.

[13] Zheldakova, R.A., Myamin, V.E. (2006). Phytopathogenic microorganisms. Study guide for students of Biology Department. Minsk: BSU, 116 p.

[14] Netrusov A.I. et al. (2005). Case study on Microbiology. Study Book for HEI students. Moscow: Publishing Center Academy, 608 p.

[15] GOST 12038-84. (2011). Crop seeds. Methods for determining germination. Moscow: Standardinform, 30 p.

[16] International Standard ISO 11268-1 Determination of pollution from acute lethal toxicity in earthworms.