KnE Life Sciences
ISSN: 2413-0877
The latest conference proceedings on life sciences, medicine and pharmacology.
About the Applicability of Bone and Cartilage Acid-free Staining for Anura
Published date: May 03 2018
Journal Title: KnE Life Sciences
Issue title: The Second International Conference on Amphibian and Reptiles Anomalies and Pathology
Pages: 156–160
Authors:
Abstract:
The article describes the first use of acid-free alcian-alizarin staining for tailless amphibians in comparison with the standard method of acid staining on the example of the moor frog. The acid-free protocol is applicable for Anura and it has a number of advantages, such as the absence of decalcification, safety for the researcher and
others. However, this method also has limitations, such as the binding of alcian blue with soft tissues.
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