Published date: Oct 04 2024

Journal Title: KnE Life Sciences

Issue title: 4th International Conference in Social Science (4th ICONISS): Healthcare

Pages: 111–122

DOI: 10.18502/kls.v8i2.17364

Sayu Putu Yuni ParyatiDepartment of Microbiology, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi

Damarjati NugrohoDepartment of Microbiology, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi

Khomaini Hasank.hasan@lecture.unjani.ac.idDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi

Rabies is a lethal viral animal disease that assaults the central nervous system. Its glycoprotein is a viral protein that is essential for viral pathogenicity. Initially, the rabies vaccine was produced from nerve tissue, but it is no longer recommended since it causes adverse effects and is less effective. The anti-idiotype antibody vaccination is one option that functions as homologous artificial antigens to the glycoprotein of the rabies virus. The CDR is the structure of anti-idiotypic antibodies that play a role in mimicking epitopes. It may resemble or be identical to epitopes seen in rabies virus glycoproteins. The objective of this study is to determine the affinity of the CDR anti-idiotype antibody for the rabies virus glycoprotein epitope by studying the CDR electrostatic value. This electrostatic value was analyzed by bioinformatics approaches using a webPIPSA server. The findings revealed an electrostatic resemblance between the structure of anti-idiotype antibodies and the rabies virus glycoprotein. Further study will be aimed at collecting electrostatic values from each structure to create an anti-rabies vaccine.

Keywords: anti-idiotype antibodies, CDR, electrostatic, rabies, glycoprotein, webPIPSA

[1] Bano I, Sajjad H, Shah AM, Leghari A, Mirbahar KH, Shams S, et al. A Review of Rabies Disease, its Transmission and Treatment. Journal of Animal Health and Production. 2016;4(4):140–4.

[2] Yousaf MZ, Qasim M, Zia S. Rehman Khan M ur, Ashfaq UA, Khan S. Rabies molecular virology, diagnosis, prevention and treatment. Virol J. 2012;:9.

[3] Nasional M. Masterplan Nasional Pemberantasan Rabies di Indonesia, Direktorat Jenderal Peternakan dan Kesehatan Hewan, Departemen Pertanian Republik Indonesia.

[4] Gautret P, Angelo KM, Asgeirsson H, Lalloo DG, Shaw M, Schwartz E, et al.; GeoSentinel Global Surveillance Network. Rabies post-exposure prophylaxis started during or after travel: A GeoSentinel analysis. PLoS Negl Trop Dis. 2018 Nov;12(11):e0006951.

[5] CDC. Ncird. National Center for Emerging and Zoonotic Infectious Diseases Rabies Pre-exposure Prophylaxis and Children Advisory Committee on Immunization Practices Meeting. 2021.

[6] World Health Organization. WHO Expert Consultation on Rabies : third report. n.d.

[7] Antia R, Ahmed H, Bull JJ. Directed attenuation to enhance vaccine immunity. PLOS Comput Biol. 2021 Feb;17(2):e1008602.

[8] Naveed A, Rahman SU, Arshad MI, Aslam B. Recapitulation of the anti-Idiotype antibodies as vaccine candidate. Transl Med Commun. 2018;3(1):3.

[9] Vázquez AM, Rodrèguez-Zhurbenko N, López AM. Anti-ganglioside anti-idiotypic vaccination: more than molecular mimicry. Front Oncol. 2012 Nov;2:170.

[10] Polonelli L, Pontón J, Elguezabal N, Moragues MD, Casoli C, Pilotti E, et al. Antibody complementarity-determining regions (CDRs) can display differential antimicrobial, antiviral and antitumor activities. PLoS One. 2008 Jun;3(6):e2371.

[11] Amro WA, Al-Qaisi W, Al-Razem F. Production and purification of IgY antibodies from chicken egg yolk. J Genet Eng Biotechnol. 2018 Jun;16(1):99–103.

[12] Pereira EP, van Tilburg MF, Florean EO, Guedes MI. Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review. Int Immunopharmacol. 2019 Aug;73:293–303.

[13] Nascimento IP, Leite LC. Recombinant vaccines and the development of new vaccine strategies. Braz J Med Biol Res. 2012 Dec;45(12):1102–11.

[14] Suza W, Lee D. GENETICS, AGRICULTURE, AND BIOTECHNOLOGY. [Internet]. Available from: https://LibreTexts.org

[15] Pan SY, Chia YC, Yee HR, Fang Cheng AY, Anjum CE, Kenisi Y, et al. Immunomodulatory potential of anti-idiotypic antibodies for the treatment of autoimmune diseases. Future Sci OA. 2021;7(2):7.

[16] Farld NR, Linthicum DS. Idiotypes, Paratopes, and Molecular Mimicry. D. S. Linthicum et al. (eds.), Anti-Idiotypes, Receptors, and Molecular Mimicry © Springer-Verlag New York Inc. 1988.

[17] Paryati SP, Hasan K, Nawangsih EN, Rakhmat II. Purification and characterization of rabies anti-idiotype antibodies isolated from chicken egg yolks. International Journal of Pharmaceutical Research. 2020;12:1629–34.

[18] Dassault Systemes BI. Discovery Studio 2019. San Diego: dassault Systemes 2019.Laskowski RA, MacArthur MW, Moss DS, Thornton JM. PROCHECK-a program to check the stereochemical quality of protein structures. J Appl Cryst. 1993;26:283– 91.

[19] Altschup SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic Local Alignment Search Tool. Volume 215. 1990.

[20] Richter S, Wenzel A, Stein M, Gabdoulline RR, Wade RC. webPIPSA: a web server for the comparison of protein interaction properties. Nucleic Acids Res. 2008 Jul;36(Web Server issue):W276-80.

[21] Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, et al. The Protein Data Bank. vol. 28. 2000.