KnE Life Sciences
ISSN: 2413-0877
The latest conference proceedings on life sciences, medicine and pharmacology.
Antiviral Activity Effect of Silver Nanoparticles (Agnps) Solution Against the Growth of Infectious Bursal Disease Virus on Embryonated Chicken Eggs with Elisa Test
Published date: Dec 03 2017
Journal Title: KnE Life Sciences
Issue title: The Veterinary Medicine International Conference (VMIC)
Pages: 536-548
Authors:
Abstract:
Infectious bursal disease virus is one of the strategic infectious disease in Indonesia. Despite disinfection and vaccination technology has been doing, the cases still frequently occur and it needs another alternative technology to be developed to against IBD virus. This research try to answer the problem, it examines the effect of antiviral activity of silver nanoparticles (AgNPs) solution against the growth of infectious bursal disease virus in embryonated chicken eggs with ELISA Test. The research has two methods, the first method is conducted by mixing a solution of AgNPs and IBD Virus, two hours before inoculated (preventive method) and the second method is the virus inoculated first, 48 hours later the AgNPs solution injected (therapy method). Each method has several dosage of AgNPs solution respectively 0 ppm (positive control 20 ppm, 40 ppm, and 50 ppm). Virus samples taken from the choriallantoic membrane (CAM) and the embryo by crushed method. Results based on the value of OD (optical density) ELISA Test and Statistical Test ANOVA General Linier Models Univariate with Post-Hoc Duncan 5%, both methods have no significant difference (p>0.05), it means the solution of AgNPs has good preventative and therapeutic characteristic. The mean of OD values also showed dosage of 20ppm is most effective dosage in against the growth of the virus, the dosage has significant difference (p<0.05). The decreasing amount of virus in CAM and in embryos were not significantly different (p>0.05), in both CAM and embryo AgNPs solution has good antiviral properties.
Keywords : Silver Nanoparticles, Antiviral, Infectious Bursal Disesase, ELISA, Embryonated Chicken Eggs
References:
Abidin, Zainal. 2002. Meningkatkan Produktivitas Ayam Ras Pedaging. Agro Media Pustaka.
AP 9080.08. Depok.
Abdou, H.M. 1989. Dissoution, Bioavailability and Bioequivalence. Easton, Pennsylvania: Mack Printing.
Adelwahab, K.S. and Z.N. Said. 2013. Antiviral Replication Agents. ISBN 978-953-51-1055-2.
DOI: 10.5772/53707. Cairo: German Rosas-Acosta.
Akoso, B.T. 1993. Manual Kesehatan Unggas. Yokyakarta: Penerbit Kanisius. 107-109.
Amin, A.K. 2013. Sintesis, Studi Kestabilan Dan Karakterisasi Koloid Ag Nanopartikel Dengan Reduktor Hidrazin Sulfat Dalam Media Kitosan. Yogyakarta: Universitas Gajah Mada.
Apriandanu, D.O., S. Wahyuni dan S.H. Haryono.. 2013. Sintesis nanopartikel perak menggunakan metode poliol dengan agen stabilisator polivinil alcohol. Jurnal MIPA 36(1) : 157-168 (2013) : Unnes. Semarang.
Ariyanta, K.A., S. Wahyuni dan Priatmoko. 2013. Preparasi nanopartikel perak dengan metode reduksi dan aplikasinya sebagai anti-bakteri penyebab infeksi. Indo J Sci Unnes. Semarang Asraf, S. 2005. Studies On Infectious Bursal Disease Virus. Disertasi.The Ohio University. Ohio.
Bahri, S., E. Masbulan dan A. Kusumaningsih. 2005. Proses praproduksi sebagai faktor penting dalam menghasilkan produk ternak yang aman untuk manusia. J. Litbang Pertanian 24:27-35.
Benton, W.J., M.S. Cover, J.K. Rosenberger and R.S. Lake. 1967. Physicochemical Properties of Infectious Bursal Agent of Chickens. Avian Dis. 11:438-439.
Budinuryanto, D.C., M.H. Hadiana, R.L. Balia, Abubakar dan E. Widosari. 2000. Profil keamanan daging ayam local yang dipotong di pasar tradisional dalam kaitannya dengan penerapan system HACCP. Laporan hasil penelitian lembaga penelitian Universitas Padjajaran di ARMP II Badan Penelitian dan Pengembangan Pertanian. Bandung.
Butcher, G.D. and R.D. Milles. 2003. Infectious Bursa Disease (Gumboro) In Commercial Broilers.
Castro, Eduardo A. and Haghi A.K. (2012). Nanoscience and Advancing Computational Methods in Chemistry : Research Progress. Canada-Argentina. IGI Global. Pg 170.
Chwalibog, A., E. Sawosz, T. Niemiec and M. Grodzik, 2009. Nanotechnology in poultry production. Can silver nanoparticles promote health and growth of chicken?. Midterm Research Report.
Cunningham, C. H. (1973). A laboratory guide in virology. Burgers publishing Co. Minnesota.
7th Edn. P155-169.
Delgado, C., M. Rosegrant, Steinfeld, Ehmis and C. Curbois. 1999. Livestock to 2020 : The Next
Revolution. Washington – Rome – Nairubi : IFPRI – FAO – ILRI.
Ditjenak (Direktorat Jendral Peternakan). 2006. Statistic Peternakan Tahun 2005. Jakarta:
Ditjenak.
Elechiguerra, JL., Burt JL., Morones JR., Camacho-Bragado A., Gao X., Lara HH., and Yacaman M.J. 2005. Interaction of Silver Nanoparticles with HIV-1. Texas-Mexico. Journal of Nanobiotechnology-BioMed Central.
Ernawati, R., A.P. Rahardjo, N.W. Sianita, J. Rahmahani, F.A. Rantam dan Suwarno. 2013. Petunjuk Praktikum: Pemeriksaan Virologik dan Serologik. Surabaya: Laboratorium Virologi dan Imunologi Departemen Mikrobiologi Fakultas Kedokteran Hewan Universitas Airlangga.
Fitrianingsih, R. 2012. Synthesis of Silver Nanoparticles by Chemical Reduction at Various Fraction of MSA and Their Structure Characterization. Malang. Academic Journal. AIP Conference Proceedings;2014, Vol. 1589, p257
Fransisca, 2012. Pengembangan prototype vaksin TB tahap identifikasi isolate M. tuberculosis, penyiapan antigen, uji imunogenitas dan pembuatan isolate M. tuberculosis, “dorman” in vitro. Jakarta. LAK: Badan Penelitian dan Pengembangan Kesehatan Tahun 2012.
Gaikward, S. Ingie A. and Galdiero M. 2013. Antiviral Activity of Mycosynthesized Silver Nanoparticles Against Herpes Simplex Virus and Human Parainfluenza Virus Type 3. International Journal of Nanomedicine. 2013;8;4303-4314. Doi:10.2147/IJN.S50070.
Galdiero, S., Falanga A., Vitiello M., Cantisani M., Marra V. and Galdiero M.. 2011. Silver nanoparticles as potential antiviral agents. Molecules. 16, 8894-8918. Naples.
Hirai, K. and S. Shimakura. 1974. Structure Of Infectious Bursal Disease Virus. J. Virol.14: 957
– 964.
Lara, H.H., L. Ixtepan-Turrent, E.N. Garza-Trevino and C. Rodriguez-Padilla. 2010. PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture. Journal of Nanobiotechnologi. San Nicolas de los Garza.
Lara, H.H., E.N. Garza-Trevino, L. Ixtepan-Turrent and D.K. Singh. 2011. Silver nanoparticles are broad-spectrum bacterial an virucidal compounds. singhd@wssu.edu. San Nicolas de los Garza.
Li, W.R., Y. Zhang, X. Yang, N. Chen, Y. Sun, Y. Zhao, C. Fan, Q. Huang, Y. Zhu and J. Li. Appl Microbiol Biotechnol. 2010, 85 (4), 1115-1122. Shanghai: China
Lubick, N. Nanosilver toxicity: ions, nanoparticles--or both? Environ. Sci. Technol., 2008, 42 (23), p 8617
Lukert, P.D. and Y.M. Saif. 2003. Infectious Bursal Disease. In: Diseases Of Poultry 11ThEd.
Saif, Y.M., H.J. Barnes And J.R. Glisson (Eds.). Iowa State University Press. Ames. Pp.
161 – 170.
Machdum, Nurvidia. 2009. Gumboro pada Ayam. Jakarta: Invovet.
Mehrasa, M., S.R. Zaker, B. Larki, M. mehmandoust, H. Baghshahi, M.H. Sekhavati and S. Gavanji. 2014. Computational Prediction and Analysis of Ineraction of Silver Nitrate with Peptidoglycans Associated lipoprotein (Pal). Rangpur. J Sci Res Env Sci. 2014, 2(1), 8-13.
Moura, L., V. Vakharia, M. Li and H. Song. 2007. In Ovo Vaccine Against Infectious Bursal Disease. Maryland. Int. J. Poult. Sci. 6: 770 – 775
Natte, S. and Natalie D. 2011 In-Vitro-In-Vivo Correlation Definition and Regulatory Guidance.
Baltimore. International Journal of Generic Drugs.
Oldenburg. S.J. 2012. Silver Nanomaterials: Properties and Applications. nanoComposix, Inc.
San Diego.
Pal, J. and M.K. Deb. 2012. Microwave synthesis of polymercoated silver nanoparticles by glucose as reducing agent. Indian J Chem Sci A 51 : 821-824. Chhattisgarh.
Partadiredja, M., W. Rumawas dan I. Suharyanto. 1983. Penyakit Gumboro Di Indonesia Dan Akibatnya Bagi Peternak Ayam. Hemerazoa 71(1): 29 – 33. Bogor.
Pedersen, Gordon. 2014. Preventing Viral Infection with Silver Nanoparticles. Salt Lake City: A New Fighting Chance: Silver Solution: A Quantum Leap In Silver Technology: How molecular structuring safely destroys bacteria, viruses and yeast.
Rahardjo, A.P. dan Suwarno. 2005. Imunogenesitas Protein VP2 Virus Infectious Bursal Disease Isolat Lokal sebagai Dasar Pengembangan Vaksin Sub Unit. Surabaya: Media Kedokteran Hewan Vol. 21 No. 1.
Riady, M. 2005. Upaya pengembangan industry peternakan nasional bebas dari penyakit-penyait strategis. Pros. Seminar Nasional Teknologi Peternakan dan Veteriner. Bogor : 12-13 September 2005. Puslitbang Peternakan, hlm : 3-9.
Rogers, J.V., Parkinson C.V., Choi Y.W., Speshock J.L. and Hussain S.M.. 2008. A Pleminiary Assessment of Silver Nanoparticle Inhibition of Monkeypox Virus Plaque Formation. Nanoscale Research Letters. Columbus.
Rosenberger, J.K., Y.M. Saif and D.J. Jackwood. 2008. Infectious Bursal Disease. In: Dufour-Zavala, L, Swayne, DE, Glisson, J. RJ, Pearson, E, Reed, WM, Jackwood, MW and Woolcock, PR, editors. A laboratory manual for the isolation and identification of avian pathogens 5th ed, American Association of Avian Pathologists; p. 188-190. Newark.
Saputra, A H., A. Haryono, J.A. Laksmono dan M.H. Anshari. 2010. Preparasi koloid nanosilver dengan berbagai jenis reductor sebagai bahan antibakteri. Depok : Jurnal Sains Materi Indonesia.
Suwarno, F.A. Rantam, R. Ernawati., N.W. Sianita, A.P. Rahardjo dan J. Rahmahani. 2010. ELISA. Teori dan Protokol. Departemen Mikrobiologi Fakultas Kedokteran Hewan Universitas Airlangga. Surabaya.
Tabbu, C. R. 2000. Penyakit ayam dan penanggulangannya. Penyakit bacterial, mikal dan viral Volume I. Yogyakarta: Penerbit Kanisius.
Tangenjaya, B. dan A. Djajanegara. 2002. Peternakan Indonesia tahun 2020 : Suatu Visi Agriculture and Rural Development Strategy Studi. ADB-3843. Bogor.
Van den berg, T.P. 2000 Infectious bursal disease (Gumboro Disease). Belgium: Rev Sci Tech Off Int Epiz 2000, 19(2), 527-543. Bruxelles, Belgium-Ploufragan, France.
Vindevogel, H., M. Gouffaux, G. Meulemans, J.P. Duchatel and P. Halen. 1976. Infectious bursal disease : distribution and persistence of the virus in inoculated chickens. Study on the transmission of the disease. Bruxelles.
Wahyuwardani, S., D.R. Agungpriyono, L. Parede dan W. Manalu. 2011. Penyakit Gumboro: Etiologi, Epidemiologi, Patologi, Diagnosis dan Pengendaliannya. Wartazoa vol. 21 No.3. Bogor.
Xiang, D.X., Q. Chen, L. Pang and C.L. Zheng. 2013. 2011. Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro. J Virol Methods. 2011;178(1–2):137– 142.
Xiang, D.X., Y. Zheng, W. Duan, X. Li, J. Yin, S. Shigdar, M.L. O’Connor, M. Marappan, X. Zhao, Y. Miao, B. Xiang and C.L. Zheng. 2013. Inhibition of A/Human/Hubei/3/2005 (H3N2) influenza virus infection by silver nanoparticles in
23
Annuja Anandaradje, Vadivel Meyappan, Indramani Kumar, Natarajan Sakthivel (2020)
Microbial Synthesis of Silver Nanoparticles and Their Biological Potential,First Page: 99
10.1007/978-981-13-8954-2_4
Helena I. O. Gomes, Catarina S. M. Martins, João A. V. Prior (2021)
Silver Nanoparticles as Carriers of Anticancer Drugs for Efficient Target Treatment of Cancer Cells, NanomaterialsVolume: 11, Issue: 4, First Page: 964
10.3390/nano11040964
Mina Zare, Vinoy Thomas, Seeram Ramakrishna (2021)
Nanoscience and quantum science-led biocidal and antiviral strategies, Journal of Materials Chemistry BVolume: 9, Issue: 36, First Page: 7328
10.1039/D0TB02639E
L. V. Shevchenko, Y. Y. Dovbnia, N. М. Permyakova, Т. B. Zheltonozhskaya, S. V. Shulyak, D. O. Klymchuk (2022)
Influence of nanosilver in hybrid carriers on morphological and biochemical blood pa-rameters of laying hens , Regulatory Mechanisms in BiosystemsVolume: 13, Issue: 1, First Page: 15
10.15421/022203
Avinash P. Ingle, Aniket Gade, Shital Bonde, Magdalena Wypij, Patrycja Golinska, Mahendra Rai (2023)
Nanotechnological strategies for drug delivery and treatment of COVID-19,First Page: 301
10.1016/B978-0-323-91763-6.00013-8
Thibault Frippiat, Tatiana Art, Catherine Delguste (2025)
Silver Nanoparticles as Antimicrobial Agents in Veterinary Medicine: Current Applications and Future Perspectives, NanomaterialsVolume: 15, Issue: 3, First Page: 202
10.3390/nano15030202
P.C. Sharma, D. Sharma, A. Sharma, N. Saini, R. Goyal, M. Ola, R. Chawla, V.K. Thakur (2020)
Hydrazone comprising compounds as promising anti-infective agents: chemistry and structure-property relationship, Materials Today ChemistryVolume: 18, First Page: 100349
10.1016/j.mtchem.2020.100349
Bhaskar Ganguly, Ashwini Kumar Verma, Balwinder Singh, Arup Kumar Das, Sunil Kumar Rastogi, Alireza Seidavi, Diamanto Lazari, Ilias Giannenas (2023)
Green Synthesis of Silver Nanoparticles with Extract of Indian Ginseng and In Vitro Inhibitory Activity against Infectious Bursal Disease Virus, PoultryVolume: 2, Issue: 1, First Page: 12
10.3390/poultry2010002
Mohammed Saleh, Ahmed El-Moghazy, Adel H. Elgohary, WesamEldin I. A. Saber, Yosra A. Helmy (2025)
Revolutionizing Nanovaccines: A New Era of Immunization, VaccinesVolume: 13, Issue: 2, First Page: 126
10.3390/vaccines13020126
Zunjiang Zhao, Xuan Zhang, Dalun Lv, Lei Chen, Baode Zhang, Dejin Wu (2023)
Fabrication of silver nanoparticles immobilized on magnetic lignosulfonate: Evaluation of its catalytic activity in the N-acetylation reactions and investigation of its anti-cutaneous squamous cell carcinoma effects, International Journal of Biological MacromoleculesVolume: 250, First Page: 125901
10.1016/j.ijbiomac.2023.125901
Fayiz M. Reda, Abdullah S. Alawam, Hemat K. Mahmoud, Mohamed T. El‐Saadony, Ayman S. Salah, Hassan A. Rudayni, Ahmed A. Allam, Karima El‐Naggar, Mahmoud Alagawany, Most Khairunnesa (2025)
Application of Green Cobalt Nanoparticles in the Diet of Broiler Chickens to Improve Sustainable Production and Health, Veterinary Medicine and ScienceVolume: 11, Issue: 4
10.1002/vms3.70508
Jaison Jeevanandam, Saravanan Krishnan, Yiik Siang Hii, Sharadwata Pan, Yen San Chan, Caleb Acquah, Michael K. Danquah, João Rodrigues (2022)
Synthesis approach-dependent antiviral properties of silver nanoparticles and nanocomposites, Journal of Nanostructure in ChemistryVolume: 12, Issue: 5, First Page: 809
10.1007/s40097-021-00465-y
Mindaugas Ilickas, Asta Guobienė, Karolis Gedvilas, Mantvydas Merkis, Brigita Abakevičienė (2024)
UV-mediated photochemical synthesis and investigation of the antiviral properties of Silver nanoparticle-polyvinyl butyral nanocomposite coatings as a novel antiviral material with high stability and activity, Applied Materials TodayVolume: 38, First Page: 102203
10.1016/j.apmt.2024.102203
Tran Thi Ngoc Dung, Vu Nang Nam, Tran Thi Nhan, Trinh Thi Bich Ngoc, Luu Quang Minh, Bui Thi To Nga, Van Phan Le, Dang Viet Quang (2020)
Silver nanoparticles as potential antiviral agents against African swine fever virus, Materials Research ExpressVolume: 6, Issue: 12, First Page: 1250g9
10.1088/2053-1591/ab6ad8
Hay Man Saung Hnin Soe, Thorsteinn Loftsson, Phatsawee Jansook (2024)
The application of cyclodextrins in drug solubilization and stabilization of nanoparticles for drug delivery and biomedical applications, International Journal of PharmaceuticsVolume: 666, First Page: 124787
10.1016/j.ijpharm.2024.124787
A. Balakrishnan, M. M. Jacob, P. Senthil Kumar, A. Kapoor, M. Ponnuchamy, P. Sivaraman, M. Sillanpää (2023)
Strategies for safe management of hospital wastewater during the COVID-19 pandemic, International Journal of Environmental Science and TechnologyVolume: 20, Issue: 12, First Page: 13941
10.1007/s13762-023-04803-1
Lukumoni Buragohain, Bhabesh Mili, Arpita Bharali, Anamika Mazumdar (2024)
Nanotherapeutics for Viral Diseases of Animals,First Page: 467
10.1007/978-981-16-1610-5_20
Mohamed Fawzy, Gasser M. Khairy, Ahmed Hesham, Ali A. Rabaan, Ahmed G. El-Shamy, Abdou Nagy (2021)
Nanoparticles as a novel and promising antiviral platform in veterinary medicine, Archives of VirologyVolume: 166, Issue: 10, First Page: 2673
10.1007/s00705-021-05177-w
Angelica Luceri, Rachele Francese, David Lembo, Monica Ferraris, Cristina Balagna (2023)
Silver Nanoparticles: Review of Antiviral Properties, Mechanism of Action and Applications, MicroorganismsVolume: 11, Issue: 3, First Page: 629
10.3390/microorganisms11030629
Samira Ranjbar, Ameneh Bakhtiari, Negin Khosravi, Shima Jafari Ashkavandi, Fariba Azamian, Mahfam Alijaniha , Mojtaba Karbalaee (2024)
Silver Nanoparticles: Biomedical Applications and Future Perspectives , Journal of Composites and CompoundsVolume: 6, Issue: 20
10.61186/jcc.6.3.2
Şevket Evci (2024)
Uses of nanotechnological feed additives and nanofeeds in poultry feeding, Tavukçuluk Araştırma DergisiVolume: 21, Issue: 1, First Page: 27
10.34233/jpr.1507538
Magdalena Skonieczna, Dorota Hudy (2018)
Biological Activity of Silver Nanoparticles and Their Applications in Anticancer Therapy,
10.5772/intechopen.77075
M.I. Anwar, M.M. Awais, M. Akhtar, M.T. Navid, F. Muhammad (2019)
Nutritional and immunological effects of nano-particles in commercial poultry birds, World's Poultry Science JournalVolume: 75, Issue: 2, First Page: 261
10.1017/S0043933919000199
