Published date: Jun 07 2022

Journal Title: KnE Life Sciences

Issue title: The First Asian PGPR Indonesian Chapter International e-Conference 2021

Pages: 664–671

DOI: 10.18502/kls.v7i3.11170

Yulmira Yantiyy.anthie79@gmail.comDepartment of Plant Protection, Faculty of Agriculture, Universitas Andalas, Indonesia

Hasmiandy HamidDepartment of Plant Protection, Faculty of Agriculture, Universitas Andalas, Indonesia

R ReflinDepartment of Plant Protection, Faculty of Agriculture, Universitas Andalas, Indonesia

Y YaherwandiDepartment of Plant Protection, Faculty of Agriculture, Universitas Andalas, Indonesia

Dede SuhendraDepartment of Agroecotechnology, Faculty of Agriculture, Universitas Andalas, Indonesia

Doni HariandiDepartment of Agrotechnology, Faculty of Agriculture, Universitas Andalas, Indonesia

Ni Luh SurianiDepartment of Biology, Mathematic and Natural Science Faculty, Udayana University, Indonesia

The tomato (Lycopersicum esculentum Mill.) has substantial scope for development due to its high economic value and large export potential. Ralstonia syzygii subsp. Indonesiensis (RSI) is the cause of bacterial wilt disease which attacks the vascular system in Solanaceae. It can cause large losses in yield and has caused global concern because of its widespread distribution and attack on many important crops. The aim of this research was to identifiy and characterize the ability of indigenous rhizobacterial isolates to control RSI and promote tomato growth. The PGPR traits studied were production of hydrogen cyanide, siderophores, biosurfactant, and ammonia, and protease activity. Bacterial identification was performed using 16S rRNA. Our findings revealed that the strains identified shared some similarities with Bacillus thuringiensis strain ATCC 10792 (IR.2.3.5), B. mycoides strain ATCC 6462 (IR.1.3.4), Bacillus thuringiensis strain IAM 12077 (IR.2.2. 1), Serratia ficaria strain DSM 4569 (IR.3.1.4), Enterobacter oryzendophyticus strain REICA_082 (IR.2.2.7), Cronobacter dublinensis subsp. lausannensis strain E515 (IR.2.2.5) and S. rubidaea strain DSM 4480 (IR.2.2.6). All of the isolates were tested for a variety of abilities related to growth promotion and biocontrol.

Keywords: Ralstonia wilt; 16S rRNA identification; PGPR traits

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