Published date: Jun 07 2022

Journal Title: KnE Life Sciences

Issue title: The First Asian PGPR Indonesian Chapter International e-Conference 2021

Pages: 119–129

DOI: 10.18502/kls.v7i3.11113

Desak Ketut Tristiana SukmadewiAgrotechnology Department, Faculty of Agriculture, Warmadewa University, Indonesia

. SuharjonoDepartment of Biology, Faculty of Mathematics and Natural Science, Brawijaya University, Indonesia

Tirta Kumala DewiResearch Center Biology, Indonesia Institute of Science, Cibinong

M.S. ReddyAuburn University, USA

Sarjiya Antoniussarjiya.antonius@lipi.go.idResearch Center Biology, Indonesia Institute of Science, Cibinong

Clove plants are routinely fertilized with synthetic fertilizer to increase yield. The use of synthetic fertilizer reduces soil productivity. Biofertilizer can be used as an alternative for increasing soil fertility. The goal of this study was to determine the potency of bacteria isolates capable of producing indole acetic acid (IAA) hormone and solubilizing phosphate, and to identify bacteria species from the rhizosphere of clove plants. Soil samples were collected from the clove plants’ rhizosphere, environmental parameters were measured, the potency of IAA-producing and phosphate-solubilizing bacteria was analyzed, and bacteria were molecularly identified. After 48 hours of incubation, isolate TCKI 5 from Karangasem produced the highest IAA hormone levels (19.64 ppm), and isolate TCBP 6 from Buleleng had the highest index of solubilizing phosphate (1.91). A compatibility test between the three best isolates of IAA hormone-producing and phosphate-solubilizing bacteria revealed that TCKI 5 was able to associate with TCBP 6. Isolate TCKI 5 was identified as Leclercia adecarboxylata C107 with a 99.92% similarity, and isolate TCBP 6 as Burkholderia cepacia GJ8 with a 99.61% similarity.

Keywords: Bacteria, clove, Indole Acetic Acid, phosphate, rhizosphere

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