Published date: Nov 28 2019

Journal Title: International Journal of Reproductive BioMedicine

Issue title: International Journal of Reproductive BioMedicine (IJRM): Volume 17, Issue No. 11

Pages: 789–798

DOI: 10.18502/ijrm.v17i10.5486

Mina OmidiDepartment of Physiology, Faculty of Medicine, Student Research Committee, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran

Akram Ahangarpourakramahangarpour@gmail.comPhysiology Research Center, Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Seyed Ali MardPhysiology Research Center, Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Layasadat KhorsandiDepartment of Anatomical Sciences, Faculty of Medicine, Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Background: Aging is accompanied by decreasing general function in the cells and tissues. D-galactose (D-gal) induces aging and plays a role in the pathogenesis of it. Myricitrin is a plant-derived antioxidant.

Objective: The present study was performed to evaluate the effects of myricitrin on antioxidant defense, sex hormone levels, uterus, and ovarian histology in D-galinduced aging female mouse model.

Materials and Methods: In this experimental study, 72 female adult NMRI mice, weighing 30-35 gr, 3-4 months old, were randomly divided into six groups (n = 12/each): (I) Control (vehicle; normal saline), (II) D-gal at 500 mg/kg/d for 45 days, (III-V) D-gal + myricitrin-treated groups (these groups received myricitrin at 5, 10, and 20 mg/kg/d, and (VI) D-gal + 100 mg/kg/d vitamin E orally for the last 28 days. The antioxidant indices were done on the basis of colorimetric method, and sex hormone levels were measured by using enzyme-linked immunosorbent assay kits. Histological assessment of the uterus and ovaries were also evaluated.

Results: D-gal impaired the estrous cycle, also degenerative changes occur in the ovarian follicles and damage to the uterus and ovarian tissue occurrs. In D-gal group, the level of sex hormones (p = 0.03) and the total antioxidant capacity (p = 0.002) decreased, while the level of malondialdehyde and gonadotropins increased (p = 0.03). Myricitrin at lower doses and vitamin E ameliorated the D-gal effects.

Conclusion: These findings suggest that myricitrin can effectively prevent D-galinduced oxidation and aging in mice. The effect of myricitrin was equivalent and sometimes better than vitamin E.

Key words: Aging, D-galactose, Mice, Myricitrin, Vitamin E.

[1] Wang S, He G, Chen M, Zuo T, Xu W, Liu X. The role of antioxidant enzymes in the ovaries. Oxid Med Cell Longev. 2017; 2017: 4371714.

[2] Li YJ, Han Z, Ge L, Zhou CJ, Zhao YF, Wang DH, et al. C-phycocyanin protects against low fertility by inhibiting reactive oxygen species in aging mice. Oncotarget 2016; 7: 17393–17409.

[3] Zhao FF, Zhou YZ, Gao L, Qin XM, Du GH. Advances in the study of the rat model of aging induced by D-galactose. Yao Xue Xue Bao 2017; 52: 347–354.

[4] Ahangarpour A, Najimi SA, Farbood Y. Effects of Vitex agnus-castus fruit on sex hormones and antioxidant indices in a d-galactose-induced aging female mouse model. J Chin Med Assoc 2016; 79: 589–596.

[5] Chen H, Long Y, Guo L. Antiaging Effect of inula britannica on aging mouse model induced by d-galactose. Evid Based Complement Alternat Med 2016; 2016: 1–8.

[6] Chen W, Feng L, Shen Y, Su H, Li Y, Zhuang J, et al. Myricitrin inhibits acrylamide-mediated cytotoxicity in human Caco-2 cells by preventing oxidative stress. Biomed Res Int 2013; 2013: 1–7.

[7] Ahangarpour A, Lamoochi Z, Fathi Moghaddam H, Mansouri SM. Effects of portulaca oleracea ethanolic extract on reproductive system of aging female mice. Int J Reprod BioMed 2016; 14: 205–212.

[8] Huang Q, Gao B, Wang L, Hu YQ, Lu WG, Yang L, et al. Protective effects of myricitrin against osteoporosis via reducing reactive oxygen species and bone-resorbing cytokines. Toxicol Appl Pharmacol 2014; 280: 550–560.

[9] Li J, Zhang M, Ma J. Myricitrin inhibits PDGF-BB-stimulated vascular smooth muscle cell proliferation and migration through suppressing PDGFRβ/Akt/Erk signaling. Int J Clin Exp Med 2015; 8: 21715–21723.

[10] Ahangarpour A, Oroojan AA, Khorsandi L, Kouchak M, Badavi M. Solid lipid nanoparticles of myricitrin have antioxidant and antidiabetic effects on streptozotocinnicotinamide- induced diabetic model and myotube cell of male mouse. Oxid Med Cell Longev 2018; 2018: 7496936.

[11] Kassem ME, Ibrahim LF, Hussein SR, El-Sharawy R, El- Ansari MA, Hassanane MM, et al. Myricitrin and bioactive extract of Albizia amara leaves: DNA protection and modulation of fertility and antioxidant-related genes expression. Pharm Biol 2016; 54: 2404–2409.

[12] Soleimani Mehranjani M, Noorafshan A, Hamta A, Momeni HR, Abnosi MH, Mahmoodi M, et al. Effects of vitamin E on ovarian tissue of rats following treatment with pnonylphenol: A stereological study. Iran J Reprod Med 2010; 8: 1–9.

[13] Ekambaram G, Sampath Kumar SK, Joseph LD. Comparative study on the estimation of estrous cycle in mice by visual and vaginal lavage method. J Clin Diagn Res 2017; 11: AC05–AC07.

[14] Park JH, Choi TS. Polycystic ovary syndrome (PCOS)- like phenotypes in the d-galactose-induced aging mouse model. Biochem Biophys Res Commun 2012; 427: 701–704.

[15] Budni J, Pacheco R, da Silva S, Garcez ML, Mina F, Bellettini-Santos T, et al. Oral administration of d-galactose induces cognitive impairments and oxidative damage in rats. Behav Brain Res 2016; 302: 35–43.

[16] Ahangarpour A, Oroojan AA, Heidari H. Effects of exendin- 4 on male reproductive parameters of D-galactose induced aging mouse model. World J Mens Health 2014; 32: 176–183.

[17] Sekhon LH, Gupta S, Kim Y, Agarwal A. Female infertility and antioxidants. Current Women’s Health Reviews 2010; 6: 84–95.

[18] Agarwal A, Aponte-Mellado A, Premkumar BJ, Shaman A, Gupta S. The effects of oxidative stress on female reproduction: a review. Reprod Biol Endocrinol 2012; 10: 49–80.

[19] Behrman HR, Kodaman PH, Preston SL and Gao S. Oxidative stress and the ovary. J Soc Gynecol Investig 2001; 8 (Suppl.): S40–42.

[20] Kannappan R, Gupta SC, Kim JH, Aggarwal BB. Tocotrienols fight cancer by targeting multiple cell signaling pathways. Genes Nutr 2012; 7: 43–52.

[21] Mohd Mutalip SS, Ab-Rahim S, Rajikin MH. Vitamin E as an antioxidant in female reproductive health. Antioxidants 2018; 7: 1–22.

[22] Bahadori MH, Sharami SH, Fakor F, Milani F, Pourmarzi D, Dalil-Heirati SF. Level of vitamin e in follicular fluid and serum and oocyte morphology and embryo quality in patients undergoing IVF treatment. J Family Reprod Health 2017; 11: 74–81.

[23] Cicek N, Eryilmaz OG, Sarikaya E, Gulerman C, Genc Y. Vitamin E effect on controlled ovarian stimulation of unexplained infertile women. J Assist Reprod Genet 2012; 29: 325–328.

[24] Ahangarpour A, Oroojan AA, Khorsandi L, Kouchak M, Badavi M. Antioxidant effect of myricitrin on hyperglycemia-induced oxidative stress in C2C12 cell. Cell Stress Chaperones 2018; 23: 773–781.

[25] Yan Z, Dai Y, Fu H, Zheng Y, Bao D, Yin Y, et al. Curcumin exerts a protective effect against premature ovarian failure in mice. J Mol Endocrinol 2018; 60: 261–271.

[26] Leeners B, Geary N, Tobler PN, Asarian L. Ovarian hormones and obesity. Hum Reprod Update 2017; 23: 300–321.