Published date: Dec 06 2024

Journal Title: International Journal of Reproductive BioMedicine

Issue title: International Journal of Reproductive BioMedicine (IJRM): Volume 22, Issue No. 10

Pages: 821–836

DOI: 10.18502/ijrm.v22i10.17670

Roghayeh EsfehaniDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Farnaz KhadiviMedical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Jamal ValipourDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Maryam ShabaniDepartment of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Mahya RameshDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Parinaz JavanbakhtDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Davood ZariniDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Sina Mojaverrostamismojaver@sina.tums.ac.irDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Masih HoseiniDepartment of Anatomy, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Background: Testicular ischemia/reperfusion injury, a significant result of testicular torsion, can lead to the risk of male infertility.

Objective: The current study aimed to evaluate the effect of human amniotic membrane-derived mesenchymal stem cells (hAMSCs) secretome on testicular torsion/detorsion (T/D) in mice.

Materials and Methods: All the experiments were performed in the Anatomy Department of Tehran University of Medical Sciences, Tehran, Iran, during the period of March 2023 to December 2023. 40 male NMRI mice (5–7 wk, 25–30 gr) were randomized into: 1) the sham group: mice received sham operations with no other interventions, 2) T/D group, 3) negative control group; torsion detorsion + intratesticular injection of Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12, and 4) the T/D group + hAMSCs secreted factors. Serum testosterone levels, hematoxylin and eosin staining, and sperm quality parameters were used to evaluate the therapeutic effects of hAMSCs secreted factors on the testicular structure and function. Tissue oxidative stress was measured by determining malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase-1. Nuclear factor erythroid 2-related factor 2, Kelch-like ECH-associated protein 1, NAD-dependent deacetylase sirtuin-1, tumor necrosis factor-alpha and tumor protein P53 mRNA expressions were assessed in testis via real-time polymerase chain reaction.

Results: The results showed that hAMSCs secreted factors alleviated testicular T/D injury by attenuating oxidative stress, inflammatory response, and apoptosis via modulating the sirtuin-1/ nuclear factor erythroid 2-related factor 2 / tumor necrosis factor-alpha signaling pathway.

Conclusion: hAMSCs secreted factors increased antioxidative, anti-inflammatory, and antiapoptotic properties which consequently increased testosterone levels, spermatogenesis, and sperm quality parameters.

Keywords: Reperfusion injury, Mesenchymal stem cells, Secretome, Oxidative stress.

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