International Journal of Reproductive BioMedicine
ISSN: 2476-3772
The latest discoveries in all areas of reproduction and reproductive technology.
Established Yazd human foreskin fibroblast lines (#8, #17, and #18) displaying similar characteristics to mesenchymal stromal cells: A lab resources report
Published date: Aug 08 2022
Journal Title: International Journal of Reproductive BioMedicine
Issue title: International Journal of Reproductive BioMedicine (IJRM): Volume 20, Issue No. 7
Pages: 519-528
Authors:
Abstract:
Background: Fibroblasts from different parts of the human body have been used in cell biology, drug discovery and cell therapy studies. One of the most available sources of human fibroblasts is neonatal foreskin. Not only do these cells have woundhealing applications, but they are also the most popular source for pluripotent stem cell biotechnology. Moreover, several studies have indicated that different sources of fibroblasts display similar features to mesenchymal stem cells.
Objective: Generation and establishment of new human foreskin fibroblast cell lines called Yazd human foreskin fibroblasts (YhFFs).
Materials and Methods: In this lab resources study, the production of 3 YhFF cell lines (YhFF#8, YhFF#17, and YhFF#18) is reported. Their biological features were characterized using immunofluorescence, polymerase chain reaction, and flowcytometry for mesenchymal markers such as fibronectin, vimentin, CD44, CD73, CD90, CD105, and hematopoietic markers CD34 and CD45.
Results: The YhFF cell lines were passaged more than 40 times and their normal karyotype was checked using G-binding. Similarly to previous reports, the flow cytometry analysis revealed that the YhFF cell lines displayed mesenchymal stromal cell characteristics.
Conclusion: This study will contribute to the development of clinical-grade cell-based products such as micro-vesicles and exosomes for future therapeutic applications in regenerative medicine.
Key words: Cell therapy, Fibroblasts, Mesenchymal stem/stromal cells, Human embryonic stem cells, Micro-vesicles.
References:
[1] Fang F, Ni K, Cai Y, Ye Z, Shang J, Shen S, et al. Biological characters of human dermal fibroblasts derived from foreskin of male infertile patients. Tissue Cell 2017; 49: 56–63.
[2] Larijani B, Ghahari A, Warnock GL, Aghayan HR, Goodarzi P, Falahzadeh Kh, et al. Human fetal skin fibroblasts: Extremely potent and allogenic candidates for treatment of diabetic wounds. Med Hypotheses 2015; 84: 577–579.
[3] Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, et al. Embryonic stem cell lines derived from human blastocysts. Science 1998; 282: 1145–1147.
[4] Akyash F, Tahajjodi SS, Farashahi Yazd E, Hajizadeh- Tafti F, Sadeghian-Nodoushan F, Golzadeh J, et al. Derivation of new human embryonic stem cell lines (Yazd1-3) and their vitrification using Cryotech and Cryowin tools: A lab resources report. Int J Reprod BioMed 2019; 17: 891–906.
[5] Omidi M, Aflatoonian B, Tahajjodi SS, Khalili MA. Attempts for generation of embryonic stem cells from human embryos following in vitro embryo twinning. Stem Cells Dev 2019; 28: 303–309.
[6] Akyash F, Sadeghian-Nodoushan F, Tahajjodi SS, Nikukar H, Farashahi-Yazd E, Azimzadeh M, et al. Human embryonic stem cells and good manufacturing practice: Report of a 1-day workshop held at Stem Cell Biology Research Center, Yazd, 27th April 2017. Int J Reprod BioMed 2017; 15: 255–256.
[7] Ma Y, Gu J, Li C, Wei X, Tang F, Shi G, et al. Human foreskin fibroblast produces interleukin-6 to support derivation and self-renewal of mouse embryonic stem cells. Stem Cell Res Ther 2012; 3: 29.
[8] Kim KM, Heo DR, Lee JY, Seo CS, Chung SK. High-efficiency generation of induced pluripotent stem cells from human foreskin fibroblast cells using the Sagunja-tang herbal formula. BMC Complement Altern Med 2017; 17: 529.
[9] Alt E, Yan Y, Gehmert S, Song YH, Altman A, Gehmert S, et al. Fibroblasts share mesenchymal phenotypes with stem cells, but lack their differentiation and colony-forming potential. Biol Cell 2011; 103: 197–208.
[10] Yin K, Wang S, Zhao RC. Exosomes from mesenchymal stem/stromal cells: A new therapeutic paradigm. Biomark Res 2019; 7: 8.
[11] Maqsood M, Kang M, Wu X, Chen J, Teng L, Qiu L. Adult mesenchymal stem cells and their exosomes: Sources, characteristics, and application in regenerative medicine. Life Sci 2020; 256: 118002.
[12] Cano A, Perez-Moreno MA, Rodrigo I, Locascio A, Blanco MJ, del Barrio MG, et al. The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression. Nat Cell Biol 2000; 2: 76–83.
[13] Akyash F, Javidpou M, Farashahi-Yazd E, Golzadeh J, Hajizadeh-Tafti F, Aflatoonian R, et al. Characteristics of the human endometrial regeneration cells as a potential source for future stem cell-based therapies: A lab resources study. Int J Reprod BioMed 2020; 18: 943–950.
[14] Javidpou M, Seifati SM, Farashahi-Yazd E, Hajizadeh- Tafti F, Golzadeh J, Akyash F, et al. Mesenchymal stem/stromal-like cells from diploid and triploid human embryonic stem cells display different gene expression profiles. Iran Biomed J 2021; 25: 99–105.
[15] Mendez MG, Kojima SI, Goldman RD. Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition. FASEB J 2010; 24: 1838–1851.
[16] Ugurlu B, Karaoz E. Comparison of similar cells: Mesenchymal stromal cells and fibroblasts. Acta Histochem 2020; 122: 151634.
[17] Sadeghian-Nodoushan F, Aflatoonian R, Borzouie Z, Akyash F, Fesahat F, Soleimani M, et al. Pluripotency and differentiation of cells from human testicular sperm extraction: An investigation of cell stemness. Mol Reprod Dev 2016; 83: 312–323.
