ESPOCH Congresses: The Ecuadorian Journal of S.T.E.A.M.
ISSN: 2789-5009
Leading Ecuadorian research in science, technology, engineering, arts, and mathematics.
Obtention of Antimicrobial Fibers Type Core/Shell Pla/Pvoh-Lae By Coaxial Electrospinning
Published date: Aug 26 2021
Journal Title: ESPOCH Congresses: The Ecuadorian Journal of S.T.E.A.M.
Issue title: Volume 1, Issue 1
Pages: 283–293
Authors:
Abstract:
Coaxial electrospinning (EC) is a technology that allows the encapsulation of active compounds, such as ethyl lauroyl arginate (LAE), in shell/core structures, in order to develop new antimicrobial materials for food packaging that slow down the release of active compounds and extend the food’s shelf life. For this reason, the objective of this study was to develop antimicrobial fibers shell/core type by EC. Two polymers with different hydrophilic character, polylactic acid (PLA) for the shell and polyvinyl alcohol (PVOH) and LAE for the core, were used to obtain PLA/PVOH-LAE fibers and slow the release of the antimicrobial compound. The morphology of fibers was evaluated by optical microscopy and their thermal properties through thermogravimetric analyses (TGA). LAE release studies were carried out in a fatty food simulant (ETOH 95%), and was compared with the minimum inhibitory concentration (MIC) values of LAE against a gram-positive bacteria, Listeria innnocua. The optical micrographs showed the obtaining of the shell/core structure with an average diameter of approximately 0.6 µm, and the TGA analyses demonstrated the thermal protection of LAE by the shell of the fibers. Released LAE reached the equilibrium state in ETOH 95% during the first 3 hours, maintaining a higher concentration than the MIC value obtained in L. innnocua (10 ppm). The results demonstrate that new packaging materials with antimicrobial activity such as PLA/PVOH-LAE polymeric fibers with a shell/core structure can be obtained through the coaxial electrospinning technique.
Keywords: coaxial electrospinning, ethyl lauroyl arginate, fibers, shell/core, food packaging.
Resumen
El electrohilado coaxial (EC) es una tecnología que permite encapsular compuestos activos como el etil lauroil arginato (LAE) en estructuras tipo pared/núcleo con la finalidad de desarrollar nuevos materiales antimicrobianos para el envasado de alimentos que ralenticen la liberación del compuesto y extiendan la vida útil del alimento. Por tal razón, el objetivo de este estudio consistió en desarrollar fibras antimicrobianas tipo pared/núcleo mediante EC. Dos polímeros con distinto carácter hidrofílico como el poli (ácido láctico) (PLA) para la pared y poli (alcohol vinílico) (PVOH) y LAE para el núcleo se utilizaron para obtener las fibras PLA/PVOH-LAE, y ralentizar la liberación del compuesto antimicrobiano. La morfología de las fibras se evaluó mediante microscopía óptica y sus propiedades térmicas mediante análisis termogravimétricos (TGA). Se realizaron estudios de liberación del LAE en un simulante de alimentos graso (ETOH 95%), y se comparó con la concentración mínima inhibitoria (MIC) hacia una bacteria Gram positiva Listeria innnocua. Las micrografías ópticas evidenciaron la obtención de la estructura pared/núcleo con un diámetro promedio de 0,6 µm aproximadamente, y el análisis TGA demostró la protección térmica del LAE por la pared de las fibras. El LAE liberado alcanzó el estado de equilibrio en ETOH 95% durante las 3 hr iniciales, manteniendo una concentración superior a la MIC obtenida para L. innnocua (10 ppm). Los resultados demuestran que nuevos materiales de envase con actividad antimicrobiana como fibras poliméricas PLA/PVOH-LAE con estructura pared/núcleo pueden ser obtenidos mediante la técnica de electrohilado coaxial.
Palabras clave: electrohilado coaxial, etil lauroil arginato, fibras, pared/núcleo, envase de alimentos.
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