Published date: Aug 29 2021

Journal Title: ESPOCH Congresses: The Ecuadorian Journal of S.T.E.A.M.

Issue title: Volume 1, Issue 3

Pages: 1072–1080

DOI: 10.18502/espoch.v1i3.9533

Anabell Del Rocío Urbina-Salazargata-any@hotmail.comUniversidad Técnica de Manabí, Instituto de Ciencias Básicas, Portoviejo, Ecuador

Alberto Renato Inca-TorresUniversidad Técnica de Manabí, Instituto de Ciencias Básicas, Portoviejo, Ecuador

Bryan Anthony Urbina-SalazarUniversidad Técnica de Ambato, Facultad de Ciencias de la Salud, Carrera de Medicina, Ambato, Ecuador

Valeria Fernanda Inca-TorresHospital Básico Baños, Baños, Ecuador

Juan BautistaUniversidad de Sevilla, Facultad de Farmacia, Departamento de Bioquímica y Biología Molecular, Sevilla, España

Ergotioneine (ERG) has potential applications as food additive and as an antioxidant for the treatment and/or prevention of diseases related to oxidative stress. In this work, we study the nature of antioxidant activity and investigate the effect of aqueous extracts of Agaricus bisporus enriched with ergotioneine (EAHE-ERG), a natural product, obtained through enzymatic digestion and membrane technology, in cellular response induced by UV, in crops fibroblasts. EAHE-ERG suppressed by the increase of TNF-α by UV-B radiation. Furthermore, in fibroblasts exposed to UV-A, EAHE-ERG suppressed the expression of matrix metalloproteinase-1 protein (MMP-1) in almost 50% and reduced the RNAm expression from MMP-1. From these results, we conclude that EAHE-ERG can reduce anti-aging effects of the skin from UV radiation by removing O₂ and 1O₂, and the reduction protease signs and inflammatory activity.

Keywords: Ergotioneine, Agaricus bisporus, Antioxidant, Nutraceutical, Anti-inflammatory.

Resumen

La ergotioneina (ERG) tiene aplicaciones potenciales como un aditivo alimentario y como un antioxidante para el tratamiento y/o prevención de enfermedades relacionadas con el estrés oxidativo. En este trabajo estudiamos la naturaleza de la actividad antioxidante e investigamos el efecto de los extractos acuosos de Agaricus bisporus enriquecidos con ergotioneina (EAHEERG), un producto natural, obtenido mediante digestión enzimática y tecnología de membrana, en respuesta celular inducida por UV, en fibroblastos cultivados. EAHE-ERG suprimió la regulación por incremento de TNF-α mediante radiación UV-B. Además, en fibroblastos expuestos a UV-A, EAHE-ERG suprimió la expresión de proteína de metaloproteinasa-1 de matriz (MMP-1) en casi 50% y redujo la expresión de ARNm de MMP-1. A partir de estos resultados, concluimos que EAHE-ERG puede reducir los efectos antienvejecimiento de la piel después de la irradiación UV mediante la eliminación de O₂ y 1O₂, y la reducción de las señales de proteasa y actividad inflamatoria.

Palabras Clave: Ergotioneina, Agaricus bisporus, Antioxidante, Nutracéutico, Antiinflamatorio.

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