Sudan Journal of Medical Sciences

ISSN: 1858-5051

High-impact research on the latest developments in medicine and healthcare across MENA and Africa

Molecular diagnosis of Shigella, Salmonella and Campylobacter by multiplex Real-time PCR in stool culture samples in Ouagadougou (Burkina Faso)

Published date: Sep 06 2017

Journal Title: Sudan Journal of Medical Sciences

Issue title: Sudan JMS: Volume 12 (2017), Issue No. 3

Pages: 163-173

DOI: 10.18502/sjms.v12i3.931

Authors:

Salfo SawadogoEmail: Saw_salfo@yahoo.fr
Affiliation: Pietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina Faso
Biography:

Birama DiarraEmail: diarra.birama679@gmail.com
Affiliation: Pietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina Faso
Biography:

Cyrille BIsseyeEmail: cbisseye@gmail.com
Affiliation: University of Science and Technique of Masuku
Biography: Department of Biology, Assistant Professor

Tegwindé Rebeca CompaoreEmail: rebecca23fr@yahoo.fr
Affiliation: Pietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina Faso
Biography:

Florencia W. DjigmaEmail: flodjig@yahoo.fr
Affiliation: Pietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina Faso
Biography:

Djénéba OuermiEmail: oddianemed@gmail.com
Affiliation: Pietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina Faso
Biography:

Aboubakar S. OuattaraEmail: ouattabs@univ-ouaga.bf
Affiliation: CRSBAN, University of Ouaga I Pr Joseph KI-ZERBO, 03 BP 7131 Ouagadougou 03, Burkina Faso
Biography:

Jacques SimporeEmail: jacques.simpore@yahoo.fr
Affiliation: Pietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina Faso
Biography:

Salfo Sawadogo - Saw_salfo@yahoo.fr

Birama Diarra - diarra.birama679@gmail.com

Cyrille BIsseye - cbisseye@gmail.com

Tegwindé Rebeca Compaore - rebecca23fr@yahoo.fr

Florencia W. Djigma - flodjig@yahoo.fr

Djénéba Ouermi - oddianemed@gmail.com

Aboubakar S. Ouattara - ouattabs@univ-ouaga.bf

Jacques Simpore - jacques.simpore@yahoo.fr

Abstract:

ABSTRACT:

Background: Bacteriological diagnosis of Campylobacter spp, Salmonella spp and Shigella spp could be necessary in the case of infectious gastroenteritis syndrome.

The objective of this study was to diagnose concomitantly the three enteropathogenic bacteria by multiplex Real-Time PCR in stool culture samples in Ouagadougou (Burkina Faso).

Materials and Methods: The study was conducted from February 5th to March 9th, 2013. Two hundred stool samples were received during the study period. The bacteria were identified by bacterial culture following by multiplex Real-Time PCR.

Results: Shigella spp and Campylobacter spp were sought by culture in all 200 samples. Enteropathogenic E. coli was sought only in 37 samples from all children under 2 years old. The bacterial culture was positive in 12 stool samples. Shigella spp and Salmonella spp. were isolated respectively in 5 (2.5%) and 3 samples (1.5%). Enteropathogenic E. coli was isolated in 10.8% (4/37) of the samples tested.

The multiplex real-time PCR identified bacteria in 20 patients, including 17 cases of Shigella spp., 1 case of Salmonella spp. and 2 cases of Campylobacter spp.

Conclusions: This study has highlighted the low frequency of 3 sought bacterial genera in stool samples. It has also demonstrated a significant difference between the culture and the multiplex Real-Time PCR method in the diagnosis of Shigella.

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