Sudan Journal of Medical Sciences

ISSN: 1858-5051

High-impact research on the latest developments in medicine and healthcare across MENA and Africa

Isolation, Extraction, and Characterization of Verotoxin-producing Escherichia coli O157:H7 from Diarrheal Stool Samples

Published date: Mar 31 2022

Journal Title: Sudan Journal of Medical Sciences

Issue title: Sudan JMS: Volume 17 (2022), Issue No. 1

Pages: 116-127

DOI: 10.18502/sjms.v17i1.10689

Authors:

Muhammad Musthafa PoyilEmail: N/A
Affiliation: Department of Basic Medical Sciences, College of Medicine, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia
Biography:

Ponmurugan KaruppiahEmail: pmusthu@gmail.com
Affiliation: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia
Biography:

Suresh S. S. RajaEmail: N/A
Affiliation: 4Department of Microbiology, Government Arts and Science College, Perambalur, Tamil Nadu, India
Biography:

P. SasikumarEmail: N/A
Affiliation: Department of Microbiology, The Madura College, Madurai, Tamil Nadu, India
Biography:

Muhammad Musthafa Poyil

Ponmurugan Karuppiah - pmusthu@gmail.com

Suresh S. S. Raja

P. Sasikumar

Abstract:

Background: Escherichia coli O157:H7 infection causes hemorrhagic colitis and is diagnosed based on symptoms such as cramps, stomach pain, and watery diarrhea. Shiga-like toxins (Verotoxin) produced by Escherichia coli O157:H7 damages endothelial cells of both kidney and brain, causing renal dysfunction and neurological problems.

Methods: The present study focuses on identifying the prevalence of Verotoxin-producing Escherichia coli O157:H7 among diarrheal inpatients at Erode Government Hospital, India, and its antibiogram. Further, the Verotoxins were characterized by using SDS-PAGE analysis. A total of 123 samples were collected both from diarrheal stools, and strains from 37 samples (43.02 %) were found to have the presence of E. coli.  The organisms were identified based on their colony morphology on various media, cell morphology, and biochemical tests. The Shiga-like toxin production was identified by non-fermentation of sorbitol on SMAC agar plates. Confirmation of Shiga-like toxin was performed using agglutination assay.

 Results: In total, 12 isolates showed agglutination and these isolates were confirmed to be E. coli O157:H7. The molecular weight of the Verotoxin was found to be between 20 and 29 kD. The antibiogram profile of the four isolated strains against 10 standard antibiotics was determined.

Conclusion: The results of this study show the occurrence of drug resistance on hemorrhagic colitis causing E. coli O157:H7.

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