Sudan Journal of Medical Sciences

ISSN: 1858-5051

High-impact research on the latest developments in medicine and healthcare across MENA and Africa

Isolation, Extraction, and Characterization of Verotoxin-producing Escherichia coli O157:H7 from Diarrheal Stool Samples

Published date: Mar 31 2022

Journal Title: Sudan Journal of Medical Sciences

Issue title: Sudan JMS: Volume 17 (2022), Issue No. 1

Pages: 116-127

DOI: 10.18502/sjms.v17i1.10689

Authors:

Muhammad Musthafa PoyilDepartment of Basic Medical Sciences, College of Medicine, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia

Ponmurugan Karuppiahpmusthu@gmail.comDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

Suresh S. S. Raja4Department of Microbiology, Government Arts and Science College, Perambalur, Tamil Nadu, India

P. SasikumarDepartment of Microbiology, The Madura College, Madurai, Tamil Nadu, India

Abstract:

Background: Escherichia coli O157:H7 infection causes hemorrhagic colitis and is diagnosed based on symptoms such as cramps, stomach pain, and watery diarrhea. Shiga-like toxins (Verotoxin) produced by Escherichia coli O157:H7 damages endothelial cells of both kidney and brain, causing renal dysfunction and neurological problems.

Methods: The present study focuses on identifying the prevalence of Verotoxin-producing Escherichia coli O157:H7 among diarrheal inpatients at Erode Government Hospital, India, and its antibiogram. Further, the Verotoxins were characterized by using SDS-PAGE analysis. A total of 123 samples were collected both from diarrheal stools, and strains from 37 samples (43.02 %) were found to have the presence of E. coli.  The organisms were identified based on their colony morphology on various media, cell morphology, and biochemical tests. The Shiga-like toxin production was identified by non-fermentation of sorbitol on SMAC agar plates. Confirmation of Shiga-like toxin was performed using agglutination assay.

 Results: In total, 12 isolates showed agglutination and these isolates were confirmed to be E. coli O157:H7. The molecular weight of the Verotoxin was found to be between 20 and 29 kD. The antibiogram profile of the four isolated strains against 10 standard antibiotics was determined.

Conclusion: The results of this study show the occurrence of drug resistance on hemorrhagic colitis causing E. coli O157:H7.

Keywords: E. coli, Verotoxin, diarrhea, hemorrhagic colitis

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