Published date: Sep 11 2017

Journal Title: KnE Life Sciences

Issue title: International Conference on Natural Resources and Life Sciences (NRLS-2016)

Pages: 131-138

DOI: 10.18502/kls.v3i5.985

Liling Delila

Evi Susantievi.susanti.fmipa@um.ac.idDept. of Chemistry, Faculty of Science and Mathematics, Universitas Negeri Malang, Jalan Semarang No. 5, 65145, Malang, Indonesia

Eli Hendrik Sanjaya

The aim of this research is to obtain indigenous fungus producing lignin peroxidase from the Cocoa Plantation in Sepawon Kediri Regency, Indonesia. Isolation was done using Minimum Salt Medium alkali lignin (MSM - L), purification was performed using monospore technique, lignin peroxidase activity was screened by agar medium containing methylene blue, and microscopic morphology of fungi was studied using slide culture technique. Five isolates of fungus were obtained and three isolates of fungus with different morphology were purified. The screening results showed that only two fungus isolates were indicated as the indigenous fungus producing lignin peroxidase were KLUM₁ and KLUM₂. KLUM₁ was morphologycally predicted as Sporotricum aereum and KLUM₂ was predicted close to genus Crysosporium sp.

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