Published date: Jul 11 2017

Journal Title: KnE Life Sciences

Issue title: The 4th International Conference on Biological Science (2015)

Pages: 125-135

DOI: 10.18502/kls.v3i4.696

Kusmiati .kusmiati02@yahoo.com

Ni Wayan Sri Agustini

Broccoli (Brassica oleracea L. var. italica) is one vegetable that is often consumed by people with high nutrient content i.e protein, fat, vitamin C, fiber, potassium and calcium. Carotenoids and glutathione are contained in broccoli that are antioxidants. Lutein is a carotenoid group contained in broccoli is capable of reducing the accumulated products of free radicals in the body. The purpose of this research is to extract and identify compounds lutein from flowers of broccoli and test its potential as antiradical to the compound ABTS (2,2-Azinobis acid, 3-ethyl benzatiazolin-6-Sulfonat). Identification of lutein compounds qualitatively by TLC showed Rf value of 0.72 cm. Analysis of Lutein by HPLC showed a peak at the retention time of 3:03 min, approaching retention time of lutein standard 3:01 min. Test of potency of antiradical ABTS showed that lutein extract from flower of broccoli has antioxidant activity with IC₅₀ value of 157.527 8 µg · mL^–1.  Fractionation of lutein extract using column chromatography with Silica gel 60 as stationary phase and mixture of methanol : chloroform : n-hexane (4:20:8) as mobile phase resulted in 4 fractions with antioxidant activity increased. Fraction 3 has the highest antiradical ABTS activity with IC₅₀ 39.584 2 µg · mL^–1. Vitamin E is used as a standard had antiradical ABTS activity IC₅₀ 5.303 6 µg · mL^–1.

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