Published date: Mar 25 2019

Journal Title: KnE Life Sciences

Issue title: The 1st International Conference on Health, Technology and Life Sciences (ICO-HELICS)

Pages: 114–122

DOI: 10.18502/kls.v4i12.4164

Muthmainah .muthmainah.fkuns@staff.uns.ac.id

M T Giani

N Wiyono

R H Setyaningrum

R D Yudhani

Chronic stress is associated with the development of depression. It can trigger structural and neurobehavioral changes in the brain and has been shown to induce depressivelike behavior in animals. An enriched environment can modulate the structure and function of the brain by altering the expression of various genes and proteins as well as affecting neurotransmitters’ activity. The hippocampus plays an important role in
controlling the networks for mood regulation and has been implicated in the course of depression. This study aimed to investigate the effect of an enriched environment on the depressive-like behavior and hippocampal structure in rats after unpredictable chronic mild stress (UCMS) exposure. Male Wistar rats (Rattus novergicus) were divided into three groups, each consisting of 6 rats including the control, UCMS and UCMS+EE
group. Unpredictable chronic mild stress and EE were given for 21 days. Body weight gain, depressive-like behavior, and hippocampal structure were evaluated at the end of the experiment. Depressive-like behavior was assessed with Forced Swim Test (FST) and Sucrose Preference Test (SPT). Thickness of the pyramidal layer of CA1 and CA3 area were measured with histologic examination to see changes in the hippocampal
structure. Data were analyzed using One-Way ANOVA or Kruskal-Wallis followed by multiple comparison post hoc test. The enriched environment could significantly maintain body weight gain (p = 0.036) and rat’s preference to sucrose solution (p =0.001) in a stressful condition. Enriched environment reduced immobility time in FST but it was not statistically significant (p = 0.177). There was a significant difference in the thickness of CA1 and CA3 pyramidal layer of the hippocampus among groups (p=0.015 and p=0.019 respectively). Stress markedly decreased the thickness of CA1 and CA3 pyramidal layer (p=0.014 and 0.011 respectively). The enriched environment can ameliorate stress-induced depressive-like behavior and alteration in hippocampal
structure in rats.

Keywords: Environmental enrichment, depression, stress, hippocampus

[1] Muthmainah and Nurwati I 2016 Med Acupunct 28(6) 301-07


[2] Hay E L and Diehl M 2010 Psychol Aging 25(1) 118-31


[3] Gold P W, Machado-Vieira R and Pavlatou M G 2015 Neural Plast 581976


[4] Armario A, Escorihuela R M and Nadal R 2008 Neurosci Biobehav Rev 32(6) 1121-1135


[5] Nollet M, Guisquet al and Belzung C 2013 Curr Protoc Pharmacol 5.65.1-5.65.17


[6] World Health Organization. Depression facts sheet [homepage on the internet]. No date [cited 2016 May 25]. Available from: http://www.who.int/mediacentre/ factsheets/fs369/en/.


[7] Li Y, Mu Y and Gage F H 2009 Curr Top Dev Biol 87 149-74


[8] Weinberger D R 1999 Biol Psychiatry 45(4) 395-02


[9] Willner P 2005 Neuropsychobiology 52(2) 90-110


[10] Anacker C 2014 Curr Top Behav Neurosci. 18 25-43


[11] Watanabe Y, Gould E, Daniels D C, Cameron H and McEwen B S 1992 Eur J Pharmacol. 222(1) 157-62


[12] Woolley C S, Gould E and McEwen B S 1990 Brain Res. 531(1-2) 225-31


[13] Sheline Y I, Wang P W, Gado M H, Csernansky J G and Vannier M W 1996 Proc Natl Acad Sci U S A 93(9) 3908-13.


[14] Apostolo J, Bobrowicz-campos E, Rodrigues M, Castro In and Cardoso 2016 Int J Nurs Stud 58 59-70


[15] Goldberg H I, Wagner E H, Fihn S D, Martin D P, Horowitz C R, Christensen D B, Cheadle A D, Diehr P and Simon G 1998 Jt Comm J Qual Improv 24(3) 130-42


[16] Fava M 2003 Biol Psychiatry 53(8) 649-59


[17] Arroll B, Macgillivray S, Ogston S, Reid I, Sullivan F, Williams B and Crombie I 2005 Ann Fam Med. 3(5) 449-56


[18] Pigott H E, Leventhal A M, Alter G S and Boren J J 2010 Psychother Psychosom 79267–79


[19] Mileva G R and Bielajew C 2015 Behav Brain Res 293 208-16


[20] Hutchinson K M, McLaughlin K J, Wright R L, Ortiz J B, Anouti D P, Mika A, Diamond D M and Conrad C D 2012 Neurobiol Learn Mem. 97 250-60


[21] Chabry J, Nicholas S, Cazareth J, Murris E, Guyon A, Glaichenhaus N, Heurteaux C and Petit-Paitel A 2015 Brain Behav Immun. 50 275-87


[22] Kotloski RJ and Sutula TP 2015 Exp Neurol. 264 121-6


[23] Marmol F, Rodriguez C A, Sanchez J and Chamizo V D 2015 Brain Res. 1613 120-9


[24] Konkle A T, Kentner A C, Baker L S, Stewart A and Bielajew C 2010 J Am Assoc Lab Anim Sci 49(4) 427-36


[25] Simpson J and Kelly J P 2011 Behav Brain Res 222 246-64


[26] Yankelevitch-Yahav R, Franko M, Huly A and Doron R 2015 J Vis Exp. 97 52587


[27] Veena J, Srikumar B N, Raju T R and Shankaranarayana R B S 2009 Neurosci Lett. 55(3) 178-82


[28] Kotloski R J and Sutula T P 2014 Exp Neurol 264 121-6


[29] Zeeni N, Bassil M, Fromentin G, Chaumontet C, Darcel N, Tome D and Daher C F 2015 Physiol Behav 139 41-49


[30] Jeong J Y, Lee D H and Kang SS 2013 Endocrinol Metab 28(4) 288–96


[31] Garret J E and Wellman C L 2009 Neuroscience 162(1) 195-207


[32] Venero C, Tilling T, Hermans-Borgmeyer I, Schmidt R, Schachner M and Sandi C Neuroscience 115(4) 1211-9


[33] Sari D C R, Aswin S, Susilowati R, Ar-Rochmah M, Prakosa D, Romi M, Tranggono U and Arfian N 2014 J. Psych. 1(1) 61-66