Published date: Feb 28 2019

Journal Title: KnE Life Sciences

Issue title: The 3rd International Meeting of Public Health and the 1st Young Scholar Symposium on Public Health

Pages: 235–242

DOI: 10.18502/kls.v4i10.3725

F Kurniasari

T Dian Susanti

I Mulyana

H Atmajaya

D Hikmat Ramdhandoni@ui.ac.id

Diesel engine exhaust is categorized as carcinogenic to human (group 1) by International Agency for Research on Cancer (IARC) in 2012. Transportation is the biggest contributor of diesel exhaust pollutant. Polycyclic Aromatic Hydrocarbons (PAHs) are the major compound of diesel exhaust that can be found on gas and particle phases. 1-Hydroxypyrene (1-OHP), a metabolite of pyrene, has been used extensively as a biological monitoring of exposure to PAHs. This study aimed to analyze the concentration of urinary 1-OHP related to diesel engine exhaust among vehicle testing mechanics at Vehicle Testing Center Cilincing, North Jakarta. Non-occupational factors such as exposure to cigarette smoke, consumption to grilled food, exposure to open
fire, and the presence of pollutant near respondent’s residence are also analyzed. Diesel exhaust exposure was measured by its particle phase, which is personal concentration of PM2.5, PM1, PM0.5, and PM0.25. The subject was 19 vehicle testing mechanics and 18 non-mechanics as the control group. Personal concentration of particulate matter collected using Leland Legacy pump and Sioutas Cascade Impactor and analyzed using gravimetric method. Urinary 1-OHP analyzed using High-Performance Liquid Chromatography with fluorescence detector. Personal concentration of PM2.5, PM1,PM0.5, and PM0.25 are 232.23 µg/m3, 190.58 µg/m3, 164.73 µg/m3, and 140.10 µg/m3. Concentration of urinary 1-OHP ranging from 11.72 to 61.88 µg/or creatinine. The result showed that mean concentration of 1-Hydroxypyrene of mechanic group is significantly higher than non-mechanic group (p=0.001). There is a positive correlation between particulate matter concentration in all size and urinary 1-OHP concentration. There’s no significant correlation between non-occupational factors and urinary 1-OHP concentration. Particulate matter related to diesel engine exhaust is the main contributor of the increase of urinary 1-OHP concentration among vehicle testing mechanics.

Keywords: 1-Hydroxypyrene, diesel exhaust, particulate matter, vehicle testing

[1] BPS. Perkembangan Jumlah Kendaraan Bermotor Menurut Jenis Tahun 1987-2013 [Internet]. 2014. Available from: http://www.bps.go.id/linkTabelStatis/view/id/1413


[2] IARC. IARC: DIESEL ENGINE EXHAUST CARCINOGENIC Lyon,. 2012; Available from: https://www.iarc. fr/en/media-centre/pr/2012/pdfs/pr213_E.pdf


[3] OSHA. Health Alert: Diesel Exhaust/Diesel Particulate Matter. 2013;1–4. Available from: https://www. osha.gov/dts/hazardalerts/diesel_exhaust_hazard_alert.html


[4] US EPA. Health Assessment Document For Diesel Engine Exhaust. Washington, DC: U.S. Environmental Protection Agency; 2002.


[5] Birch ME. Monitoring of Diesel Particulate Exhaust in the Workplace. NIOSH Manual Anal Methods. 2003;229–59.


[6] WHO. Polycyclic Aromatic Hydrocarbons (PAHs) Factsheet. 2000; Available from: http://www.euro.who.
int/__data/assets/pdf_file/0015/123063/AQG2ndEd_5_9PAH.pdf


[7] Ifegwu C, Osunjaye K, Fashogbon F, Oke K, Adeniyi A, Anyakora C, et al. Urinary 1-Hydroxypyrene as a Biomarker to Carcinogenic Polycyclic Aromatic Hydrocarbon Exposure. Biomark Cancer [Internet]. 2012;4:7. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid= 3791913&tool=pmcentrez&rendertype=abstract


[8] Adonis M, MartıV. Susceptibility and Exposure Biomarkers in People Exposed to Pahs from Diesel Exhaust. 2003;144:3–15.


[9] Huang W, Smith TJ, Ngo L, Wang T, Chen H, Wu F, et al. Characterizing and Biological Monitoring of Polycyclic Aromatic Hydrocarbons in Exposures to Diesel Exhaust Characterizing and Biological Monitoring of Polycyclic Aromatic Hydrocarbons in Exposures to Diesel Exhaust. 2007;41(8):2711–6.


[10] Kuusimäki L, Peltonen Y, Mutanen P, Peltonen K, Savela K. Urinary Hydroxy-Metabolites of Naphthalene, Phenanthrene and Pyrene As Markers of Exposure to Diesel Exhaust. Int Arch Occup Environ Health. 2004;77(1):23–30.


[11] Scheepers PTJ, Anzion R, Micka V, Poole J, Coggon D, Bos RP. Evaluation of the Use of 1-Nitropyrene, Pyrene, and 1-Hydroxypyrene As Markers of Diesel Exhaust Exposure in Underground Mining: Results From the Biomodem Study. Polycycl Aromat Compd [Internet]. 2004;24(4–5):405–17. Available from: http://www.tandfonline.com/doi/abs/10.1080/10406630490468685


[12] Rizky ZP, Yolla PB, Ramdhan DH. Particulate matter 2.5 (PM2.5) Personal Exposure Evaluation on Mechanics and Administrative Officers at the Motor Vehicle Testing Center at Pulo Gadung, DKI Jakarta. Rev Environ Health. 2016;31(1):21–7.


[13] Jongeneelen F., Anzion RBM, Henderson PT. Determination of Hydroxylated Metabolites of Polycyclic Aromatic Hydrocarbons in Urine. 1987;413:227–32.


[14] Warouw SP. Studi Analisis Risiko Kesehatan Pemajanan Carcinogen Benzo(a)pyrene Pada Petugas Pintu Tol di Jabotabek. 2007.


[15] Wispriyono B, Sari M, Hartono B, Kusnoputranto H. Concentration of Urinary 1-Hydroxypyrene (1-OHP) in Bus Drivers as a Biomarker of Polycyclic Aromatic Hydrocarbons (PAHS) Exposure in Depok City , Indonesia. 2015;3(6):784–9.


[16] Sánchez-Coello OR, Ynoue RY, Martins LD, Astolfo R, Miranda RM, Freitas ED, et al. Vehicular Particulate Matter Emissions in Road Tunnels in Sao Paulo, Brazil. 2009;241–9.


[17] Campo L, Addario L, Buratti M, Scibetta L, Longhi O, Valla C, et al. Biological monitoring of exposure to polycyclic aromatic hydrocarbons by determination of unmetabolized compounds in urine. Toxicol Lett. 2006;162(2–3 SPEC. ISS.):132–8.


[18] Seidel A, Dahmann D, Krekeler H, Jacob J. Biomonitoring of Polycyclic Aromatic Compounds in The Urine of Mining Workers Occupationally Exposed to Diesel Exhaust. Int J Hyg Environ Health [Internet]. 2002;204(5–6):333–8. Available from: http://www.ncbi.nlm.nih.gov/pubmed/11885357


[19] Buratti M, Campo L, Fustinoni S, Cirla PE, Martinotti I, Cavallo D, et al. Urinary Hydroxylated Metabolites Of Polycyclic Aromatic Hydrocarbons as Biomarkers of Exposure in Asphalt Workers. Biomarkers. 2007;12(3):221–39.


[20] Yuan TH, Shie RH, Chin YY, Chan CC. Assessment of the Levels of Urinary 1-Hydroxypyrene and Air Polycyclic Aromatic Hydrocarbon in PM2.5 for Adult Exposure to The Petrochemical Complex Emissions. Environ Res [Internet]. 2015;136:219–26. Available from: http://dx.doi.org/10.1016/j.envres.2014.10.007