KnE Life Sciences
ISSN: 2413-0877
The latest conference proceedings on life sciences, medicine and pharmacology.
The Effect of Blue Green Algae (Spirulina platensis) Extract in White Rat (RattusNorvegicus) Treated with Excessive Physical Exercise on Leydig Cell Number and Seminiferous Tubules Diameter
Published date: Dec 03 2017
Journal Title: KnE Life Sciences
Issue title: The Veterinary Medicine International Conference (VMIC)
Pages: 684-693
Authors:
Abstract:
The aim of this study was to investigate the effect of Spirulina platensis extract on the amount of Leydig cells, amount of necrotic Leydig cells, and diameter of seminiferous tubules of rats treated with excessive physical exercise. The excessive physical exercise was done through 60 minutes of swimming for 35 days. Twenty white male rats were randomly divided into five groups i.e C-, control group, C+, a group was only receive swimming, T1, T2, and T3 was received 300, 600, and 1200 mg/kg BW of Spirulina platensis and swimming. Spirulina platensis extract was given orally once a day before swimming. ANOVA followed by Duncan analysis showed significant differences among treatments. Excessive physical exercise influenced on male reproduction system through decreasing the amount of Leydig cells. The conclusion was a dose of 1200 mg/kg BW of Spirulina platensis extract could maintain the amount of Leydig cells, protected the Leydig cells from becoming necrotic, and increased the diameter of seminiferous tubules.
Keywords: Rattus Norvegicus, Spirulinaplatensis, excessive physical exercise, Leydig cells, seminiferous tubules diameter
References:
1] Manna, I., K Janna and P K Samantha. 2003. Effect of Different Intesities of Swimming Exercise on Testicular Oxidative Stress and Reproductive Dysfunction in Mature Male Albino Wistar Rats. Indian Journal of Experimental Biology. 42: 816-822
[2] Haskell, William L., I-Min Lee, Russell R. Pate, Kenneth E., Powell, Steven N. Blair, Barry A. Franklin, Caroline A. Macera, Gregory W. Heath, Paul D. Thompson, Adrian Bauman. 2007. Physical Activity and Public Health Updated Recommendation for Adults. American College of Sports Medicine and the American Heart Association. Available from: http://circ.ahajournals.org. Accessed: May, 21th 2015.
[3] Singh U., Jialal I. 2006. Oxidative stress and atherosclerosis. Pathophysiology;13: 129-42.
[4] Hairrudin, Dina H., and Yulestrina W. 2012. Aktivitas Fisik Berat Menyebabkan Degenerasi Sel Hepatosit melelui Mekanisme Stres Oksidatif. Jurnal kedokteran Brawijaya. 27: 61-65.
[5] Radak Z., Zhao Z., Koltai E. 2012. Oxygen Consumption and Usage During Physical Exercise: The Balance Between Oxidative Stress and ROS-Dependent Adaptive Signaling. Antioxidant and Redox Signaling. 18: 1208–1246
[6] Cooper, K., Baraas, Faisal, Wibowo, Singgih, M. 2001. Sehat Tanpa Obat : Empat Langkah Revolusi Antioksidan Yang Mengubah Hidup Anda. Bandung : Kaifa.
[7] Sugianto, Nanik Lidyawati. 2011. Pemberian Jus Delima Merah (Punica granatum) Dapat Meningkatkan Kadar Glutathion Peroksidase Darah Pada Mencit (Mus Musculus) dengan Aktivitas Fisik Maksimal [Tesis]. Denpasar : Universitas Udayana.
[8] Arsana, I.N. 2014. Ekstrak Kulit Buah Manggis (Garcinia mangostana L.) dan Pelatihan Fisik Menurunkan Stres Oksidatif Pada Tikus Wistar (Rattus norvegicus) Selama Aktivitas Fisik Maksimal [Dissertation]. Universitas Udayana.
[9] Junquiera, L.C., and Carneiro, K. 2005. Basic Histology Test and Atlas. Department of Cell and Developmental Biology. Institute of Biomedical Sciences. Sao Paolo. Edisi 11. Chapter 21.
[10] Maffetone. P. 2007. The Overtraining Syndrom. International Sport Sciences Association. Accesed : August, 13th 2015.
[11]. Mastorakos, G., Maria P., Evanthia D.K., Georga P.C. 2005. Exercise and The Stress System. Hormones. 4: 73-89.
[12] Spolaore, P., Joannis, C,. Duran, E dan Isambert, A. 2006. Commercial Applications of Microalgae. Journal of Bioscience and Bioenginering, Vol 101: 87-96 pp.
[13] El-Desoky, Gaber E., Samir A. Bashandy, Ibrahim M. Alhazza, Zeid A. Al-Othman, Mourad A. M. Aboud-Soud and Kareen Yusuf. 2013. Improvement of Mercuric Chloride-Induced Testis Injuries and Sperm Quality Deteriorations by Spirulina platensis in Rats. Public Library of Science. 8: 1-9.
[14] Ferreira-Hermosilo A, Torres-Durran PV, Shamosh-Halabe S, and Juarez-Oropeza MA. 2011. Biological Effect of Spirulina and Current Research on Its Antioxidant Activity. Toctli Revista Internacional de Ciencia y Technologia Biomedica. 2: 1-13.
[15]`Shaikh, H., VK Shrivastava, R. Shrivastava, M. Amir. Chemo-Protective Effect Of Turmeric And Spirulina On Cisplatin Induced Toxicity In Reproductive System Of Male Albino Rat, Rattusnorvegicus. Indo American Journal Of Pharmaceutical Research. 3 : 7257 - 7264
[16] Vijayprasad, Sanghishetti., Ghongane BB, and Nayak BB. 2014. Effect of Vitamin C on Male Fertility in Rats Subjected to Forced Swimming Stress. Journal of Clinical and Diagnostic Research. 8: 5-8.
[17] Hutadilok-Towatana, N., W. Reanmongkol, S. Satitit, P. Panichayupakaranant and P. Ritthisunthorn. 2008. A subchronic toxicity study of Spirulina platensis. Food Science Technology Research. 14: 351-358
[18] Aprilliani, M., Nurcahyani, N., Busman, H. 2013. Efek Pemaparan Kebisingan Terhadap Jumlah Sel-Sel Spermatogenik Dan Diameter Tubulus Seminiferus Mencit (Mus musculus L.). Seminar Nasional Sains & Teknologi V. Lampung, Indonesia.
[19] Johnson, L. and Thompson, Jr.D.L. 1986. Seasonal variation in the total volume of Leydig cells in stallion is explained by variation in cell number rather than cell size. Biology of Reproduction. 52: 971-79.
[20] Sonjaya, H. 2012. Dasar Fisiologi ternak. IPB Press. Bogor
[21] Siregar, J. H. 2009. Pengaruh Pemberian Vitamin C terhadap Jumlah Sel Leydig dan Jumlah Sperma Mencit Jantan Dewasa (Mus musculus L.) yang Dipapari Monosodium Glutamat (MSG) [Tesis]. Program Pasca Sarjana. Universitas Sumatra Utara. Medan.
[22] Wahyu R. 2016. Effect of Spirulina platensis On the Number of Spermatogenic Cells in the Seminiferous Tubules of Rat (Rattus norvegicus) With Excessive Physical Exercise [Thesis]. Universitas Airlangga. Surabaya
[23] Anindita dan Sutyarso. 2012. Pengaruh Pemberian Vitamin C Terhadap Berat Testis, Jumlah Sel Leydig, dan Diameter Tubulus Seminiferus Mencit (Mus musculus) Jantan Dewasa Yang Diinduksi Monosodium Glutamat. Jurnal Fakultas Kedokteran Universitas Lampung. 36-48.
