Published date: Mar 03 2023

Journal Title: KnE Social Sciences

Issue title: 2nd Paris Van Java International Seminar on Health, Economics, Social Science and Humanities (PVJ-ISHESSH 2021)

Pages: 772–780

DOI: 10.18502/kss.v8i4.12972

Hasni Yaturramadhan Harahaphyaturramadhan@gmail.comDepartment of Pharmacy, Faculty of Health, Universitas Aufa Royhan, Padangsidimpuan, Sumatera Utara, Indonesia.

Cory Linda FutriDepartment of Pharmacy, Faculty of Health, Universitas Aufa Royhan, Padangsidimpuan, Sumatera Utara, Indonesia.

Dalimunthe AminahDepartment of Pharmacology, Fakulty of Pharmacy, Universitas Sumatera Utara, Indonesia.

Widyawati TriDepartment of Pharmacology, Fakulty of Pharmacy, Universitas Sumatera Utara, Indonesia.

This study aimed to determine the effect of Dayak Union tuber (Eleutherine bulbosa (Mill) Urb) ethanol extract on the regeneration of pancreatic β cells of Wistar rats induced streptozotocin. This research is laboratory experimental study by using 30 rats, they are divided into 5 groups, each group consisting of 6 rats, they are group 1 (normal control), group 2 (sick control) was given Na CMC 1 % b/v, Group 3 (positive control) was given glibenclamide at a dose of 0.25 mg / KgBW, Group 4,5 and 6 were given (Eleutherine bulbosa (Mill) Urb) extract at a dose of 125 mg / KgBB, 250 mg / KgBB and 500 mg / kgBW orally for 15 consecutive days. The histopathological damage level of the pancreas was observed with HE staining using a 400x magnification Olympus Cx-21 microscope. The result of the study showed that there were secondary metabolites of alkaloids, flavonoids, saponins, steroids, and tannins in the ethanol extract of Eleutherine palmifolia (Mill) Urb); The histopathological description showed that the damage to the structure of Langerhans islands and only a dose of 500 mg / KgBB the ethanol extract of Eleutherine palmifolia (Mill) Urb) showed improvement.

Keywords: Dayak Onion Tuber; histopathological; extract ethanol

[1] Kusmiati M, Nurpalah R, Mardiana U, Nugraha RW. The effectiveness of “X” kefir brand towards glucose level on post prandial pre-diabetes male mice (Mus Musculus) Swiss Webster strains orally. Journal of Physics: Conference Series. IOP Publishing. 2019;12135.

[2] Babu PVA, Liu D, Gilbert ER. Recent advances in understanding the anti-diabetic actions of dietary flavonoids. The Journal of nutritional biochemistry. 2013;24(11):1777- 1789.

[3] American Diabetes Association (ADA). Herbs, supplements and alternative medicine. 2012. Diabetes.org. Available from:http://www. diabetes. org/ living - withdiabetes/ treatment - and care/ medication /herbs - supplements – alternative - medicines/.

[4] Aguilara FZA, Ramos RR, Guiterrez SP et al. Studi of antihyperglaicemic effect of plant used as anti diabetics. J. Page. 1998;101-110.

[5] Apriani N, Suhartono E, Izaak ZA et al. Korelasi kadar glukosa darah dengan kadar advanced oxidation protein produc (AOPP) Tulang pada tikus putih model hiperglikemia. 2011.

[6] American Diabetes Assosiacion (ADA). Diagnosis and classification of diabetes mellitus diabetes care USA. ADA; 2010.

[7] Ali RA, Dahiya A, Kushoor A, Rohilla. Streptozotocin induced diabetes mechanism of induction. International Journal of Pharmaceutical Antioxidan Activity Of Moringaoleifera. International Journal Molecular. 2011:6607-6088.

[8] Akbarzadeh A, Norouzian D, Mehrabi MR et al. Induction of diabetes by streptozotocin in rats. Indian Journal of Clinical Biochemistri. 2007;60-64.

[9] Dewi LK, Widyarti S, Rifai M. Pengaruh pemberian ekstrak etanol daun sirsak (annoramuricata linn) terhadap peningkatan jumlah sel cd4+ dan cd8+ pada timus mencit (musmulucus). Biology Journal. 2013.

[10] Ridwan A. Astrian, tanita, radendan barlian anggreani. pengukuran efek antidiabetes polifenol (Polihenon 60) berdasarkan kadar glukosa darah dan histologi pankreas mencit (Musmusculus L) S. W. Jantan Yang Dikondisikan Diabetes Mellitus. 2012.

[11] Tandi J, Roem M, Yuliet. Efek nefropati kombinasi ekstrak daun gedi merah dan kumis kucing pada tikus induksi etilen glikol. STIFA Pelita Mas. Jurnal Trop Pharmacy Cemistry. 2017;4(1):27-34.

[12] Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Poznan: University of Agriculture; 2017.

[13] Dewi NP, Allia R, Sabang SM. Uji efektivitas antidiabetes (Eleutherine bulbosa (MILL) URB. terhadap penurunan kadar glukosa darah tikus obesitas. Jurnal Farmasi, Sekolah Tinggi Farmasi Pelita Mas Palu (STIFA), Samarinda. 2016.