Published date: Jun 03 2022

Journal Title: KnE Medicine

Issue title: The 4th International Seminar on Global Health (ISGH4)

Pages: 143–150

DOI: 10.18502/kme.v2i2.11077

Arina Novillaarin_novilla@yahoo.co.idDepartment of Medical Laboratory Technology, Faculty of Health Science and Technology, Universitas Jenderal Achmad Yani Cimahi, Indonesia

Wikan MargahyaniDepartment of Medical Laboratory Technology, Faculty of Health Science and Technology, Universitas Jenderal Achmad Yani Cimahi, Indonesia

Dwi Davidson RihibihaDepartment of Medical Laboratory Technology, Faculty of Health Science and Technology, Universitas Jenderal Achmad Yani Cimahi, Indonesia

It has been reported that polyphenols-rich diets provide some protection against the development of cancers, cardiovascular diseases, diabetes, osteoporosis and neurodegenerative diseases. Exogenous antioxidants are abudantly found in plants. Tea (Camellia sinensis L.; family Theaceae), which is the most popular beverage in Southeast Asia, is known for its medicinal properties. In this study, we evaluated antioxidant activity as well as phenol and flavonoid content in green tea leaves from PPTK Gambung Ciwidey in West Java. Antioxidant activity was observed through measuring 2,2- diphenyl-1 picrylhydrazyl (DPPH) scavenging activity. The total phenolic content was determined by the Folin Ciocalteu method. The green tea leaf extract showed the highest DPPH-scavenging activity at a concentration of 25 ug/ml (DPPH = 94.55%;IC50 = 0.54 μg/mL). Furthermore, the total phenolic and flavonoid contents of the green tea leaf extract were 36.64 and 8.43 mg QE/mg extract, respectively. These findings showed that the green tea leaf extract exhibited antioxidant activity.

Keywords: Camellia sinensis L, flavonoid, antioxidant

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