Published date: Apr 05 2021

Journal Title: KnE Life Sciences

Issue title: DonAgro: International Research Conference on Challenges and Advances in Farming, Food Manufacturing, Agricultural Research and Education

Pages: 757–764

DOI: 10.18502/kls.v0i0.9013

Abdulmalik Batukaev batukaevmalik@mail.ruChechen State University; Chechen Research Institute of Agriculture, Grozny, Russia

Eliza SobralievaChechen Research Institute of Agriculture, Grozny, Russia

Diana PalaevaChechen Research Institute of Agriculture, Grozny, Russia

This article describes the effect of Gautheret, White, Heller and Murashige & Skoog mineral salts during in-vitro clonal micropropagation of new grape varieties. The optimal mineral compositions of the culture medium that support the in-vitro regeneration of isolated grape explants were identified. The grapes that were studied were the Bart and Augustine varieties. Primary grape explants were cultivated for 30 days in a nontransplanted culture. Increased regenerative activity was observed in the Murashige & Skoog and White media. Increased haemogenesis occurred and shoots regenerated. The addition of cytokinin 6-BAP to the medium for obtaining aseptic culture led to an increase in the frequency of shoot-bud production by 5 to 6 times, depending on the type of medium. Combining 6-BAP with the auxin NAA provided an additional increase in the frequency of shoot-bud production, but to a lesser extent. Adding growth regulators to the culture medium also reduced the frequency of explant necrosis.

Keywords: grapes, mineral salts, culture medium, microclonal propagation, in-vitro, cytokinins, auxins

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