Published date: Mar 15 2021

Journal Title: KnE Life Sciences

Issue title: The 4th International Virtual Conference on Nursing

Pages: 930-939

DOI: 10.18502/kls.v6i1.8770

Fatin Naziha Osmanfatinn9604@puncakalam.uitm.edu.myFaculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia

Fatin Nadzirah ZakariaFaculty of Health Sciences, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia

Siti Aishah ZulazlanFaculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia

Nooraain HashimFaculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia

Razif DasimanFaculty of Health Sciences, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia

Nina Keterina HashimFaculty of Health Sciences, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia

Oligomeric Proanthocyanidins (OPC) is a type of polyphenolic compound which have been demonstrated to have anti-cancer, anti-oxidant, anti-inflammatory and anti-mutagenic properties that may have the potential to reduce intergenerational effect of BPA towards DNA. This study was conducted to determine the effect of OPC on the DNA damage of BPA-induced rats using microarray CGH Chromosome Karyotyping. Adult Male Sprague Dawley rats were divided into six groups which are Normal, BPA, OPC10, OPC20, BPA+OPC10 and BPA+OPC20. The administration of BPA and/or OPC were conducted for 21 days using oral gavage before being mated with female rats of the same age at 1:1 mating ratio. Once the female rats were confirmed pregnant, the male rats were decapitated and their blood were collected for chromosome microarray analysis. The male offspring (F1 generation) were allowed to grow until 10 weeks old and their blood were also collected for chromosome microarray analysis. BPA group had a deletion of Vomeronasal receptor genes in which the deletion magnitude increased from P to F1 generation may affect the ability of the rats to detect chemosensory cues during neurobehavioral test. The amplification of Major Histocompatibility complex (MHC) class I gene in BPA+OPC20 group may aid in a better performance during hippocampal-dependent memory tests. These results suggested that OPC could be a potential agent in reducing the intergenerational effect of BPA. Current finding may enrich our understanding particularly in the possible mechanism of OPC on BPA-induced memory impairment

Keywords: Bisphenol-A, Brain, microarray CGH Chromosome Karyotyping, Oligomeric Proanthocyanidins, Intergenerational

[1] Hanson, M. A. and Skinner, M. K. (2016). Developmental Origins of Epigenetic Transgenerational Inheritance. Environmental Epigenetics. Vol. 2, issue 1, pp. 1–21. DOI: 10.1093/eep/dvw002


[2] Fernández-santiago, R. and Ezquerra, M. (2016). Epigenetic Research of Neurodegenerative Disorders using Patient Ipsc-Based Models. Stem Cells International. Vol 2016. DOI: 10.1155/2016/9464591


[3] Haque, M. M., et al. (2016). Genomic Clustering of Differential DNA Methylated Regions (Epimutations) Associated with the Epigenetic Transgenerational Inheritance of Disease and Phenotypic Variation. BMC Genomics., pp. 1–13.


[4] Inadera, H. (2015). Neurological Effects of Bisphenol A and its Analogues. International Journal Of Medicine sciences., vol. 12, issue 12, p. 926.


[5] Liu, Y., et al. (2013). Secondary Metabolites in Durian Seeds: Oligomeric Proanthocyanidins Molecules. Vol. 18, issue 11, pp. 14172–85.


[6] Khanbabaee, K. and Ree, T. (2001). Tannins: Classification and Definition. Nat Prod Rep., vol. 18, issue 6, pp. 641–9.


[7] Beaudet, A. L. (2014). The Utility of Chromosomal Microarray Analysis in Developmental and Behavioral Pediatrics. Child Dev., vol. 84, issue 1, pp. 1–20.


[8] Theisen, A. (2008). Microarray-Based Comparative Genomic Hybridization (aCGH). Nat Educ., vol. 1, issue 1, p. 45.


[9] Gullotta, F., et al. (2007). Prenatal Diagnosis of Genomic Disorders and Chromosome Abnormalities using Array-Based Comparative Genomic Hybridization. Journal of Perinatal Medicine., vol. 1, issue 1, pp. 16–22.


[10] (2013). Unique. Microarray-Based Comparative Genomic Hybridisation (array CGH). Underst Chromosom Disord., issue 12.


[11] Bahari, E. A., et al. (2016). The Effects of Phyllanthus Gomphocarpus Hook. F. Aqueous Extract Supplementation on Androgenic Hormonal Level and Histological Testes Morphometry. J Teknol., vol. 78, issue 6–7.


[12] Zhang, X., Marcucci, F. and Firestein, S. (2010). High-Throughput Microarray Detection of Vomeronasal Receptor Gene Expression in Rodents. Frontiers in Neuroscience., issue 4, pp. 1–20.


[13] Ibarra-Soria, X., Levitin, M. O. and Logan, D. W. (2014). The Genomic Basis of Vomeronasal-Mediated Behaviour. Mamm Genome, vol. 25, issue 1–2, pp. 75–86.


[14] Martinez-Arguelles, D. B. and Papadopoulos, V. (2015). Identification of Hot Spots of DNA Methylation in the Adult Male Adrenal in Response to in Utero Exposure to the Ubiquitous Endocrine Disruptor Plasticizer Di-(2-Ethylhexyl) Phthalate. Endocrinology, vol. 156, issue 1, pp. 124–33.


[15] Nelson, P. A., et al. (2013). MHC Class I Immune Proteins are Critical for Hippocampus-Dependent Memory and Gate NMDAR-Dependent Hippocampal Long-Term Depression. Learning and Memory., vol. 20, issue 9, pp. 505–17.


[16] Lazarczyk, M. J., et al. (2016). Major Histocompatibility Complex Class I Proteins are Critical for Maintaining Neuronal Structural Complexity in the Aging Brain. Scientific Reports., vol. 6, p. 26199.


[17] Margarit, T. and Lancet, D. (1993). Expression of Olfactory Receptor and Transduction Genes During Rat Development. Dev Brain Res., vol. 73, issue 1, pp. 7–16.


[18] Brann, J. H. and Firestein, S. J. (2014). A Lifetime of Neurogenesis in the Olfactory System. Frontiers in Neuroscience., issue 8, p. 182.