KnE Life Sciences

ISSN: 2413-0877

The latest conference proceedings on life sciences, medicine and pharmacology.

Mus musculus (Linnaeus, 1758) Immune Responses Caused by Escherichia coli (Migula, 1895) Infection

Published date: Jul 11 2017

Journal Title: KnE Life Sciences

Issue title: The 4th International Conference on Biological Science (2015)

Pages: 285-293

DOI: 10.18502/kls.v3i4.716

Authors:
Abstract:

Escherichia coli (Migula, 1895) is a negative gram bacteria which have an ability to cause some deseases, such as meningitis, urinary tract infection and digestive tract infection. Lipopolysaccharide (LPS) in its cell wall induces immune responses in many ways. The objective of this study was to investigate the effect of E. coli infection on lymphocyte numbers and spleen weight in mice [Mus musculus (Linnaeus, 1758)]. Twenty five male mice were grouped into negative control, positive control group treated with PBS, and three experimental groups injected intraperitonially with E. coli 1.5 × 103 cfu/mL,1.5 × 105 cfu/mL or 1.5 × 107 cfu/mL respectively.  About 5 d after the injection, the spleen were collected. Spleen were weighed using digital balance, number of lymphocyte were counted using hemocytometer. E. coli infection significantly increase both weight of the spleen and number lymphocyte. In conclusion,  mice responded to E. coli infection by increasing the number of its lymphocyte.

 

Keywords: Escerechia coli (Migula, 1895); immune respone; infection; Mus musculus (Linnaeus, 1758).

References:

[1] RG. Hamilton, The human IgG subclasses, Calbiochem, Baltimore, 2001.

[2] P. Saroj, M. Verma, KK. Jha, and M. Pal, “An overview on immunomodulation,” Journal of Advanced Scientific Research, p. 12, 2012.

[3] A. Martner, “Regulation of innate and adaptive immune responses by grampositive and gram negative bacteria,” in [Online]. ( accessed Januari 31st, URL https, //gupea.ub.gu.se/bitstream/2077/20049/1/gupea_2077_20049_1.pdf, 2015, https://gupea.ub.gu.se/bitstream/2077/20049/1/gupea_2077_20049_1.pdf.

[4] O. Tenaillon, D. Skurnik, B. Picard, and E. Denamur, “The population genetics of commensal Escherichia coli,” Nature Reviews Microbiology, vol. 8, no. 3, pp. 207– 217, 2010.

[5] J. B. Kaper, J. P. Nataro, and H. L. T. Mobley, “Pathogenic Escherichia coli,” Nature Reviews Microbiology, vol. 2, no. 2, pp. 123–140, 2004.

[6] CPD. Sousa, “Escherichia coli as a specialized bacterial pathogen,” Journal of Revista De Biologia E Ciencias Da Terra, pp. 341–352, 2006.

[7] J. C. Marshall, D. Foster, J.-L. Vincent et al., “Diagnostic and prognostic implications of endotoxemia in critical illness: Results of the MEDIC study,” Journal of Infectious Diseases, vol. 190, no. 3, pp. 527–534, 2004.

[8] MRG. OGorman and AD. Donnenberg, “Handbook of human immunology, Second Edition,” in O’Gorman MRG, p. 1, CRC Press, Newyork, 2008.

[9] E. Kurnianingtyas, M. S. Djati, and M. Rifa’i, “Aktivitas Imunomodulator Polyscias obtusa Terhadap Sistem Imunitas Pada Bone Marrow Broiler Setelah Pemberian Salmonella typhimurium,” The Journal of Experimental Life Sciences, vol. 3, no. 1, pp. 25–30, 2013.

[10] AC. Guyton and JE. Hall, “Medical physiology,” in Medical physiology, pp. 440–441, Elsivier Inc, Pennsylvania, 11th edition, 2006.

[11] P. Mitra Mazumder, S. Pattnayak, H. Parvani, D. Sasmal, and P. Rathinavelusamy, “Evaluation of immunomodulatory activity of Glycyrhiza glabra L roots in combination with zing,” Asian Pacific Journal of Tropical Biomedicine, vol. 2, no. 1, pp. S15–S20, 2012.

[12] R. E. Mebius and G. Kraal, “Structure and function of the spleen,” Nature Reviews Immunology, vol. 5, no. 8, pp. 606–616, 2005.

[13] B. Steiniger, “Spleen,” in Encyclopedia of Life Sciences, p. 19, Encyclopedia of Life Sciences, 2005.

[14] SB. Kresno, “Imunologi, diagnosis dan prosedur laboratorium edisi kelima [Immunology: diagnosis and laboratory procedures fifth edition],” in n Penerbit Fakultas Kedokteran Universitas Indonesia, p. 74, Penerbit Fakultas Kedokteran Universitas Indonesia, Jakarta, 2013, 78 in Bahasa Indonesia.

[15] A. Goraca, H. Huk-Kolega, P. Kleniewska, A. Piechota-Polańczyk, and B. Skibska, “Effects of lipoic acid on spleen oxidative stress after LPS administration,” Pharmacological Reports, vol. 65, no. 1, pp. 179–186, 2013.

[16] M. A. E. Dkhil, “Apoptotic changes induced in mice splenic tissue due to malaria infection,” Journal of Microbiology, Immunology and Infection, vol. 42, no. 1, pp. 13– 18, 2009.

[17] W. J. Hopkins, A. Gendron-Fitzpatrick, E. Balish, and D. T. Uehling, “Time course and host responses to Escherichia coli urinary tract infection in genetically distinct mouse strains,” Infection and Immunity, vol. 66, no. 6, pp. 2798–2802, 1998.

[18] AG. Garido, de. Poli Figueiredo LF, and ESM. Rocha, “Experimental models of sepsis: An overview. Acta Cirurgica Brasileira,” in Rocha ESM. Experimental models of sepsis: An overview. Acta Cirurgica Brasileira, pp. 19–82, 19, 82–88, 2004.

[19] J. P. McAleer and A. T. Vella, “Understanding how lipopolysaccharide impacts CD4 T-cell immunity,” Critical Reviews in Immunology, vol. 28, no. 4, pp. 281–299, 2008.

[20] Z.-F. Li, S. Zhang, G.-B. Lv et al., “Changes in count and function of splenic lymphocytes from patients with portal hypertension,” World Journal of Gastroenterology, vol. 14, no. 15, pp. 2377–2382, 2008.

[21] QH. Kshash, FG. Habasha, and SK. Al-Rammahi, “Experimental study on the role of purified LPS of E. coli O111:B4 in preventing mammary gland infection in mice,” Iraqi Journal of Veterinary Science, pp. 23–223, 2009.

[22] P. Wong and E. G. Pamer, “Cutting edge: Antigen-independent CD8 T cell proliferation,” Journal of Immunology, vol. 166, no. 10, pp. 5864–5868, 2001.

[23] A. Ayala, C. D. Herdon, D. L. Lehman, C. M. DeMaso, C. A. Ayala, and I. H. Chaudry, “The induction of accelerated thymic programmed cell death during polymicrobial sepsis: Control by corticosteroids but not tumor necrosis factor,” Shock, vol. 3, no. 4, pp. 259–267, 1995.

[24] R. S. Hotchkiss, K. W. Tinsley, P. E. Swanson et al., “Prevention of lymphocyte cell death in sepsis improves survival in mice,” Proceedings of the National Academy of Sciences of the United States of America, vol. 96, no. 25, pp. 14541–14546, 1999.

[25] R. S. Hotchkiss, P. E. Swanson, B. D. Freeman et al., “Apoptotic cell death in patients with sepsis, shock, and multiple organ dysfunction,” Critical Care Medicine, vol. 27, no. 7, pp. 1230–1251, 1999.

[26] S. B. Bradfute, D. R. Braun, J. D. Shamblin et al., “Lymphocyte death in a mouse model of ebola virus infection,” Journal of Infectious Diseases, vol. 196, no. 2, pp. S296–S304, 2007.

[27] R. S. Hotchkiss, P. E. Swanson, J. P. Cobb, A. Jacobson, T. G. Buchman, and I. E. Karl, “Apoptosis in lymphoid and parenchymal cells during sepsis: Findings in normal and T- and B-celldeficient mice,” Critical Care Medicine, vol. 25, no. 8, pp. 1298–1307, 1997

[28] AA. Yousif, NA. Al-Taai, and NM. Mahmood, “Humoral and cellular immune response induced by E. coli [O157:H7 and O157:K99] vaccines in mice,” in Mahmood NM. Humoral and cellular immune response induced by E. coli [O157:H7 and O157:K99] vaccines in mice. International Journal of Immunology Research, pp. 17–20, 2013.

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