Published date: Jul 11 2017

Journal Title: KnE Life Sciences

Issue title: The 4th International Conference on Biological Science (2015)

Pages: 241-246

DOI: 10.18502/kls.v3i4.711

Sri Winarniwiwin.undip@gmail.com

Yudhy Dharmawan

“Koro benguk” [Mucuna pruriens (L.) DC] seed contains L-dopa which is able to repair spermatozoa quality.  The aim of this experiment is to analyze L-Dopa using HPLC in engineering manufacture of tempeh benguk. There are four engineering model’s variants of making tempeh benguk. The engineering process begins with selection of koro benguk seed, tools required, immersion, boiling, humidity, temperature and pH. The result shows that the process in making tempeh benguk which contains the highest concentration of L-Dopa (11 346.85 mg · kg^–1) is white-colored  and intact koro benguk seed; one time immersion with replacing the water for 4  to 5 times/day (two days and two nights); one time boiling (< 1 h  until koro benguk seed can be opened); mixed with 10 mL lime juice/1.5 kg, with concentration of 0.83 % until pH 5, added with mashed teak leaves, and ended with the process of making tempeh benguk at room temperature (30 ºC), relative humidity room (65 %). Tempeh benguk which is produced is cleaner, whiter, nonperishable, and more tender.

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