Published date: Jul 11 2017

Journal Title: KnE Life Sciences

Issue title: The 4th International Conference on Biological Science (2015)

Pages: 201-207

DOI: 10.18502/kls.v3i4.705

Rini Riffianiriniriffiani@gmail.com

Nunik Sulistinah

Bambang Sunarko

This paper describes a efficient screening gene nitrilase from contaminated soil from Lombok gold mine industry. DNA was extracted directly from soil using the soil DNA isolation kit based on enzymatic, chemical and mechanical lysis. The existence of nitrilase gene in soil sample can be identified by nitrilase gene amplification using H1F˗H1R primer. BLASTN analysis result revealed that the nitrilase gene fragment which was amplified by H1F˗H1R primer has a high homology with Rhodococcus rhodochrous strain tg1˗A6 nitrilase gene. These amplification and DNA fragment sequencing results indicated that nitrilase gene existence on soil sample can be identified by metagenomic approach

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