Published date: Jul 11 2017

Journal Title: KnE Life Sciences

Issue title: The 4th International Conference on Biological Science (2015)

Pages: 170-176

DOI: 10.18502/kls.v3i4.701

Nur Laililie_azzahra@yahoo.co.id

Sarjiya Antonius

Bacillus sp. being industrially important organisms produces a wide variety of extracellular enzymes including protease. Bacillus sp. 140-B isolated from rhizosphere area of pineapple plantation in Lampung Province, Indonesia was tested for production of protease on skim milk agar medium. The aims of this study were to characterize and investigate some properties of  protease activity from Bacillus sp. 140-B. Bacillus sp. 140-B showed  protease activity qualitatively by clear zone diameter of 15.0 mm and the highest protease activity was 35.02 Unit · mg^–1 protein at 14 h after incubation. Some properties of protease activities from Bacillus sp. 140-B including effects of temperature, pH and several metals were observed in this experiment. The protease activity from Bacillus sp. 140-B had optimum pH of 7,0 and the optimum temperature was 60 °C. Several metals which were evaluated on protease activity showed that Mn could increase protease activity, while other metals (Ca, Co, and Hg) showed decreasing protease activity of Bacillus sp. 140-B.

[1] W. C. A. Do Nascimento and M. L. Leal Martins, “Production and properties of an extracellular protease from thermophilic Bacillus sp,” Brazilian Journal of Microbiology, vol. 35, no. 1-2, pp. 91–96, 2004.

[2] W. Chouyyok, N. Wongmongkol, N. Siwarungson, and S. Prichanont, “Extraction of alkaline protease using an aqueous two-phase system from cell free Bacillus subtilis TISTR 25 fermentation broth,” Process Biochemistry, vol. 40, no. 11, pp. 3514–3518,2005.

[3] C. Muthulaksmi, D. Gomathi, DG. Kumar, G. Ravikumar, M. Kalaiselvi, and C. Uma, “purification and characterization of protease by Aspergillus flavus under solid state fermentation,” Jordan Journal of Biological Sciences, vol. 4, pp. 137–148, 2011.

[4] R. Gupta, Q. Beg, and P. Lorenz, “Bacterial alkaline proteases: Molecular approaches and industrial applications,” Applied Microbiology and Biotechnology, vol. 59, no. 1, pp. 15–32, 2002.

[5] B. Sana, D. Ghosh, M. Saha, and J. Mukherjee, “Purification and characterization of a salt, solvent, detergent and bleach tolerant protease from a new gammaProteobacterium isolated from the marine environment of the Sundarbans,” Process Biochemistry, vol. 41, no. 1, pp. 208–215, 2006.

[6] G. Huang, T. Ying, P. Huo, and J. Jiang, “Purification and characterization of a protease from Thermophilic bacillus strain HS08,” African Journal of Biotechnology, vol. 5, no. 24, pp. 2433–2438, 2006.

[7] H. Joo, “Purification and Characterization of a Novel Alkaline Protease from Bacillus horikoshii,” Journal of Microbiology and Biotechnology, vol. 22, no. 1, pp. 58–68, 2012.

[8] Q. K. Beg, V. Sahai, and R. Gupta, “Statistical media optimization and alkaline protease production from Bacillus mojavensis in a bioreactor,” Process Biochemistry, vol. 39, no. 2, pp. 203–209, 2003.

[9] R. M. Banik and M. Prakash, “Laundry detergent compatibility of the alkaline protease from Bacillus cereus,” Microbiological Research, vol. 159, no. 2, pp. 135–140, 2004.

[10] A. Gerze, D. Omay, and Y. Guvenilir, “Partial purification and characterization of protease enzyme from Bacillus subtilis megatherium,” Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, vol. 121, no. 1-3, pp. 335–345, 2005.

[11] V. F. Soares, L. R. Castilho, E. P. S. Bon, and D. M. G. Freire, “High-yield Bacillus subtilis protease production by solid-state fermentation,” Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, vol. 121, no. 1-3, pp. 311–319, 2005.

[12] F. M. Olajuyigbe and J. O. Ajele, “Thermostable alkaline protease from bacillus licheniformis lbbl-11 isolated from traditionally fermented african locust bean (parkia biglobosa),” Journal of Food Biochemistry, vol. 35, no. 1, pp. 1–10, 2011.

[13] K. Mohammed and W. R. Pramod, “Production optimization of an extracellular coldactive alkaline protease from Stenotrophomonas maltophilia MTCC 7528 and its application in detergent industry,” African Journal of Microbiology Research, vol. 5, no. 7, pp. 809–816, 2011.

[14] P. Shivanand and G. Jayaraman, “Isolation and characterization of a metal iondependent alkaline protease from a halotolerant Bacillus aquimaris VITP4,” Indian Journal of Biochemistry and Biophysics, vol. 48, no. 2, pp. 95–100, 2011.

[15] N. Sevinc and E. Demirkan, “Production of protease by Bacillus sp. N-40 isolated from soil and its enzymatic properties,” Journal of Biology Environmental Sciences, vol. 5, pp. 95–103, 2011.