Published date: Jul 11 2017

Journal Title: KnE Life Sciences

Issue title: The 4th International Conference on Biological Science (2015)

Pages: 147-156

DOI: 10.18502/kls.v3i4.699

Ni Wayan Sri Agustiniwayan_sa2002@yahoo.com

Kusmiati .

Sources of antioxidants are abundant in nature, one of which is derived from microalgae. Microalgae species that have potentially to be developed as a producer of antioxidant compounds is Porphyridium cruentum (S.F.Gray) Nägeli. P. cruentum is a red microalga (Rhodophyceae) and has been known as polysaccharides producer (exopolysaccharide and endopolysaccharide) and as an antioxidant. This study aims to determine antioxidant activity of the extracts and biological toxicity of endo- and exopolysaccharide P. cruentum. The antioxidant activity use reduction method of free radicals (DPPH), whereas toxicity uses Brine Shrimp Lethality Test. (BSLT). The result shows that there is an antioxidant activity with IC₅₀ value of 50.586 5 mg · kg^–1 (exopolysaccharide) and 145.998 8 mg · kg^–1 (endopolysaccharide). In addition, the result indicates the toxic nature of the Artemia salina Leach. with LC₅₀ values of 513.175 1 mg · kg^–1 (exopolysaccharide) and 521.823 3 mg · kg^–1  (endopolysaccharide). Therefore, endo- and exopolysaccharide produced by P. cruentum suitable to be used as an alternative source of natural antioxidants and potential for further developed as antitumor drugs.

[1] H. Winarsi, “Antioksidan alami dan radikal bebas,” in Antioksidan alami dan radikal bebas, V. and dan. Antioksidan alami radikal bebas, Eds., vol. 12, 1518, 78, p. 11, Kanisius, Yogyakarta, 2007, in Bahasa Indonesia.

[2] Praptiwi Sr., P. Dewi, and M. Harapini, “Nilai peroksida dan aktivitas anti radikal bebas diphenyl picril hydrazil (DPPH) ekstrak metanol Knema laurina [Peroxide value and anti-free radical activity of diphenyl picril hydrazil (DPPH) Knema Laurina methanol extract],” Majalah Farmasi Indonesia, vol. 17, pp. 32–36, 2006, in Bahasa
Indonesia.

[3] Tamat Sr., T. Wifanto, and LS. Maulina, “Aktivitas antioksidan dan toksisitas senyawa bioaktif dari ekstrak rumput laut hijau Ulva reticulate Forsskal [The antioxidant activity and toxicity of bioactive compounds from the extracts of green seaweed Ulva reticulate Forsskal],” Jurnal Ilmu Kefarmasian Indonesia, vol. 5, pp.
31–36, 2007, in Bahasa Indonesia.

[4] INK. Kabinawa, “Mikroalga sebagai sumber daya hayati (SDH) perairan dalam perspektif bioteknologi, dalam: Pidato Pengukuhan Ahli Penelitian Utama Khususnya Mikroalga; 2001 [Microalgae as aquatic biological resources in the perspective of biotechnology]. Bogor: Puslitbang Bioteknologi Lembaga Ilmu Pengetahuan
Indonesia: 2001. p. 5. [in Bahasa Indonesia]”.

[5] B. Chen, W. You, J. Huang, Y. Yu, and W. Chen, “Isolation and antioxidant property of the extracellular polysaccharide from Rhodella reticulata,” World Journal of Microbiology and Biotechnology, vol. 26, no. 5, pp. 833–840, 2010.

[6] T. Tannin-Spitz, M. Bergman, D. Van-Moppes, S. Grossman, and S. Arad, “Antioxidant activity of the polysaccharide of the red microalga Porphyridium sp.,” Journal of Applied Phycology, vol. 17, no. 3, pp. 215–222, 2005.

[7] B. N. Meyer, N. R. Ferrigni, and J. E. Putnam, “Brine shrimp: A convenient general bioassay for active plant constituents,” Planta Medica, vol. 45, no. 1, pp. 31–34, 1982.

[8] R. Djamil and T. Anelia, “Penapisan fitokimia, uji BSLT dan uji antioksidan ekstrak metanol beberapa spesies Papilionaceae [Phytochemical screening, BSLT test and antioxidants test of methanol extract of Papilionaceae species],” Jurnal Ilmu Kefarmasian Indonesia, vol. 7, no. 2, pp. 65–71, 2009, in Bahasa Indonesia.

[9] E. Percival and R. A. J. Foyle, “The extracellular polysaccharides of porphyridium cruentum and porphyridium aerugineum,” Carbohydrate Research, vol. 72, no. C, pp. 165–176, 1979