Published date: Jul 11 2017

Journal Title: KnE Life Sciences

Issue title: The 4th International Conference on Biological Science (2015)

Pages: 28-35

DOI: 10.18502/kls.v3i4.684

Andi Lindhemuthianingrum SiradjeMuthiasiradje@yahoo.com

Irfan D. Prijambada

Endah Retnaningrum

Pseudomonas geniculata has been isolated from uncontaminated vertisol in Kulon Progo district. The isolate is hydrocarbonoclastic bacterium capable of forming biofilm on the fungal hyphae. Sinergy of both microbe in the form fungal-bacteria biofilm produce high ability to degrading hydrocarbon and survive in its pollution environment. The purpose of this research was to evaluate ability of Pseudomonas geniculata (Wright, 1895) Chester, 1901 to form biofilm and its attachment on three fungals species such as Penicillium sp., Penicillium funiculosum and Penicillium crustosum. The diameter size of fungal hyphae was of 1.3 µm, 1.9 µm and 2.4 µm, respectively. P. geniculata required at least 48 h to form biofilms on Penicillium sp. hyphae when incubated in mineral Bushnell Haas Medium suplemented with 2 % glucose at room temperature, with maximal biofilm formation being evident at 360 h. Biofilm attachment on Penicillium sp. hyphae was disrupted by the vortex power of 5 rpm for 20 s. Interaction of P. geniculata and Penicillium sp. that has a smallest diameter size of hypha were more successful on biofilm formation and attachment which could contribute to bacterial survival in environmental stresses. 

[1] M. E. Davey, N. C. Caiazza, and G. A. O’Toole, “Rhamnolipid surfactant production affects biofilm architecture in Pseudomonas aeruginosa PAO1,” Journal of Bacteriology, vol. 185, no. 3, pp. 1027–1036, 2003.

[2] G. Seneviratne, J. S. Zavahir, W. M. M. S. Bandara, and M. L. M. A. W. Weerasekara, “Fungal-bacterial biofilms: Their development for novel biotechnological applications,” World Journal of Microbiology and Biotechnology, vol. 24, no. 6, pp. 739–743,2008.

[3] K. T. Elvers, K. Leeming, and H. M. Lappin-Scott, “Binary and mixed population biofilms: Time-lapse image analysis and disinfection with biocides,” Journal of Industrial Microbiology and Biotechnology, vol. 29, no. 6, pp. 331–338, 2002.

[4] L. Yang, Y. Liu, H. Wu, N. Høiby, S. Molin, and Z.-J. Song, “Current understanding of multispecies biofilms,” International Journal of Oral Science, vol. 3, no. 2, pp. 74–81, 2011.

[5] HMLI. Herath, DMN. Senanayeke, G. Seneviratne, and DC. Bandara, Variation of biochemical Expressions of developed fungal-bacterial biofilms over their monocultures and its effect on plant growth, Tropical Agricultural Research, 2013.

[6] K. T. Elvers, K. Leeming, C. P. Moore, and H. M. Lappin-Scott, “Bacterial-fungal biofilms in flowing water photo-processing tanks,” Journal of Applied Microbiology, vol. 84, no. 4, pp. 607–618, 1998.

[7] D. Utami, Kemampuan degradasi hidrokarbon minyak bumi oleh co-culture kapang dan bakteri dalam bentuk biofilm. Petroleum hydrocarbon degradation ability by co-culturing of fungi and bacteria in biofilm form. Undergraduate Thesis. Fakultas Pertanian, Universitas Gadjah Mada, in Bahasa Indonesia,.

[8] AY. Mahardhika, “Biodegradasi minyak nabati oleh jamur dan bakteri dalam bentuk biofilm. [Vegetable oil biodegradation by fungi and bacteria in biofilm form]. [Undergraduate Thesis], Fakultas Pertanian,” Universitas Gadjah Mada, 2015, in Bahasa Indonesia.

[9] P. Frey-Klett, P. Burlinson, A. Deveau, M. Barret, M. Tarkka, and A. Sarniguet, “Bacterial-fungal interactions: Hyphens between agricultural, clinical, environmental, and food microbiologists,” Microbiology and Molecular Biology Reviews, vol. 75, no. 4, pp. 583–609, 2011.

[10] D. A. Hogan and R. Kolter, “Pseudomonas-Candida interactions: An ecological role for virulence factors,” Science, vol. 296, no. 5576, pp. 2229–2232, 2002.

[11] DA. Hogan, MJ. Wargo, and N. Beck, “Bacterial biofilms on fungal surfaces,” in The biofilm mode of life: Mechanisms and adaptations. Horizon Scientific Press, Kjelleberg. and M. Givskov, Eds., pp. 235–245S, Horizon Scientific Press, Norfolk, United Kingdom, 2007.