Published date:Feb 11 2020

Journal Title: KnE Life Sciences

Issue title: The 2019 International Conference on Biotechnology and Life Sciences (IC-BIOLIS)

DOI: 10.18502/kls.v5i2.6446

Titta NoviantiBiotechnology Department, Faculty of Health Sciences, Universitas Esa Unggul, Jakarta

Muhammad Panji Januarsyah KustiawanBiotechnology Department, Faculty of Health Sciences, Universitas Esa Unggul, Jakarta

Blandina Ivanka Pinkan ManimpurungBiotechnology Department, Faculty of Health Sciences, Universitas Esa Unggul, Jakarta

Muhammad IchsanBiotechnology Department, Faculty of Health Sciences, Universitas Esa Unggul, Jakarta

Alfero Putra IriyantoBiotechnology Department, Faculty of Health Sciences, Universitas Esa Unggul, Jakarta

Febriana Dwi WahyuniBiotechnology Department, Faculty of Health Sciences, Universitas Esa Unggul, Jakarta

It JamilahBiology Department, Faculty of Mathematic and Sciences, Universitas Sumatera Utara, Medan

Syafruddin IlyasSyafruddin6@usu.ac.idBiology Department, Faculty of Mathematic and Sciences, Universitas Sumatera Utara, Medan

Mice can regenerate tissue in the third phalanges of digit tip after amputation. The tissue regeneration process involved various cells, molecules, proteins, and genes that were thought to have a role in the process. Hedgehog (Hh) gene was thought to have a role in tissue regeneration, but research in adult tissue regeneration was limited. Hh gene had a role in mitosis and cell morphogenesis during the embryo growth. The expression of this gene continued in the adult cell population. We analyzed the Hh gene expression in tissue regeneration of the third phalange digit tip mice post-amputation until day 25. We used 30 males of mice (Mus musculus var Webster) as an animal model. We observed the histological analysis and Hh gene expression in each growth day. Spearman correlation test results in a strong and significant correlation between the growth length of digit tip mice and mRNA expression of the Hh gene (p <0.005; r= 0.874).

Keywords: Hedgehog, tissue regeneration, digit tip, proliferation,

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