Published date: Feb 11 2020

Journal Title: KnE Life Sciences

Issue title: The 2019 International Conference on Biotechnology and Life Sciences (IC-BIOLIS)

Pages:

DOI: 10.18502/kls.v5i2.6445

Gili HeriawanDepartment of Biotechnology, School of Life Science, Indonesia International Institute for Life Sciences, Jalan Pulomas Barat Kav. 88, Kayu Putih, Pulo Gadung, Jakarta Timur

Dellamartha AnjaniDepartment of Biotechnology, School of Life Science, Indonesia International Institute for Life Sciences, Jalan Pulomas Barat Kav. 88, Kayu Putih, Pulo Gadung, Jakarta Timur

Putu Virgina Partha DevanthiDepartment of Biotechnology, School of Life Science, Indonesia International Institute for Life Sciences, Jalan Pulomas Barat Kav. 88, Kayu Putih, Pulo Gadung, Jakarta Timur

Rahmat Azhari KemalDepartment of Medical Biology, Faculty of Medicine, Universitas Riau, Jl Diponegoro no. 1, Pekanbaru, Riau, 28133

Priscilla Jessicapriscilla.jessica@i3l.ac.idDepartment of Biotechnology, School of Life Science, Indonesia International Institute for Life Sciences, Jalan Pulomas Barat Kav. 88, Kayu Putih, Pulo Gadung, Jakarta Timur

Recent studies have reported the presence of inulin, a prebiotic polysaccharide, in gembili (Dioscorea esculenta). Sucrose:sucrose 1-fructosyltransferase (1-SST) is an enzyme that catalyzes the first step of inulin biosynthesis. The identification of this enzyme would be the foundation to improve the yield of inulin in gembili; to modify 1-SST gene for this purpose, its sequence must first be determined. This study aimed to design primers to isolate 1-SST gene from D. esculenta. The primers were designed by using the whole-genome sequence of Dioscorea rotundata due to the lack of genomic information on D. esculenta. Sequences from chromosome 6 and 11 were used as the template of primer design in which four pairs of primers were selected. Amplification products with expected size were gel-purified, then the targets were sequenced and analyzed in-silico. As a result, one of the primer pairs had successfully isolated vacuolar invertase gene, which is closely related to 1-SST gene. On the other hand, the other primer pairs showed either negative or false-positive result. Using the current strategy, 1-SST gene sequence from D. esculenta has not been successfully isolated, thus other approaches in primer design should be considered in further studies.

Keywords: 1-SST, Dioscorea esculenta, Gembili, Inulin, Primer design

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