Published date:Feb 11 2020

Journal Title: KnE Life Sciences

Issue title: The 2019 International Conference on Biotechnology and Life Sciences (IC-BIOLIS)

DOI: 10.18502/kls.v5i2.6441

Ida Bagus Made ArtadanaFaculty of Biotechnology, University of Surabaya

Aurelia Nadine HandoyoFaculty of Biotechnology, University of Surabaya

Popy H. HardjoFaculty of Biotechnology, University of Surabaya

Maria Goretti PurwantoMaria@Staff.ubaya.ac.idFaculty of Biotechnology, University of Surabaya

Barak Cenana (Oryza sativa L cv. Barak Cenana) is a native red rice cultivar of Tabanan regency, Bali. We have previously created first-generation putative mutants (M1) lines of Barak Cenana using EMS. In this study, we aim to evaluate M1 lines tolerance to salt stress. Three-leaf seedlings of WT Barak Cenana and M1 lines were cultured in hydroponic system containing Yoshida solution and 100 mM NaCl for 7 days. The salt tolerance level of each seedling was evaluated using SES standard and the survived seedlings were transferred into the pot-containing soil growth for maturation. All WT were died when treated with 100 mM NaCl for 7 days. In contrast, some M1 lines were survived where 0.5% were highly tolerance (II2A-4) and 4.06% (1F-4, 1F-3, II2A-8, IID-1, IID-4, IID-6 and 1B-6) were tolerance to salt stress. This variation of salt tolerance level among M1 lines is likely due to the random mutation caused by EMS. Furthermore, all survived mutants were fertile and able to produce mature seeds. As characters in M1 generation are not stable, future studies are required to establish stable mutant lines.

Keywords: Rice, EMS, Mutants, Barak Cenana, salt tolerance

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