Published date: Feb 11 2020

Journal Title: KnE Life Sciences

Issue title: The 2019 International Conference on Biotechnology and Life Sciences (IC-BIOLIS)

Pages:

DOI: 10.18502/kls.v5i2.6437

Ni Wayan Deswiniyanti deswiniyanti@undhirabali.ac.idFaculty of Health, Science and Technology, Dhyana Pura University, Badung, Bali 80361

Ni Kadek Dwipayani LestariFaculty of Health, Science and Technology, Dhyana Pura University, Badung, Bali 80361

Lily (Lilium longiflorum) is a perennial herbaceous plant with white trumpet-shaped flowers, fragrant and bulbous. In vitro culture through bulbs is one of way propagation of lily plants, but it requires a long time and only produces limited plants. In vitro propagation is a very promising technique for plant propagation because it can produce a lot of plant seeds in a short time. Bulbs are one of the fastest explants for growing shoots in lilies, but it is not known for certain which cuts of explants from bulb scales are best for multiplying in vitro. This study aims to determine the effect of lily bulb explants and the concentration of NAA and BAP growth regulators on the growth of lily bulb explants. The best results were obtained on the base and middle cuts explant of bulb scales compared to the tip cuts explant ones. The best results of the growing percentage, the number of shoots and the best growing time are shown in the combination treatment of growth regulator 1 mg L⁻¹ NAA and 1 mg L⁻¹ BAP. The optimum results on the number of micro bulbs were found in the treatment of growth regulators 0.5 mg L⁻¹ NAA and 1 mg L⁻¹ BAP. The best results of the average time formed micro bulb was in the treatment of 1 mg L⁻¹ NAA and BAP with middle explant cuts, and treatment concentrations of 0.5 mg L⁻¹ NAA and BAP in the base explant section. The base and middle bulb explants are able to regenerate or grow higher shoots. This is caused by the presence of endogenous natural auxin and the spread of auxin in plant parts not in the same amount. Therefore when added to the exogenous growth regulator such as auxin or cytokines to culture media will further trigger the formation of micro tubers more quickly,. It can increase the concentration of endogenous growth regulators in cells, help growing process and developing tissue.

Keywords: Bulb, lily, micro bulbs, in vitro, shoots

[1] Miller, W. B. Easter and Hybrid Lily Production Principles and Practice. Portland Oregon: Timber Press Inc. 1992.


[2] Hoesen DSH., Gandawidjaya J. Lili Bunga Pegunungan. Buletin Kebun Raya Bogor 6(6), 141-147. 1985. [3] Priyono, Winarsih. Pengaruh Arah Dan Ukuran Potongan Sisik Umbi Kerk Lily (Lilium longiflorum Thunb.) Terhadap Pembentukan Tunas Mikro Dan Bulblet Secara In Vitro. Berita Biologi. 5(1):85-92. 2000.


[4] Robb SM. The culture of excised tissue from bulb scales of Lilium speciosum Thun. Journal of Experimental Botany 8(24), 348-352. 1957.


[5] Takayama S., Misawa M. Regulation of Organ Formation by Cytokinin and Auxin in Lilium Bulbscale Grown in vitro. Plant Cell Physio. 23, 67-74. 1982.


[6] Liu L and Burger DW. In Vitro Propagation of Easter Lily from Pedicels. Hort.Sci. 21(6), 1437-1438. 1986.


[7] Winarsih S, Priyono, Zaenudin. Pengaruh Zat Pengatur
tumbuh terhadap Perbanyakan Kerk lili secara in vitro. Jurnal Hortikultura 8(3), 1145-1152. 1998.


[8] Luong MX., Ket NV. Rapid Multiplication of Lilium by Tissue Culture. Proceedings of the Southeast Asian Regional Workshop on Propagation Technique for Commercial Crop of the Tropic. Ho Chi Minh City, Vietnam, 7-12 February 1993. IFS&BRC. him. 180-189. 1993


[9] Suharjo UKJ, Herison C, Fahrurrozi. Keragaman tanaman kentang varitas Atlantik dan Granola di dataran medium (600 m dpl) Bengkulu pasca iradiasi sinar gammat. Akta Agrosia. vol 13(1): 82-88. 2010.


[10] Zakaria RA, Zanjani BM, Sedghi E. Effect of in vitro chitosan application on growth and minituber yield of Solanum tuberosum L. Plant Soil Env. vol 55 (6): 252-256. 2009.


[11] Fufa M and Diro M. Microtuber induction of two potato (Solanum tubersoum L.) varities. Adv. Crop Sci. Tech. vol 2(2): 122. 2014.


[12] Dhaka M., Nailwal TK. High efficienty macropropagation of potato (Solanum tuberosum L.) cv. Kufru Jyoti in Kumaun Hills. J. Plant Breeding and Crop Sci. vol 7(7): 203-210. 2015.


[13] Kanchanapooma K., Tidarat P., Wimon W., Kamnoon K. Regeneration of lily (Lilium longiflorum ‘Easter lily’) by callus derived from leaf explants cultured in vitro. ScienceAsia 37 (2011): 373–376. 2011.


[14] Mantell SH, Matthews JA, McKee RA. Principal of Plant Biotechnology. Blackwell Scientific Publication. Oxford. 269p. 1985.


[15] Reshi, N. A., Sudarshana M. S., Girish H. V. In Vitro Micropropagation of Anisochilus carnosus (L) Wall. Journal of Applied Pharmaceutical Science Vol. 7 (07), pp. 098-102. 2017.


[16] Gardner, F.P.,R.B. Pearce, dan R.L. Mitchell. Fisiologi Tanaman Budidaya. Jakarta: UI Press. 1991.


[17] Sun, L., Zhongze Z., Kemeng C. Micropropagation from in vitro cultured bulb scales of Lilium lancifolium. Life Science Journal 10 (2); 2689-2692. 2013.


[18] Yun SY., Lee MS., Lim SC., Shin JD. Micropropagation of Heloniopsis orientalis (Thunb.).C. Tanaka in-vitro. Kor J. Plant Tiss Cult. 27:197-202. 2000.


[19] Thomas, TD., Chaturvedi, R. Endosperm culture: a novel method for triploid plant production’, Plant Cell Tissue and Organ Culture, vol. 93, pp. 1-14. 2008.


[20] Fonnesbech, M. Growth hormone and propagation of Cymbidium in vitro. Physiol. Plant., vol. 27, pp. 310-16. 1992.


[21] Morel, GM. Clonal multiplication of orchid In Withner, CL(ed.)., New York: The orchids scientific studies, Wiley-Interscience Publication, John Wiley & Sons pp. 169-22. 1974.


[22] Lan, TH, Hong, PL, Huang, CC, Chang, WC & Lin, CS. High frequency direct somatic embryogenesis from leaf tissues of Drimiopsis kirkii Baker (giant squill). In vitro Cell. Dev. Biol. Plant., vol. 45, pp. 44-7. 2009.


[23] Lestari, E. G.Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman Melalui Kultur Jaringan. Jurnal AgroBiogen.,vol.7,no.1, pp.63-68, 2011.


[24] Askari, N., Richard G.F. Visser, Geert-Jan De K. Growth of Lily Bulblets In Vitro, a Review. International Journal of Horticultural Science and Technology Vol. 5(2); pp 133-143. 2018.


[25] Joseph, S., Lekha K., Narmatha BV. Micropropagation of Cyrtanthus mackenii Hook. f from tri scales. European Journal of Biotechnology and Bioscience. Volume 4(8): 67-72. 2016.