Published date: Nov 25 2019

Journal Title: KnE Life Sciences

Issue title: International Scientific and Practical Conference “AgroSMART – Smart Solutions for Agriculture”

Pages: 782–791

DOI: 10.18502/kls.v4i14.5675

Yu N Alekhinmaxim.zhukoff2015@yandex.ruFederal State Budgetary Scientific Institution All-Russian Veterinary Research Institute of Pathology, Pharmacology, and Therapy

A Yu LebedevaFederal State Budgetary Scientific Institution All-Russian Veterinary Research Institute of Pathology, Pharmacology, and Therapy

M S ZhukovFederal State Budgetary Scientific Institution All-Russian Veterinary Research Institute of Pathology, Pharmacology, and Therapy

V I MorgunovaFederal State Budgetary Scientific Institution All-Russian Veterinary Research Institute of Pathology, Pharmacology, and Therapy

L N KashirinaFederal State Budgetary Scientific Institution All-Russian Veterinary Research Institute of Pathology, Pharmacology, and Therapy

Yu E SukhanovaFederal State Budgetary Scientific Institution All-Russian Veterinary Research Institute of Pathology, Pharmacology, and Therapy

In the conditions of a dairy farm with the help of the standard clinical, instrumental and laboratory methods the research on particularities of Eritrocytic parameters of the blood of calves (n=299) with different birth weights was carried out. It is shown that Holstein calves with normal intrauterine growth, born with body weight from 36.5 to 29 kg, have no reliable differences in red blood cell count. With a 1 kg reduction in body weight, there is a trend towards hyperchromia, but an increase in weight deficiency promotes of hypovolemia, hypochromia, and polycythaemia. In newborns with a body weight of 27.9–26.9 kg, polycythaemia is derived from hypovolemia, but, with a more pronounced weight deficiency, the role of erythrogenesis disorder in the pathogenesis of the syndrome increases. Hypochromia is a consequence of hematopoietic organs dysfunction and transmembrane loss of hemoglobin. Herein membrane destruction is caused by the increase in the content of toxical metabolites in the blood (sorptivity of red blood cells by 10–12 percent), and by a higher level of adrenaline (modification coefficient of membranes by adrenaline by 20–30 %). This indicates that the response of the fetus to the factors that inhibit its development is similar to a metabolic response against stress. At strong underweight body (b.w. less than 25 kg) exhausting of adaptive capability is observed with the increase in the blood level of toxical metabolites (sorptivity of red blood cells by 15 %), level of membrane destruction (level of ectoglobular hemoglobin in 2,8 times), and heterogeneity of red blood cells. Thus, in newborns with a body weight of less than 29 kg, the significant disturbances in the structure and functions of red blood cells were revealed, which gave grounds for stating that they had antenatal hypotrophy. Herewith, the severity of hematological changes depends on the degree of weight deficiency. Initially, it is hypovolemia and the resulting polycythemia, but, erythropoiesis disorders, and destruction of their membranes with increased polycythemia, and the development of hypochromia occur with the increasing severity of hypotrophy.

[1] Kraskova, E.V. (2017). Main indicators of hematopoiesis in hypoplastic anemia in newborn calves. Vestnik Altaiskogo ASU, vol. 7(153), pp. 120–124.

[2] Mohri, M., Poorsina, S, Sedaghat, R. (2010). Effect of parenteral supply of iron on RBC parameters, performance, and health in neonatal dairy calve. Biological TraceElement Research, vol. 136, no. 1, pp. 33–39.

[3] Eremina, I.Y., Makarskaya, G.V., Tarskikh, S.V. (2010). Age peculiarities of oxygen metabolism of blood cells of cattle. Vestnik KrasASU, no. 11, pp. 128–135.

[4] Gavrilov, O.K., Kozinets, G.I., Chernyak, N.B. (2016). Bone marrow and peripheral blood cells. Moscow: Medicine.

[5] Moroz, V.V., Golubev, A.M., Afanas'ev, A.V. (2012). Structure and function of erythrocyte in normal and critical states. Obshch. Reanimatol., vol. 3, no. 1, pp. 52–60.

[6] Borovskaya, M.K., Kuznetsova, E.E., Gorokhova, V.G. et al. (2010). Structural and functional characteristics of the erythrocyte membrane and its change at pathologies of the different genesis of Acta Biomedica Scientifica, no. 3(73), pp. 334–354.

[7] Omar, S., Hadj Taeib, S., Kanoun, F. et al. (2010). Erythrocyte abnormalities in thyroid dysfunction. Tunis Med., vol. 88(11), pp. 783–788.

[8] Alekhin, Yu.N. (2000). Endogenous intoxications in animals and their diagnostics: (Methodical recommendations). Voronezh.

[9] Tonkoshkurova, O.A., Dmitriev, A.I., Dmitrieva, R.E. (1996). Determination of concentration of extra-erythrocytic hemoglobin of plasma (serum) by hemoglobin cyanide method. Clinical Laboratory Diagnostics, no. 2, pp. 21–22.

[10] Alekhine, Yu.N. (2013). Methods of diagnosis of perinatal pathology in cattle: a methodological guide. Voronezh.

[11] Mandel, D. (2011). Neonatal polycythemia: critical review and a consensus statement of the Israeli Neonatology Association. Acta Pædiatrica, vol. 100, pp. 1290–1296.

[12] Devanathan, S., Yao, Z., Salamon, Z. et al. (2004). Plasmon-Waveguide Resonance Studies of Ligand Binding to the Human β-- Adrenergic Receptor. Biochemistry, vol. 43, no. 11, pp. 3280–3288.

[13] Molenaar, P. (2003). The ``state'' of beta-adrenoceptors. Brit. Journal Pharmacol., vol. 140, no. 1, pp. 1–2.

[14] Krysova, A.V., Tsirkin, V.I., Kunshin, A.A. (2011). The role of α and β-adrenal receptors in the realization of adrenaline's ability to change the osmotic resistance of erythrocytes of pregnant women. Vyatka Medical Journal, no. 3-4, pp. 8–13.

[15] Sirota, T.V. (2013). Use nitroblue tetrazolium in the reaction of autooxidation of adrenaline for determination of activity superoxide dismutases. Biomedical chemistry, vol. 59, no. 4, pp. 399–410.

[16] Lewis, S.M., Bane, B., Bates, E. (2009). Practical and laboratory hematology. Moscow: GEOTAR-Media.

[17] Pavlov, A.D. (2016). Regulation of erythropoiesis. Moscow: Medicine.

[18] Fernandez, E.F., Watterberg, K.L. (2009). Relative adrenal insufficiency in the preterm and term infant. Journal of Perinatology, vol. 29, pp. 44–49.

[19] Shaybak, L.N., Katkova, E.V., Doroshenko, E.M. (2010). Umbilical cord blood serum catecholamines for premature and premature infants. Journal of GrSMU, no. 2, pp. 152–154.

[20] Ushakov, I.B., Stemberg, A.S., Shafirkin, A.V. (2007). Reactivity and resistance of mammalian organisms. Moscow, Moscow: Science.

[21] Rebrova, O.Yu. (2002). Statistical analysis of medical data. Moscow: MediaSphere.