Published date: Mar 25 2019

Journal Title: KnE Life Sciences

Issue title: The 1st International Conference on Health, Technology and Life Sciences (ICO-HELICS)

Pages: 103–108

DOI: 10.18502/kls.v4i12.4162

Sari Y .sariyulia15@yahoo.co.id

Suryawati B .

Yudhani R D .

Artama W T .

Giardia sp. is a gastrointestinal protozoan that is common in mammals and causes giardiasis. Detection of parasitic infections in stool samples can use different methods such as identification of Giardia and trophozoite cysts using a light microscope (saline and iodine) and gene amplification gdh. The aim of this study was to compare the detection of direct microscopic Giardia and PCR in healthy people at risk for Giardiasis
in the Bedog watershed, Sleman, DIY. The results of the examination using positive Giardia samples from microscopy were obtained at 4% (4/10). While the PCR results are 7% (7/100). The microscopic method and PCR did not have a significant difference in PCR so that certain microscopic conditions can still be recommended as a basic method in detecting Giardia cysts and trophozoites. The sensitivity and specificity of the direct microscope were 96.9%, and 100%, respectively. The sensitivity and specificity of molecular analysis (PCR) were 97.14% and 100%, respectively. Although PCR detection is more specific than microscopic, in this case, the microscope method can still be used as an initial detection method. While the important advantages of PCR testing, its ability to directly distinguish between different Giardia genotypes, will help deal with cases of Giardiasis. The results of this study indicate that confirmation using the PCR technique can strengthen microscope detection.

[1] Dunn N and Juergens, A L 2018 Giardiasis in StatPearls Treasure Island.


[2] Soares R and Tasca T 2016 J. Microbiol. Methods 129 98-102


[3] Einarsson E, Ma’ayeh S and Svard S G 2016 Curr. Opin. Microbiol 34 47-52


[4] Cama V A and Mathison B A 2015 Clin. Lab. Med 35 423-44


[5] Huang D B and White A C 2006 Gastroenterol Clin. North. Am 35 291-314


[6] Rodriguez-Morales A J, Granados-Alvarez S, Escudero-Quintero H, Vera-Polania F., Mondragon-Cardona A, Diaz-Quijano F A et al 2016 Int. J. Infect. Dis 49 204-9.


[7] Manser M, Granlund M, Edwards H, Saez A, Petersen E, Evengard B et al 2014 Clin. Microbiol. Infect 20 65-71


[8] Adam EA, Yoder JS, Gould LH, Hlavsa MC, and Gargano J W 2016 Epidemio.l Infect 144 2790-801


[9] El-Nahas H A, Salem D A, El-Henawy A A, El-Nimr H I, Abdel-Ghaffar H A and ElMeadawy A M 2013 Cytometry. B. Clin. Cytom 84 44-9


[10] Hawash Y, Ghonaim M M, and Al-Shehri S S 2016 Korean J. Parasitol. 54 1-8


[11] Hijjawi N, Yang R, Hatmal M, Yassin Y, Mharib T, Mukbel R, et al 2018 Exp. Parasitol 185 23-28


[12] Adeyemo F E,Singh G,Reddy P, and Stenstrom T A 2018 Acta Trop 184 15-28


[13] Johnston S P, Ballard M M, Beach M J, Causer L and Wilkins P P 2003 J Clin Microbiol 41 623-6


[14] Gotfred-Rasmussen H, Lund M, Enemark H L, Erlandsen M and Petersen E 2016 Diagn. Microbiol. Infect. Dis 84 187-90


[15] Asher A J, Waldron L S and Power M L 2012 Parasitol. Res 110 853-8


[16] Ignatius R, Gahutu J B, Klotz C, Steininger C, Shyirambere C, Lyng M et al 2012 PLoS. Negl. Trop. Dis 6 1677


[17] Koehler A V, Jex A R, Haydon S R, Stevens M A and Gasser R B 2014 Biotechnol Adv 32 280-9


[18] Johnston A R, Gillespie T R, Rwego I B, Mclachlan T L, Kent A D and Goldberg T L 2010 PLoS Negl. Trop. Dis 4 683


[19] Helmy Y A, Klotz C, Wilking H, Krucken J, Nockler K, Von Samson-Himmelstjerna G et al 2014 Parasit Vectors 7 321


[20] Shin J H, Lee S E, Kim T S, Ma D W, Chai J Y and Shin E H 2016 Korean J Parasitolb54 631-36.