Published date: Sep 20 2015

Journal Title: KnE Life Sciences

Issue title: International Conference on Biological Sciences (ICBS-2013)

Pages: 476-479

DOI: 10.18502/kls.v2i1.194

Ganies Riza Aristya - ganies_riza@ugm.ac.id

Rezika Alyza

Rosyidatul Khoiroh

Budi Setiadi Daryono

The cultivated strawberries, Fragaria x ananassa and Fragaria vesca, are the most economically-important softfruit species. F x ananassa and F vesca, both diploid (2n=2x=14) relatives of the commercial octoploid strawberry, are an attractive model for functional genomics research in Rosaceae. Its small genome size, short reproductive cycle, and facile vegetative and seed propagation make F. x annassa and F.vesca a promising candidates for forward and reverse genetics experiments. In order to determine their genetic differences in more detail, chromosome characterization of the two strawberry cultivars was investigated. A method used for chromosome slides in this research was a squash method with modification in pre-treatment. The result showed Fragaria x ananassa had (2n = 4x = 28) chromosome number is 28 and Fragaria vesca had (2n = 2x = 14) chromosome number is 14. The time of mitotic that both strawberry cultivars was similar at 7 to 8.30 am. In addition, mixoploid cells were found in both strawberry cultivar indicating that these cultivars had been treated by mutagenic agents for a breeding program.

Keywords : Fragaria, chromosome, mitotic

Ahmadi, H., and R.S. Bringhurst. 1992. Breeding strawberries at the decaploid level. J Am Soc Hort Sci 117:856–862
Bauer, A. 1993. Progress in breeding decaploid Fragaria x vescana. 2nd Intl Strawberry Symp, Beltsville, Maryland, USA, 13–18 September 1992. Acta Hort 348:60–64
Bors, R.H. 2000. A streamlined synthetic octoploid system that emphasizes Fragaria vesca as a bridge species. PhD Thesis, Univof Guelph, Ontario, Canada
Byrne, D., and G. Jelenkovi´c. 1976. Cytological diploidization in the cultivated strawberry Fragaria ×ananassa. Canadian Journal of Genetics Cytology 18:653–659
Darrow, G.M. 1966. The strawberry: History, breeding and physiology. Holt, Rinehart and Winston, USA
Evans, W.D. 1977. The use of synthetic octoploids in strawberry breeding. Euphytica 26:497– 503
Fedorova, N.J. 1946. Crossability and phylogenetic relations in the main European species of Fragaria. Compilation of the National Academy of Sciences USSR 52: 545–547
Ichijima, K. 1926. Cytological and genetic studies on Fragaria. Genetics 11: 590–603
Iwatsubo, Y., and N. Naruhash. 1989. Karyotypes of three species of Fragaria (Rosaceae). Cytologia 54: 493–497
Lim, K.Y. 2004. Karyotype and ribosomal gene mapping in Fragaria vesca L. Acta Horticulturae 649: 103–106
Oosumi, T., H.A. Gruszewski, L.A. Blischak, A.J. Baxter, P.A. Wadl, J.L. Shuman, R.E. Veilleux, and V. Shulaev . 2006. High-efficiency transformation of the diploid strawberry (Fragaria vesca) for functional genomics. Planta 223: 1219–1230
Scott, D.H. 1951. Cytological studies on polyploids derived from tetraploid Fragaria vesca and cultivated strawberries. Genetics 36:311–325
Senanayake, Y.D.A., and R.S. Bringhurst. 1967. Origin of Fragaria polyploids I. Cytological analysis. American Journal of Botany 54: 221–228
Yarnell, S.H. 1928. Notes on the somatic chromosomes of the seven-chromosome group of Fragaria. Genetics 14: 78–83.