KnE Life Sciences

ISSN: 2413-0877

The latest conference proceedings on life sciences, medicine and pharmacology.

The Effect of Cinnamomum Burmanii Ethanol Extract on Isoniazid-induced Serum Levels of Serum Glutamate Piruvate Transaminase (SGPT) Wistar Strain Male Rats

Published date: Oct 04 2024

Journal Title: KnE Life Sciences

Issue title: 4th International Conference in Social Science (4th ICONISS): Healthcare

Pages: 41–51

DOI: 10.18502/kls.v8i2.17357

Authors:

Evi SoviaEvi.sovia@lecture.unjani.ac.idDepartment of Pharmacology, Faculty of Medicine, Jenderal Achmad Yani University, Cimahi

Gusti Ayu SintaDepartment of Physiology, Faculty of Medicine, Jenderal Achmad Yani University, Cimahi

Dinda Annisa AMedicine Study Program, Faculty of Medicine, Jendral Achmad Yani University, Cimahi

Indarti TrimurtiniDepartment of Anatomy, Faculty of Medicine, Jenderal Achmad Yani University, Cimahi

Abstract:

Hepatotoxicity can be caused by excessive drug use, triggered by increased oxidative stress which is considered as the initial mechanism of Drug Induced Liver Injury (DILI). One of the causes of DILI is Isoniazid. One of the plants acting as a hepatoprotector in protecting liver cells is cinnamon (Cinamommum burmanii). This study aims to determine the effect of 96% ethanolic cinnamon extract as a hepatoprotector against an increase in Alanine Transaminase (ALT) levels induced by isoniazid. The parameters used to assess liver damage were rat plasma ALT. The sample used in this study was rat plasma, which was taken through the retro orbital sinus. This research is an experimental study with a sample of 25 experimental animals divided into five groups, namely negative control (K1), positive control l (K2), and experimental group with cinnamon ethanolic extract at a dose of 100 mg/kgbw (K3), 200 mg/kgbw(K4), and 400 mg/kgbw(K5). The research used a post-test-only control group design. The results were analyzed using the One-way ANOVA test followed by the post hoc Tukey test. The results proved that cinnamon ethanolic extract at doses of 100 mg/kgbw (P = 0.029), 200 mg/kgbw (P = 0.001), and 400 mg/kgbw (P = 0.000) was effective in reducing plasma SGPT levels in isoniazid-induced rats when compared with the positive control group (K2). The most effective dose was at 200 mg/kgbw (K4). Thus, this proves that all of the doses in experimental groups have a hepatoprotective effect against isoniazid-induced liver damage.

Keywords: Alanine Transaminase (ALT), cinnamon ethanol extract, Drug Induced Liver Injury (DILI), isoniazid

References:

[1] Lee LN, Huang CT, Hsu CL, Chang HC, Jan IS, Liu JL, et al. Mitochondrial DNA Variants in Patients with Liver Injury Due to Anti-Tuberculosis Drugs. J Clin Med. 2019 Aug;8(8):1207.

[2] Yew WW, Chang KC, Chan DP. Oxidative Stress and First-Line Antituberculosis Drug- Induced Hepatotoxicity. Antimicrob Agents Chemother. 2018 Jul;62(8):e02637–17.

[3] Villanueva-Paz M, Morán L, López-Alcántara N, Freixo C, Andrade RJ, Lucena MI, et al. Oxidative Stress in Drug-Induced Liver Injury (DILI): From Mechanisms to Biomarkers for Use in Clinical Practice. Antioxidants. 2021 Mar;10(3):390.

[4] Robiyanto R, Liana J, Purwanti NU. Kejadian Obat-Obatan Penginduksi Kerusakan Liver pada Pasien Sirosis Rawat Inap di RSUD Dokter Soedarso Kalimantan Barat. Jurnal Sains Farmasi & Klinis. 2019;6(3):274–85.

[5] Andri J, Febriawati H, Randi Y, J H, Setyawati AD. J H, Setyawati AD. Penatalaksanaan Pengobatan Tuberkulosis Paru. Jurnal Kesmas Asclepius. 2020;2(2):73–80.

[6] Somayana G, Studi P, Dokter P, Kedokteran F, Udayana U, Penyakit B, et al. Prevalensi dan Gambaran Umum Drug-Induced Liver Injury Akibat Obat Anti Tuberkulosis pada Pasien Tuberkulosis RSUP Sanglah Denpasar Periode Agustus 2016 – Juli 2017. Jurnal Medika Udayana. 2019;8(9).

[7] Samuel Pola Karta Sembiring. Indonesia bebas tuberkulosis. Sukabumi: CV Jejak; 2019.

[8] Katzung BG, Masters SB, Trevor AJ. Basic & Clinical Pharmacology. 12th ed. New York: McGraw Hill Education; 2012.

[9] Nisrina H, Hilmi IL. Description of Side Effects of Using Antituberculosis Drug in Pulmonary Tuberculosis Patients : Literature Review. Jurnal eduhealth. 2022;13(02):684–8.

[10] Lei S, Gu R, Ma X. Clinical perspectives of isoniazid-induced liver injury * [Internet]. Liver Res. 2021;5(2):45–52.

[11] Helmalia AW, Putrid P, Dirpan A. Putrid, Dirpan A. Potensi Rempah-Rempah Tradisional Sebagai Sumber Antioksidan Alami Untuk Bahan Baku Pangan Fungsional). Canrea Journal. 2019;2(1):26–31.

[12] Idris H, Mayura E. Teknologi Budidaya dan Pasca Panen Kayu Manis (Cinnamomum burmanii). In: Sirkuler Informasi Teknologi Tanman Rempah dan Obat. Bogor: Balai Penelitian Tanaman Rempah dan Obat; 2019.

[13] Rahayu L, Yantih N, Supomo Y. Analisis SGPT dan SGOT pada Tikus yang Diinduksi Isoniazid untuk Penentuan Dosis dan Karakteristik Hepatoprotektif Air Buah Nanas (Ananas comosus L. Merr) Mentah. Jurnal Ilmu Kefarmasian Indonesia. 2018;16(1):100–6.

[14] Perwitasari DA, Darmawan E, Mulyani UA, Vlies PV, Alffenaar JC, Atthobar J, et al. Polymorphisms of NAT2, CYP2E1, GST, and HLA related to drug-induced liver injury in indonesian tuberculosis patients. Int J Mycobacteriol. 2018;7(4):380–6.

[15] Noor SM, Dharmayanti I, Wahyuwardani S, Muharsini S, Cahyaningsih T, Widianingrum Y, et al. Penanganan Rodensia dalam Penelitian Susuai Kaidah Kesejahteraan Hewan. Jakarta: IAARD Press (Badan Penelitian dan Pengembangan Pertanian); 2022.

[16] Prasetyorini, Utami NF, Yulianita, Novitasari N, Fitriyani W. Potensi Ekstrak Refluks Kulit Batang Kayu Manis (Cinnamomum burmannii) Sebagai Antijamur Candida albicans dan Candida tropicalis. FITOFARMAKA : Jurnal Ilmiah Farmasi. 2021;11(2):164–78.

[17] Leary S, Underwood W, Lilly E, Anthony R, Cartner S, Corey D, et al. AVMA Guidelines for the Euthanasia of Animals. 2013 Edition. Schaumburg: American Veterinary Medical Association; 2013.

[18] Sediarso SE, Efendi K. Ekstrak Biji Petai (Parkia Spesiosa Hassk) Sebagai Hepatoprotektor Berdasarkan Kadar SGPT, SGOT Dan Histologi Hati Tikus Putih Jantan Yang Diinduksi CCL4. Jurnal Ilmiah Kesehatan. 2018;10(2):181–9.

[19] Nuari DA, Qowwiyah A, Eksyawati D. Aktivitas Hepatoprotektif Ektrak Etanol Rebung Bambu Kuning (Bambusa Vulgaris Schard) pada Tikus Putih Jantan Galur Wistar. Jurnal Ilmiah Farmako Bahari. 2018;9(2):16–22.

[20] Sunyoto M, Arifin HR, Kurniati D. Rempah yang Mendunia. Bandung: Bitread Publishing; 2019.

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