Published date: Sep 20 2015

Journal Title: KnE Life Sciences

Issue title: International Conference on Biological Sciences (ICBS-2013)

Pages: 274-277

DOI: 10.18502/kls.v2i1.157

Rita Rizky Rahmawatitatarizky91@gmail.comDepartment of Biology, Faculty of Mathematic and Natural Science State University of Malang, Jl. Semarang 5, Malang

Emy KusumawatiDepartment of Biology, Faculty of Mathematic and Natural Science State University of Malang, Jl. Semarang 5, Malang

Lulus Putri AnindaDepartment of Biology, Faculty of Mathematic and Natural Science State University of Malang, Jl. Semarang 5, Malang

Miftahul MushlihFaculty of Biology, Gadjah Mada University, Yogyakarta

Abdul GofurDepartment of Biology, Faculty of Mathematic and Natural Science State University of Malang, Jl. Semarang 5, Malang

Dwi ListyoriniDepartment of Biology, Faculty of Mathematic and Natural Science State University of Malang, Jl. Semarang 5, Malang

Javan Langur (Trachypithecus auratus) is an endemic langur in Indonesia which classified as vulnerable primate. Some researches divided Javan Langur species into some subspecies, yet was not described properly. Cytochrome-b sequence from mitochondrial DNA is able to determine samples between and intra-species as well. This study aimed to identify the Javan Langur (Trachypithecus auratus) samples named Andin (@&) and Bobby (B&), both are rehabilitated in Javan Langur Center (JLC). The amplification of Cytochrome-b gene from whole blood DNA with PCR technique using forward primer L151625’­CTTCCATGAGGACAAATATC-3’ and reverse primer Rmuc15’-GTGGAGTATAGGTATGATTGC-3’. The phylogenetic tree reconstruction based on MEGA 5 using maximum likelihood (ML) method and pairwise distance analysis with Kimura-2 parameter model resulted that Andin and Bobby are in the same group with
T. a. auratus. Andin is in the same clade with T. a. auratus haplotype aaF, while Bobby in the same clade T.
a. auratus and T. a. auratus haplotype aaC. We concluded that both of them belong to T. a. auratus subspecies.

Keywords: Javan Langur (Trachypithecus auratus), Javan Langur Center (JLC), Cytochrome-b

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