Published date: Mar 27 2024

Journal Title: KnE Life Sciences

Issue title: International Conference On Mathematics And Science Education (ICMScE 2022): Life Sciences

Pages: 97–106

DOI: 10.18502/kls.v8i1.15403

Dalia Sukmawatidalia-sukmawati@unj.ac.idBiology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia

Alza Kirana ThaharahBiology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia

Esti KomariahBiology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia

Herawati HerawatiBiology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia

Vicky TheodoraBiology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia

Atin SupiyaniBiology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia

Shabrina Nida Al HusnaDepartment of Microbiology, School of Life Sciences and Technology, Institut Teknologi Bandung, Indonesia

Nuniek Ina RatnaningtyasDepartment of Biology,Faculty of Biology, Jenderal Soedirman University, Dr. Suparno Road, Number 63, Karangwangkal, North Purwokerto, Banyumas Regency, Central Java, 53122, Indonesia

Hesham El EnshasyInstitute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia

Daniel Joe DailinInstitute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia

Dark Septate Endophytes (DSE) are a group of ascomycetes that live in intracellular and extracellular root tissue to facilitate plant growth and stress tolerance in extreme environments. However, little is known about the DSE fungi isolated from certain plant roots such as Pteris vittata, especially under drought condition. Pteris vittata is known for its ability to live in various types of substrates and ecosystems. In this study, we obtained DSE fungi from the fern roots Pteris vittata collected from the area of Universitas Negeri Jakarta. DSE isolation was carried out by inoculating the Pteris vittata fern roots with a size of 0.5 cm on the surface of sterilized PDA media for 5-7 days at 27∘C. Observations were made every 24 hours using a stereo microscope to see the first hyphae appeared from the plant roots. The results exhibited 13 samples of roots with hyphae colonization and were suspected to be DSE fungi. Of the 13 root isolates, only 3 isolate (PP2, PP4A, and PPB) showed the DSE growth (23%) with melanin pigment. The morphological characteristics of endophytic DSE fungi collected from Pteris vittata roots represented septate hyphae, brownish to black colony color, a growing zone, and a velvety texture. For the isolate PP2, it showed sclerotia while for the isolate PP4, it exhibited light brown colonies.

Keywords: isolation, DSE, ferns, pteris vittata, roots

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