KnE Life Sciences

ISSN: 2413-0877

The latest conference proceedings on life sciences, medicine and pharmacology.

Antagonistic Activity of Cellulase-Producing Yeasts Isolated from Cocoa Fermentation Against Pathogenic Molds Collected from Damaged Cocoa Fruits

Published date: Mar 27 2024

Journal Title: KnE Life Sciences

Issue title: International Conference On Mathematics And Science Education (ICMScE 2022): Life Sciences

Pages: 85–96

DOI: 10.18502/kls.v8i1.15401

Authors:

Dalia Sukmawati - dalia-Sukmawati@unj.ac.id - https://orcid.org/0000-0001-9641-9321

Alika Firhandini

Umi Khumaiya

Dwi Ayu Komsiatun

Syifa Aulia Gunadi

Atin Supiyani

Dwi Ningsih Susilowati

Shabrina Nida Al Husna

Hesham El Enshasy

Daniel Joe Dailin

Catur Sriherwanto

Abstract:

The presence of pathogenic molds in post-harvested cocoa beans poses a significant problem to most Indonesian farmers. Pathogenic molds secrete phytopathogenic toxins which cause rotting, seriously damaging the cocoa fruits. The common pathogenic molds found in damaged cocoa beans are Phytophthora palmivora and Phytophthora megakarya. These molds are so far controlled by using chemical pesticides, whose long-term use is potentially harmful to human health and the environment. Thus, a healthier and safer alternative is needed, especially using biological agents such as yeasts. Some yeasts produce cellulose-degrading enzymes that can possibly break down the fungal cell wall which is composed mostly of the polysaccharide’s chitin and glucan. In this study, yeasts were isolated during the cocoa fermentation process, followed by screening for cellulolytic activity using direct planting and paste method, and subsequent antagonistic testing by double culture technique. Out of 128 yeast isolates, 77 possessed cellulase activity, with 6 of them having the highest activity index. Antagonistic activity test of these 6 isolates (C1.0.4, C1.1.3, C2.3.10, C2.3.14, C3.5.11, and C3.3.1) against the pathogenic molds resulted in isolate C3.3.1 showing the highest inhibition percentage from the 2

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